Literature DB >> 5900212

Use of a filter-paper disk assay in the measurement of lipid biosynthesis.

H Goldfine.   

Abstract

The precipitation by trichloroacetic acid (TCA) of radioactive lipids on disks of filter-paper, followed by extraction with TCA and water to remove soluble radioactive precursors, provides the means for a convenient assay for lipid biosynthesis. Large numbers of disks can be washed in the same vessel, dried, and assayed for radioactivity with liquid scintillation counting equipment. Extraction of disks with lipid solvents provides a convenient control for the possible presence of TCA- and water-insoluble, nonlipid products. The application of the method to the assay of cyclopropane fatty acid synthetase is shown.

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Year:  1966        PMID： 5900212

Source DB:  PubMed          Journal:  J Lipid Res        ISSN： 0022-2275            Impact factor:   5.922


  17 in total

1.  Cortisol induction of pulmonary maturation in the rabbit foetus. Its effects on enzymes related to phospholipid biosynthesis and on marker enzymes for subcellular organelles.

Authors:  F Possmayer; G Duwe; R Metcalfe; P J Stewart-DeHaan; C Wong; J L Heras; P G Harding
Journal:  Biochem J       Date:  1977-09-15       Impact factor: 3.857

2.  Stability of lactic Acid bacteria to freezing as related to their Fatty Acid composition.

Authors:  I Goldberg; L Eschar
Journal:  Appl Environ Microbiol       Date:  1977-03       Impact factor: 4.792

3.  Regulation of phosphatidylcholine and phosphatidylethanolamine synthesis in rat hepatocytes by 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR).

Authors:  Martin Houweling; Wil Klein; Math J H Geelen
Journal:  Biochem J       Date:  2002-02-15       Impact factor: 3.857

4.  Biosynthesis of glycolipids: incorporation of N-acetyl galactosamine by a rat brain particulate preparation.

Authors:  S Handa; R M Burton
Journal:  Lipids       Date:  1969-11       Impact factor: 1.880

5.  Selection and properties of Escherichia coli mutants defective in the synthesis of cyclopropane fatty acids.

Authors:  F Taylor; J E Cronan
Journal:  J Bacteriol       Date:  1976-02       Impact factor: 3.490

6.  Regulation of phospholipid synthesis in Escherichia coli by guanosine tetraphosphate.

Authors:  J P Merlie; L I Pizer
Journal:  J Bacteriol       Date:  1973-10       Impact factor: 3.490

7.  Multiple genes for membrane-bound phosphatases in Escherichia coli and their action on phospholipid precursors.

Authors:  T Icho; C R Raetz
Journal:  J Bacteriol       Date:  1983-02       Impact factor: 3.490

8.  Phosphatidylcholine metabolism in rat liver after partial hepatectomy. Evidence for increased activity and amount of CTP:phosphocholine cytidylyltransferase.

Authors:  M Houweling; L B Tijburg; H Jamil; D E Vance; C B Nyathi; W J Vaartjes; L M van Golde
Journal:  Biochem J       Date:  1991-09-01       Impact factor: 3.857

9.  Zinc deficiency-induced changes in the composition of microsomal membranes and in the enzymatic regulation of glycerolipid synthesis.

Authors:  S Clejan; V T Maddaiah; M Castro-Magana; P J Collipp
Journal:  Lipids       Date:  1981-06       Impact factor: 1.880

10.  Phosphatidic acid synthesis in Escherichia coli.

Authors:  M Kito; L I Pizer
Journal:  J Bacteriol       Date:  1969-03       Impact factor: 3.490
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