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Literature DB >> 4887512

Phosphatidic acid synthesis in Escherichia coli.

Abstract

The kinetic properties of acyl-coenzyme A (CoA): l-alpha-glycerol-phosphate trans-acylase (EC 2.3.1.15) from Escherichia coli were studied. At 10 C, a temperature at which the reaction was proportional to time and enzyme concentration, the enzyme had an apparent K(m) of 60 mum for l-alpha-glycerol-phosphate. The curve describing the velocity of the reaction as a function of palmitoyl-CoA concentration was sigmoid but the plot of v(-1) versus [S](-3) gave a straight line. A K(m) of about 11 mum was calculated for palmitoyl-CoA. Adenosine triphosphate specifically inhibited the reaction, being a noncompetitive inhibitor in respect to l-alpha-glycerol phosphate. Inhibition only occurred with high concentrations of palmitoyl-CoA, and maximal inhibition was 60%.

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Year: 1969 PMID： 4887512 PMCID： PMC249850 DOI： 10.1128/jb.97.3.1321-1327.1969

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

19 in total

6. Biosynthesis of phosphatidic acid, lysophosphatidic acid, diglyceride, and triglyceride by fatty acyltransferase pathways in Escherichia coli.

Authors: R A Pieringer; H Bonner; R S Kunnes
Journal: J Biol Chem Date: 1967-06-10 Impact factor: 5.157

7. Pathways for the synthesis of glycerophosphatides in Escherichia coli.

Authors: Y Y Chang; E P Kennedy
Journal: J Biol Chem Date: 1967-02-10 Impact factor: 5.157

8. Use of a filter-paper disk assay in the measurement of lipid biosynthesis.

Authors: H Goldfine
Journal: J Lipid Res Date: 1966-01 Impact factor: 5.922

9. [Potassium transport and phospholipid synthesis in Escherichia coli. I. The phospholipids of strains B 163 and B 525].

Authors: B Lubochinsky; J Meury; J Stolkowski
Journal: Bull Soc Chim Biol (Paris) Date: 1965

10. Phospholipid synthesis in Escherichia coli infected with T4 bacteriophages.

Authors: M H Furrow; L I Pizer
Journal: J Virol Date: 1968-06 Impact factor: 5.103

15 in total

Review 1. Physical properties of membrane lipids: biological relevance and regulation.

Authors: J E Cronan; E P Gelmann
Journal: Bacteriol Rev Date: 1975-09

2. Role of adenylate kinase in the regulation of macromolecular biosynthesis in a putative mutant of Escherichia coli defective in membrane phospholipid biosynthesis.

Authors: M Glaser; W Nulty; P R Vagelos
Journal: J Bacteriol Date: 1975-07 Impact factor: 3.490

6. Mutants of Escherichia coli with temperature-sensitive lesions in membrane phospholipid synthesis: genetic analysis of glycerol-3-phosphate acyltransferase mutants.

Authors: J E Cronan; G N Godson
Journal: Mol Gen Genet Date: 1972

Phosphatidic acid synthesis in Escherichia coli.

1. Protein measurement with the Folin phenol reagent.

2. The chromatographic identification of some biologically important phosphate esters.

3. The biosynthetic L-alpha-glycerol phosphate dehydrogenase of E. coli.

4. The effect of palmityl coenzyme A on glucose 6-phosphate dehydrogenase and other enzymes.

5. Palmityl-acyl carrier protein as acyl donor for complex lipid biosynthesis in Escherichia coli.

6. Biosynthesis of phosphatidic acid, lysophosphatidic acid, diglyceride, and triglyceride by fatty acyltransferase pathways in Escherichia coli.

7. Pathways for the synthesis of glycerophosphatides in Escherichia coli.

8. Use of a filter-paper disk assay in the measurement of lipid biosynthesis.

9. [Potassium transport and phospholipid synthesis in Escherichia coli. I. The phospholipids of strains B 163 and B 525].

10. Phospholipid synthesis in Escherichia coli infected with T4 bacteriophages.

Review 1. Physical properties of membrane lipids: biological relevance and regulation.

2. Role of adenylate kinase in the regulation of macromolecular biosynthesis in a putative mutant of Escherichia coli defective in membrane phospholipid biosynthesis.

3. Enzymology, genetics, and regulation of membrane phospholipid synthesis in Escherichia coli.

Review 4. Fatty acids of the genus Bacillus: an example of branched-chain preference.

5. Altered phospholipid metabolism in a sodium-sensitive mutant of Escherichia coli.

6. Mutants of Escherichia coli with temperature-sensitive lesions in membrane phospholipid synthesis: genetic analysis of glycerol-3-phosphate acyltransferase mutants.

7. Partial purification of glycerophosphate acyltransferase from Escherichia coli.

8. Regulation of phospholipid synthesis in Escherichia coli by guanosine tetraphosphate.

9. Composition of cardiolipin molecular species in Escherichia coli.

10. Acyl-acyl carrier protein as substrate of the acyltransferase of rat liver microsomes.