Literature DB >> 357755

Dextran sulfates as a contaminant of DNA extracted from concentrated viruses and as an inhibitor of DNA polymerases.

R A Hitzeman, A M Hanel, A R Price.   

Abstract

Dextran sulfate is commonly used with polyethylene glycol to concentrate viruses before extraction of their DNA. However, dextran slulfate then easily contaminated such DNA and acted as a potent inhibitor of DNA polymerases from Bacillus subtilis (III), phage PBS2, and phage T4. Dextran sulfate only weakly inhibited Micrococcus luteus and Escherichia coli DNA polymerase I preparations.

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Year:  1978        PMID： 357755      PMCID： PMC354157     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  12 in total

1.  MECHANISM OF DEXTRAN SULFATE INHIBITION OF ATTENUATED POLIOVIRUS.

Authors:  S BENGTSSON
Journal:  Proc Soc Exp Biol Med       Date:  1965-01

2.  Practical procedures for the purification of bacterial viruses.

Authors:  U Bachrach; A Friedmann
Journal:  Appl Microbiol       Date:  1971-10

3.  Inhibition of polynucleotide kinase by agar, dextran sulfate and other polysaccharide sulfates.

Authors:  R Wu
Journal:  Biochem Biophys Res Commun       Date:  1971-05-21       Impact factor: 3.575

4.  Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.

Authors:  K R Yamamoto; B M Alberts; R Benzinger; L Lawhorne; G Treiber
Journal:  Virology       Date:  1970-03       Impact factor: 3.616

5.  Interactions between dextran sulfate and Escherichia coli ribosomes.

Authors:  F Miyazawa; O R Olijnyk; C J Tilley; T Tamaoki
Journal:  Biochim Biophys Acta       Date:  1967-08-22

6.  Effects of some inhibitors of protein synthesis on the binding of aminoacyl tRNA to ribosomal subunits.

Authors:  D Vazquez; R E Monro
Journal:  Biochim Biophys Acta       Date:  1967-06-20

7.  Bacillus subtilis DNA polymerases.

Authors:  K B Gass; N R Cozzarelli
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

8.  Control of DNA synthesis in Bacillus subtilis by phage phi e.

Authors:  M Marcus; M C Newlon
Journal:  Virology       Date:  1971-04       Impact factor: 3.616

9.  New deoxyribonucleic acid polymerase induced by Bacillus subtilis bacteriophage PBS2.

Authors:  A R Price; S J Cook
Journal:  J Virol       Date:  1972-04       Impact factor: 5.103

10.  Inhibition of a DNA polymerase from Bacillus subtilis by hydroxyphenylazopyrimidines.

Authors:  K B Gass; R L Low; N R Cozzarelli
Journal:  Proc Natl Acad Sci U S A       Date:  1973-01       Impact factor: 11.205
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  1 in total

1.  Dextran sodium sulfate inhibition of real-time polymerase chain reaction amplification: a poly-A purification solution.

Authors:  T A Kerr; M A Ciorba; H Matsumoto; V R T Davis; J Luo; S Kennedy; Y Xie; A Shaker; B K Dieckgraefe; N O Davidson
Journal:  Inflamm Bowel Dis       Date:  2011-05-25       Impact factor: 5.325
  1 in total

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