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Literature DB >> 4630611

Inhibition of a DNA polymerase from Bacillus subtilis by hydroxyphenylazopyrimidines.

Abstract

The antimicrobial agent, 6-(p-hydroxyphenylazo)-uracil, specifically inhibits DNA polymerase III of Bacillus subtilis with a K(i) of less than 1 muM. The inhibition requires prior reduction of the drug, is reversible, and is competitive with dGTP. High amounts of dATP prevent inhibition by the closely related drug, 6-(p-hydroxyphenylazo)-isocytosine. A model is presented in which the inhibitors base-pair with the template while part of a ternary complex with the enzyme. These results imply that DNA polymerase III of B. subtilis is necessary for chromosomal replication.

Entities: Chemical Species

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Year: 1973 PMID： 4630611 PMCID： PMC433193 DOI： 10.1073/pnas.70.1.103

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

18 in total

1. ANTIMETABOLITES AFFECTING PROTEIN OR NUCLEIC ACID SYNTHESIS. PHLEOMYCIN, AN INHIBITOR OF DNA POLYMERASE.

Authors: A FALASCHI; A KORNBERG
Journal: Fed Proc Date: 1964 Sep-Oct

2. Altered deoxyribonucleic acid polymerase activity in a methyl methanesulfonate-sensitive mutant of Bacillus subtilis.

Authors: K B Gass; T C Hill; M Goulian; B S Strauss; N R Cozzarelli
Journal: J Bacteriol Date: 1971-10 Impact factor: 3.490

3. Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4.

Authors: M Goulian; Z J Lucas; A Kornberg
Journal: J Biol Chem Date: 1968-02-10 Impact factor: 5.157

4. Inhibition of the synthesis of deoxyribonucleic acid in bacteria by 6-(p-hydroxyphenylazo)-2,4-dihydroxypyrimidine. I. Metabolic studies in Streptococcus fecalis.

Authors: N C Brown; R E Handschumacher
Journal: J Biol Chem Date: 1966-07-10 Impact factor: 5.157

5. Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.

Authors: T M Jovin; P T Englund; L L Bertsch
Journal: J Biol Chem Date: 1969-06-10 Impact factor: 5.157

6. Inhibition of bacterial DNA replication by 6-(p-hydroxyphenylazo)-uracil: differential effect on repair and semi-conservative synthesis in Bacillus subtilis.

Authors: N C Brown
Journal: J Mol Biol Date: 1971-07-14 Impact factor: 5.469

7. 6-(p-hydroxyphenylazo)-uracil: a selective inhibitor of host DNA replication in phage-infected Bacillus subtilis.

Authors: N C Brown
Journal: Proc Natl Acad Sci U S A Date: 1970-11 Impact factor: 11.205

8. Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.

Authors: M L Gefter; Y Hirota; T Kornberg; J A Wechsler; C Barnoux
Journal: Proc Natl Acad Sci U S A Date: 1971-12 Impact factor: 11.205

9. Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II.

Authors: T Kornberg; M L Gefter
Journal: Proc Natl Acad Sci U S A Date: 1971-04 Impact factor: 11.205

10. A new DNA polymerase activity of Escherichia coli. I. Purification and properties of the activity present in E. coli polA1.

Authors: R E Moses; C C Richardson
Journal: Biochem Biophys Res Commun Date: 1970-12-24 Impact factor: 3.575

30 in total

Inhibition of a DNA polymerase from Bacillus subtilis by hydroxyphenylazopyrimidines.

1. ANTIMETABOLITES AFFECTING PROTEIN OR NUCLEIC ACID SYNTHESIS. PHLEOMYCIN, AN INHIBITOR OF DNA POLYMERASE.

2. Altered deoxyribonucleic acid polymerase activity in a methyl methanesulfonate-sensitive mutant of Bacillus subtilis.

3. Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4.

4. Inhibition of the synthesis of deoxyribonucleic acid in bacteria by 6-(p-hydroxyphenylazo)-2,4-dihydroxypyrimidine. I. Metabolic studies in Streptococcus fecalis.

5. Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.

6. Inhibition of bacterial DNA replication by 6-(p-hydroxyphenylazo)-uracil: differential effect on repair and semi-conservative synthesis in Bacillus subtilis.

7. 6-(p-hydroxyphenylazo)-uracil: a selective inhibitor of host DNA replication in phage-infected Bacillus subtilis.

8. Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.

9. Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II.

10. A new DNA polymerase activity of Escherichia coli. I. Purification and properties of the activity present in E. coli polA1.

1. Properties of a Bacillus subtilis strain lacking DNA polymerase I.

2. Induction of prophage SPO2 in Bacillus subtilis: isolation of excised prophage DNA as a covently closed circle.

3. Specific labelling of replicating SPP1 DNA: analysis of viral DNA synthesis and identification of phage DNA-genes.

4. Inhibition of bacteriophage PBS2 replication in Bacillus subtilis by phleomycin.

5. Induction of mutations in B. subtilis phage SPP1 by growth on host cells carrying a mutator DNA polymerase III.

6. A simplified procedure for the analysis of DNA polymerase III levels in Bacillus subtilis strains.

7. Effect of deoxyribonucleic acid replication inhibitors on bacterial recombination.

8. Mapping of the gene specifying DNA polymerase III of Bacillus subtilis.

9. Effect of lysogeny on transfection and transfection enhancement in Bacillus subtilis.

10. Dextran sulfates as a contaminant of DNA extracted from concentrated viruses and as an inhibitor of DNA polymerases.