Literature DB >> 4772272

Molecular weight and amino acid composition of the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61.

J M Frère, J M Ghuysen, H R Perkins, M Nieto.   

Abstract

A procedure allowing the purification of milligram amounts of the exocellular dd-carboxypeptidase-transpeptidase from Streptomyces R61 to protein homogeneity (95% purity) is described. The isolated protein has a molecular weight of about 38000 and consists of one polypeptide chain. Its amino acid composition is presented.

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Year:  1973        PMID： 4772272      PMCID： PMC1165848          DOI： 10.1042/bj1350463

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  The oxidation of ribonuclease with performic acid.

Authors:  C H HIRS
Journal:  J Biol Chem       Date:  1956-04       Impact factor: 5.157

2.  Peptide inhibitors of Streptomyces DD-carboxypeptidases.

Authors:  M Nieto; H R Perkins; M Leyh-Bouille; J M Frère; J M Ghuysen
Journal:  Biochem J       Date:  1973-01       Impact factor: 3.857

3.  DD-carboxypeptidase-transpeptidase and killing site of beta-lactam antibiotics in Streptomyces strains R39, R61, and K11.

Authors:  J Dusart; A Marquet; J M Ghuysen; J M Frère; R Moreno; M Leyh-Bouille; K Johnson; C Lucchi; H R Perkins; M Nieto
Journal:  Antimicrob Agents Chemother       Date:  1973-02       Impact factor: 5.191

4.  Penicillin-sensitive DD-carboxypeptidases from Streptomyces strains R39 and K11.

Authors:  M Leyh-Bouille; M Nakel; J M Frère; K Johnson; J M Ghuysen; M Nieto; H R Perkins
Journal:  Biochemistry       Date:  1972-03-28       Impact factor: 3.162

5.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

6.  Penicillin-sensitive DD-carboxypeptidase from Streptomyces strain R 61.

Authors:  M Leyh-Bouille; J Coyette; J M Ghuysen; J Idczak; H R Perkins; M Nieto
Journal:  Biochemistry       Date:  1971-05-25       Impact factor: 3.162

7.  Transpeptidase activity of Streptomyces D-alanyl-D carboxypeptidases.

Authors:  J J Pollock; J M Ghuysen; R Linder; M R Salton; H R Perkins; M Nieto; M Leyh-Bouille; J M Frere; K Johnson
Journal:  Proc Natl Acad Sci U S A       Date:  1972-03       Impact factor: 11.205

8.  Streptomyces DD-carboxypeptidases as transpeptidases. The specificity for amino compounds acting as carboxyl acceptors.

Authors:  H R Perkins; M Nieto; J M Frére; M Leyh-Bouille; J M Ghuysen
Journal:  Biochem J       Date:  1973-04       Impact factor: 3.857

9.  Kinetics of concomitant transfer and hydrolysis reactions catalysed by the exocellular DD-carboxypeptidase-transpeptidase of streptomyces R61.

Authors:  J M Frère; J M Ghuysen; H R Perkins; M Nieto
Journal:  Biochem J       Date:  1973-11       Impact factor: 3.857

10.  Fluorescence and circular dichroism studies on the Streptomyces R61 DD-carboxypeptidase-transpeptidase. Penicillin binding by the enzyme.

Authors:  M Nieto; H R Perkins; J M Frère; J M Ghuysen
Journal:  Biochem J       Date:  1973-11       Impact factor: 3.857
  10 in total
  14 in total

1.  Chromogenic depsipeptide substrates for beta-lactamases and penicillin-sensitive DD-peptidases.

Authors:  M Adam; C Damblon; B Plaitin; L Christiaens; J M Frère
Journal:  Biochem J       Date:  1990-09-01       Impact factor: 3.857

Review 2.  Interaction of penicillin with the bacterial cell: penicillin-binding proteins and penicillin-sensitive enzymes.

Authors:  P M Blumberg; J L Strominger
Journal:  Bacteriol Rev       Date:  1974-09

3.  The exocellular DD-carboxypeptidase-endopeptidase from Streptomyces albus G. Purification and chemical properties.

Authors:  C Duez; J M Frère; F Geurts; J M Ghuysen; L Dierickx; L Delcambe
Journal:  Biochem J       Date:  1978-12-01       Impact factor: 3.857

4.  Molecular weight, amino acid composition and physicochemical properties of the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39.

Authors:  J M Frère; R Moreno; J M Ghuysen
Journal:  Biochem J       Date:  1974-10       Impact factor: 3.857

5.  The exocellular beta-lactamase of Streptomyces albus G. Purification, properties and comparison with the exocellular DD-carboxypeptidase.

Authors:  C Duez; J M Frère; D Klein; M Noël; J M Ghuysen; L Delcambe; L Dierickx
Journal:  Biochem J       Date:  1981-01-01       Impact factor: 3.857

6.  Kinetics of concomitant transfer and hydrolysis reactions catalysed by the exocellular DD-carboxypeptidase-transpeptidase of streptomyces R61.

Authors:  J M Frère; J M Ghuysen; H R Perkins; M Nieto
Journal:  Biochem J       Date:  1973-11       Impact factor: 3.857

7.  Fluorescence and circular dichroism studies on the Streptomyces R61 DD-carboxypeptidase-transpeptidase. Penicillin binding by the enzyme.

Authors:  M Nieto; H R Perkins; J M Frère; J M Ghuysen
Journal:  Biochem J       Date:  1973-11       Impact factor: 3.857

8.  Effects of nucleophiles on the breakdown of the benzylpenicilloyl-enzyme complex EI formed between benzylpenicillin and the exocellular DD-carboxypeptidase--transpeptiase of Streptomyces strain R61.

Authors:  A Marquet; J M Frère; J M Ghuysen; A Loffet
Journal:  Biochem J       Date:  1979-03-01       Impact factor: 3.857

9.  Delta 2- and delta 3-cephalosporins, penicillinate and 6-unsubstituted penems. Intrinsic reactivity and interaction with beta-lactamases and D-alanyl-D-alanine-cleaving serine peptidases.

Authors:  J M Frère; J A Kelly; D Klein; J M Ghuysen; P Claes; H Vanderhaeghe
Journal:  Biochem J       Date:  1982-04-01       Impact factor: 3.857

10.  Des-, syn- and anti-oxyimino-delta 3-cephalosporins. Intrinsic reactivity and reaction with RTEM-2 serine beta-lactamase and D-alanyl-D-alanine-cleaving serine and Zn2+-containing peptidases.

Authors:  G Laurent; F Durant; J M Frere; D Klein; J M Ghuysen
Journal:  Biochem J       Date:  1984-03-15       Impact factor: 3.857
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