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Literature DB >> 1055382

Identification of a ribosomal protein essential for peptidyl transferase activity.

V G Moore, R E Atchison, G Thomas, M Moran, H F Noller.

Abstract

Extraction with 2 M lithium chloride removes a group of proteins (LiC1 SP) from 50S ribosomal subunits. Both the LiC1 SP and the resulting cores, which contain the remaining proteins as well as 5S and 23S RNA, lack peptidyl transferase activity, as measured by the "fragment reaction". Activity can be restored to the LiC1 cores by reconstitution with LiC1 SP under conditions of high temperature and high ionic strength. The LiC1 SP proteins were fractionated by carboxymethyl-cellulose and Sephadex G-100, and the individual fractions were tested by this reconstitution system. Of the 18 ribosomal proteins found in the LiC1 SP, only L16 is essential for reconstitution of peptidyl transferase activity.

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Year: 1975 PMID： 1055382 PMCID： PMC432417 DOI： 10.1073/pnas.72.3.844

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

24 in total

7. Binding of chloramphenicol and a fragment of aminoacyl-transfer ribonucleic acid to ribosomes and a ribosome precursor from a mutant of Escherichia coli.

Authors: P D Butler; P F Sims; D G Wild
Journal: Biochem J Date: 1978-06-15 Impact factor: 3.857

Identification of a ribosomal protein essential for peptidyl transferase activity.

1. THE PUROMYCIN REACTION AND ITS RELATION TO PROTEIN SYNTHESIS.

2. E. coli 30 S and 50 S ribosomal subparticle components in the localization region of the tRNA acceptor terminus.

3. Affinity labelling of ribosomal peptidyl transferase by a puromycin analogue.

4. A protein involved in the peptidyltransferase activity of Escherichia coli ribosomes.

5. Irreversible binding of N-iodoacetylpuromycin to E. coli ribosomes.

6. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.

7. Peptidyl-transferase activity of Escherichia coli ribosomes digested by ribonuclease T 1 .

8. The peptidyl transferase activity of ribosomes.

9. Identification of 50S proteins at the peptidyl-tRNA binding site of Escherichia coli ribosomes.

10. Identification of the chloramphenicol-binding protein in Escherichia coli ribosomes by partial reconstitution.

1. Thiostrepton-resistant mutants exhibit relaxed synthesis of RNA.

2. Evolution of protein synthesis from an RNA world.

3. The sarcin-ricin loop of 23S rRNA is essential for assembly of the functional core of the 50S ribosomal subunit.

Review 4. What recent ribosome structures have revealed about the mechanism of translation.

Review 5. Peptidyl transferase: protein, ribonucleoprotein, or RNA?

Review 6. The parable of the caveman and the Ferrari: protein synthesis and the RNA world.

7. Binding of chloramphenicol and a fragment of aminoacyl-transfer ribonucleic acid to ribosomes and a ribosome precursor from a mutant of Escherichia coli.

8. Characterization of methylated neutral amino acids from Escherichia coli ribosomes.

9. The composition of an unusual precursor of 50 S ribosomes in a mutant of Escherichia coli.

10. Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome.