Literature DB >> 4943556

Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.

M L Gefter, Y Hirota, T Kornberg, J A Wechsler, C Barnoux.   

Abstract

A series of double mutants carrying one of the thermosensitive mutations for DNA synthesis (dnaA, B, C, D, E, F, and G) and the polA1 mutation of DeLucia and Cairns, were constructed. Enzyme activities of DNA Polymerases II and III were measured in each mutant. DNA Polymerase II activity was normal in all strains tested. DNA Polymerase III activity is thermosensitive specifically in those strains having thermoscnsitive mutations at the dnaE locus. From these results we conclude that DNA Polymerases II and III are independent enzymes and that DNA Polymerase III is an enzyme required for DNA replication in Escherichia coli.

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Year:  1971        PMID： 4943556      PMCID： PMC389610          DOI： 10.1073/pnas.68.12.3150

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  12 in total

1.  Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli.

Authors:  G BERTANI
Journal:  J Bacteriol       Date:  1951-09       Impact factor: 3.490

2.  On the process of cellular division in Escherichia coli. 3. Thermosensitive mutants of Escherichia coli altered in the process of DNA initiation.

Authors:  Y Hirota; J Mordoh; F Jacob
Journal:  J Mol Biol       Date:  1970-11-14       Impact factor: 5.469

3.  DNA polymerase II.

Authors:  R Knippers
Journal:  Nature       Date:  1970-12-12       Impact factor: 49.962

4.  Escherichia coli mutants with temperature-sensitive synthesis of DNA.

Authors:  P L Carl
Journal:  Mol Gen Genet       Date:  1970

5.  Isolation of an E. coli strain with a mutation affecting DNA polymerase.

Authors:  P De Lucia; J Cairns
Journal:  Nature       Date:  1969-12-20       Impact factor: 49.962

6.  DNA transfer and DNA synthesis during bacterial conjugation.

Authors:  F Bonhoeffer
Journal:  Z Vererbungsl       Date:  1966

7.  Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division.

Authors:  Y Hirota; A Ryter; F Jacob
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1968

8.  [Thermosensitive mutants of Escherichia coli K 12. I. Isolation and rapid characterization].

Authors:  M Kohiyama; D Cousin; A Ryter; F Jacob
Journal:  Ann Inst Pasteur (Paris)       Date:  1966-04

9.  On the process of cellular division in Escherichia coli. V. Incorporation of deoxynucleoside triphosphates by DNA thermosensitive mutants of Escherichia coli also lacking DNA polymerase activity.

Authors:  J Mordoh; Y Hirota; F Jacob
Journal:  Proc Natl Acad Sci U S A       Date:  1970-10       Impact factor: 11.205

10.  A new DNA polymerase acitvity of Escherichia coli. II. Properties of the enzyme purified from wild-type E. coli and DNA-ts mutants.

Authors:  R E Moses; C C Richardson
Journal:  Biochem Biophys Res Commun       Date:  1970-12-24       Impact factor: 3.575
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  156 in total

1.  Conditional-lethal mutations that suppress genetic defects in morphogenesis by altering structural proteins.

Authors:  J Jarvik; D Botstein
Journal:  Proc Natl Acad Sci U S A       Date:  1975-07       Impact factor: 11.205

Review 2.  Colicinogeny and related phenomena.

Authors:  K G Hardy
Journal:  Bacteriol Rev       Date:  1975-12

3.  Interference of dna ts mutations of Escherichia coli with thymineless death.

Authors:  F Bouvier; N Sicard
Journal:  J Bacteriol       Date:  1975-12       Impact factor: 3.490

4.  DNA synthesis involving a complexes form of DNA polymerase I in extracts of Escherichia coli.

Authors:  R W Hendler; M Pereira; R Scharff
Journal:  Proc Natl Acad Sci U S A       Date:  1975-06       Impact factor: 11.205

5.  Partial suppression of the phenotype of Escherichia coli K-12 dnaG mutants by some I-like conjugative plasmids.

Authors:  B M Wilkins
Journal:  J Bacteriol       Date:  1975-06       Impact factor: 3.490

6.  Synthesis of complex forms of bacteriophage phiX174 double-stranded DNA in a temperature-sensitive dnaC mutant of Escherichia coli C.

Authors:  E G Kranias; L B Dumas
Journal:  J Virol       Date:  1975-08       Impact factor: 5.103

Review 7.  Handoff from recombinase to replisome: insights from transposition.

Authors:  H Nakai; V Doseeva; J M Jones
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-17       Impact factor: 11.205

8.  Association of phiX174 DNA-dependent ATPase activity with an Escherichia coli protein, replication factor Y, required for in vitro synthesis of phiX174 DNA.

Authors:  S Wickner; J Hurwitz
Journal:  Proc Natl Acad Sci U S A       Date:  1975-09       Impact factor: 11.205

9.  Bacillus subtilis dnaF: a mutation of the gene specifying the structure of DNA polymerase III.

Authors:  M J Vrooman; M H Barnes; N C Brown
Journal:  Mol Gen Genet       Date:  1978-09-08

10.  Selective inhibition of nuclear DNA synthesis by 9- -D-arabinofuranosyl adenine in rat cells transformed by Rous sarcoma virus.

Authors:  C Shipman; S H Smith; J C Drach
Journal:  Proc Natl Acad Sci U S A       Date:  1972-07       Impact factor: 11.205
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