Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Proteins at the tRNA binding sites of Escherichia coli ribosomes.

Literature DB >> 4589893

Proteins at the tRNA binding sites of Escherichia coli ribosomes.

A P Czernilofsky, E E Collatz, G Stöffler, E Kuechler.

Abstract

p - Nitrophenylcarbamyl - phenylalanyl-tRNA binds to ribosomes in response to poly(U) and reacts with ribosomal proteins via covalent bond formation. Ribosomal proteins of Escherichia coli were characterized by two-dimensional gel electrophoresis and by reaction with specific antibodies. The affinity label is shown to react with the 50S proteins L27, L15, L2, L16, and L14. We therefore conclude that these particular proteins are located at or near tRNA-binding sites within the 50S ribosomal subunit.

Entities: Chemical Species

Mesh：

Substances：

Year: 1974 PMID： 4589893 PMCID： PMC387971 DOI： 10.1073/pnas.71.1.230

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

23 in total

1. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

Authors: R G MARTIN; B N AMES
Journal: J Biol Chem Date: 1961-05 Impact factor: 5.157

2. Affinity label for the tRNA binding site on the Escherichia coli ribosome.

Authors: A P Czernilofsky; E Kuechler
Journal: Biochim Biophys Acta Date: 1972-07-31

3. Ribosomal protein neighborhoods. I. S18 and S21 as well as S5 and S8 are neighbors.

Authors: L C Lutter; H Zeichhardt; C G Kurland
Journal: Mol Gen Genet Date: 1972

4. Assembly mapping of 30S ribosomal proteins from E. coli.

Authors: S Mizushima; M Nomura
Journal: Nature Date: 1970-06-27 Impact factor: 49.962

5. Polypeptide chain initiation: nucleotide sequences of the three ribosomal binding sites in bacteriophage R17 RNA.

Authors: J A Steitz
Journal: Nature Date: 1969-12-06 Impact factor: 49.962

6. Studies on the formation of transfer ribonucleic acid-ribosome complexes. 8. Aminoacyl oligonucleotide binding to ribosomes: characteristics and requirements.

Authors: S Pestka; T Hishizawa; J L Lessard
Journal: J Biol Chem Date: 1970-11-25 Impact factor: 5.157

7. Sequence differences of Escherichia coli 30S ribosomal proteins as determined by immunochemical methods.

Authors: G Stöffler; H G Wittmann
Journal: Proc Natl Acad Sci U S A Date: 1971-09 Impact factor: 11.205

8. Studies on the formation of transfer ribonucleic acid-ribosome complexes. XI. Antibiotic effects on phenylalanyl-oligonucleotide binding to ribosomes.

Authors: S Pestka
Journal: Proc Natl Acad Sci U S A Date: 1969-10 Impact factor: 11.205

8. Identification of components of the streptomycin-binding center of E. coli MRE 600 ribosomes by photo-affinity labelling.

Authors: A S Girshovich; E S Bochkareva; Y A Ovchinnikov
Journal: Mol Gen Genet Date: 1976-03-22

9. Mutational study of the rRNA in yeast mitochondria: functional importance of T1696 in the large rRNA gene.

Authors: B Daignan-Fornier; M Bolotin-Fukuhara
Journal: Nucleic Acids Res Date: 1988-10-11 Impact factor: 16.971

10. Affinity labelling of yeast ribosomal peptidyl transferase.

Authors: M Pérez-Gosálbez; D Vázquez; J P Ballesta
Journal: Mol Gen Genet Date: 1978-07-06

Proteins at the tRNA binding sites of Escherichia coli ribosomes.

1. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

2. Affinity label for the tRNA binding site on the Escherichia coli ribosome.

3. Ribosomal protein neighborhoods. I. S18 and S21 as well as S5 and S8 are neighbors.

4. Assembly mapping of 30S ribosomal proteins from E. coli.

5. Polypeptide chain initiation: nucleotide sequences of the three ribosomal binding sites in bacteriophage R17 RNA.

6. Studies on the formation of transfer ribonucleic acid-ribosome complexes. 8. Aminoacyl oligonucleotide binding to ribosomes: characteristics and requirements.

7. Sequence differences of Escherichia coli 30S ribosomal proteins as determined by immunochemical methods.

8. Studies on the formation of transfer ribonucleic acid-ribosome complexes. XI. Antibiotic effects on phenylalanyl-oligonucleotide binding to ribosomes.

9. Three-dimensional structure of yeast phenylalanine transfer RNA: folding of the polynucleotide chain.

10. Identification of the chloramphenicol-binding protein in Escherichia coli ribosomes by partial reconstitution.

1. Identification of the 30 S protein adjacent to peptidyl transferase catalytic center of Escherichia coli ribosomes.

2. Identification of a ribosomal protein essential for peptidyl transferase activity.

3. Analysis of lincomycin resistance mutations in Escherichia coli.

4. Analogs of methionyl-tRNA synthetase substrates containing photolabile groups.

5. A kinase that transfers the gamma-phosphoryl group of GTP to proteins of eukaryotic 40S ribosomal subunits.

6. Affinity labeling of the ribosomal decoding site with an AUG-substrate analog.

7. Photoincorporation of puromycin and N-(ethyl-2-diazomalonyl)puromycin into Escherichia coli ribosomes.

8. Identification of components of the streptomycin-binding center of E. coli MRE 600 ribosomes by photo-affinity labelling.

9. Mutational study of the rRNA in yeast mitochondria: functional importance of T1696 in the large rRNA gene.

10. Affinity labelling of yeast ribosomal peptidyl transferase.