Literature DB >> 4464852

Molecular weight, amino acid composition and physicochemical properties of the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39.

J M Frère, R Moreno, J M Ghuysen.   

Abstract

The exocellular dd-carboxypeptidase-transpeptidase from Streptomyces R39 was purified to protein homogeneity and in milligram amounts. The isolated enzyme consisted of one polypeptide chain of molecular weight about 53300. Its amino acid composition and several physicochemical properties were determined and compared with those of the exo-cellular dd-carboxypeptidase-transpeptidase from Streptomyces R61.

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Year:  1974        PMID： 4464852      PMCID： PMC1168371          DOI： 10.1042/bj1430233

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  14 in total

1.  Structure of the wall peptidoglycan of Streptomyces R39 and the specificity profile of its exocellular DD-carboxypeptidase--transpeptidase for peptide acceptors.

Authors:  J M Ghuysen; M Leyh-Bouille; J N Campbell; R Moreno; J M Frére; C Duez; M Nieto; H R Perkins
Journal:  Biochemistry       Date:  1973-03-27       Impact factor: 3.162

2.  Molecular weight and amino acid composition of the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61.

Authors:  J M Frère; J M Ghuysen; H R Perkins; M Nieto
Journal:  Biochem J       Date:  1973-11       Impact factor: 3.857

3.  Penicillin-sensitive DD-carboxypeptidases from Streptomyces strains R39 and K11.

Authors:  M Leyh-Bouille; M Nakel; J M Frère; K Johnson; J M Ghuysen; M Nieto; H R Perkins
Journal:  Biochemistry       Date:  1972-03-28       Impact factor: 3.162

4.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

5.  D-alanine carboxypeptidase from Bacillus subtilis membranes. I. Purification and characterization.

Authors:  J N Umbreit; J L Strominger
Journal:  J Biol Chem       Date:  1973-10-10       Impact factor: 5.157

6.  Penicillin-sensitive DD-carboxypeptidase from Streptomyces strain R 61.

Authors:  M Leyh-Bouille; J Coyette; J M Ghuysen; J Idczak; H R Perkins; M Nieto
Journal:  Biochemistry       Date:  1971-05-25       Impact factor: 3.162

7.  Binding of beta-lactam antibiotics to the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39.

Authors:  J M Frère; J M Ghuysen; P E Reynolds; R Moreno
Journal:  Biochem J       Date:  1974-10       Impact factor: 3.857

8.  Kinetics of concomitant transfer and hydrolysis reactions catalysed by the exocellular DD-carboxypeptidase-transpeptidase of streptomyces R61.

Authors:  J M Frère; J M Ghuysen; H R Perkins; M Nieto
Journal:  Biochem J       Date:  1973-11       Impact factor: 3.857

9.  Fluorescence and circular dichroism studies on the Streptomyces R61 DD-carboxypeptidase-transpeptidase. Penicillin binding by the enzyme.

Authors:  M Nieto; H R Perkins; J M Frère; J M Ghuysen
Journal:  Biochem J       Date:  1973-11       Impact factor: 3.857

10.  Isolation by covalent affinity chromatography of the penicillin-binding components from membranes of Bacillus subtilis.

Authors:  P M Blumberg; J L Strominger
Journal:  Proc Natl Acad Sci U S A       Date:  1972-12       Impact factor: 11.205
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  14 in total

1.  Accumulation of acyl-enzyme in DD-peptidase-catalysed reactions with analogues of peptide substrates.

Authors:  M Jamin; M Adam; C Damblon; L Christiaens; J M Frère
Journal:  Biochem J       Date:  1991-12-01       Impact factor: 3.857

2.  Chromogenic depsipeptide substrates for beta-lactamases and penicillin-sensitive DD-peptidases.

Authors:  M Adam; C Damblon; B Plaitin; L Christiaens; J M Frère
Journal:  Biochem J       Date:  1990-09-01       Impact factor: 3.857

3.  The diversity of the catalytic properties of class A beta-lactamases.

Authors:  A Matagne; A M Misselyn-Bauduin; B Joris; T Erpicum; B Granier; J M Frère
Journal:  Biochem J       Date:  1990-01-01       Impact factor: 3.857

4.  Kinetic study of interaction between BRL 42715, beta-lactamases, and D-alanyl-D-alanine peptidases.

Authors:  A Matagne; P Ledent; D Monnaie; A Felici; M Jamin; X Raquet; M Galleni; D Klein; I François; J M Frère
Journal:  Antimicrob Agents Chemother       Date:  1995-01       Impact factor: 5.191

5.  Mode of interaction between beta-lactam antibiotics and the exocellular DD-carboxypeptidase--transpeptidase from Streptomyces R39.

Authors:  N Fuad; J M Frère; J M Ghuysen; C Duez; M Iwatsubo
Journal:  Biochem J       Date:  1976-06-01       Impact factor: 3.857

6.  Enzymatic method for rapid and sensitive determination of beta-lactam antibiotics.

Authors:  J M Frère; D Klein; J M Ghuysen
Journal:  Antimicrob Agents Chemother       Date:  1980-10       Impact factor: 5.191

7.  Synthesis, purification and kinetic properties of fluorescein-labelled penicillins.

Authors:  B Lakaye; C Damblon; M Jamin; M Galleni; S Lepage; B Joris; J Marchand-Brynaert; C Frydrych; J M Frere
Journal:  Biochem J       Date:  1994-05-15       Impact factor: 3.857

8.  Binding of beta-lactam antibiotics to the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39.

Authors:  J M Frère; J M Ghuysen; P E Reynolds; R Moreno
Journal:  Biochem J       Date:  1974-10       Impact factor: 3.857

9.  Delta 2- and delta 3-cephalosporins, penicillinate and 6-unsubstituted penems. Intrinsic reactivity and interaction with beta-lactamases and D-alanyl-D-alanine-cleaving serine peptidases.

Authors:  J M Frère; J A Kelly; D Klein; J M Ghuysen; P Claes; H Vanderhaeghe
Journal:  Biochem J       Date:  1982-04-01       Impact factor: 3.857

10.  Simultaneous deletion of D-alanine carboxypeptidase IB-C and penicillin-binding component IV in a mutant of Escherichia coli K12.

Authors:  M Iwaya; J L Strominger
Journal:  Proc Natl Acad Sci U S A       Date:  1977-07       Impact factor: 11.205
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