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Literature DB >> 1093183

Affinity labeling of the ribosomal decoding site with an AUG-substrate analog.

Abstract

The trinucleotide AUG was condensed at the 5'-end with N-bromoacetyl-p-aminophenylphosphate. This bromoactylated AUG analog reacted irreversibly with the mRNA binding site of Escherichia coli 70S ribosomes. After reaction of 70S ribosomes with the AUG analog, labeled 30S subunits could be isolated that were programmed for initiation-factor-dependent binding of fMet-tRNAfMet. This shows that this AUG-affinity label reacted in the decoding site for fMet-tRNAfMet. By combination of sodium dodecyl sulfate-, Sarkosyl-, and ureapolyacrylamide gel electrophoresis the AUG-affinity label was found to be irreversibly bound to ribosomal proteins S4, the ram gene product, and S18.

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Year: 1975 PMID： 1093183 PMCID： PMC432565 DOI： 10.1073/pnas.72.4.1505

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

21 in total

Review 1. Assembly of bacterial ribosomes.

Authors: M Nomura
Journal: Science Date: 1973-03-02 Impact factor: 47.728

2. Electrophoretic and immunological studies on ribosomal proteins of 100 Escherichia coli revertants from streptomycin dependence.

Authors: R Hasenbank; C Guthrie; G Stöffler; H G Wittmann; L Rosen; D Apirion
Journal: Mol Gen Genet Date: 1973-12-14

3. Proteins at the tRNA binding sites of Escherichia coli ribosomes.

Authors: A P Czernilofsky; E E Collatz; G Stöffler; E Kuechler
Journal: Proc Natl Acad Sci U S A Date: 1974-01 Impact factor: 11.205

4. An improved technique for the analysis of ribonucleoprotein fragments from Escherichia coli 30S ribosomes.

Authors: R Brimacombe; J M Morgan; R A Cox
Journal: Eur J Biochem Date: 1971-11-11

5. Letter: Inhibition of phenylalanyl-tRNA binding and elongation factor Tu-dependent GTP hydrolysis by antibodies specific for several ribosomal proteins.

Authors: J H Highland; E Ochsner; J Gordon; R Hasenbank; G Stöffler
Journal: J Mol Biol Date: 1974-06-15 Impact factor: 5.469

6. Ribosomal proteins. XII. Number of proteins in small and large ribosomal subunits of Escherichia coli as determined by two-dimensional gel electrophoresis.

Authors: E Kaltschmidt; H G Wittmann
Journal: Proc Natl Acad Sci U S A Date: 1970-11 Impact factor: 11.205

1. Location of protein S1 of Escherichia coli ribosomes at the 'A'-site of the codon binding site. Affinity labeling studies with a 3'-modified A-U-G analog.

Authors: O Pongs; G Stöffler; R W Bald
Journal: Nucleic Acids Res Date: 1976-07 Impact factor: 16.971

2. Comparison of the reactions of chemically reactive analogs of U-G-A and of A-U-G with ribosomes of Escherichia coli.

Authors: O Pongs; E Rossner
Journal: Nucleic Acids Res Date: 1976-07 Impact factor: 16.971

3. Affinity labelling of rat liver ribosomal protein S26 by heptauridylate containing a 5'-terminal alkylating group.

Authors: J Stahl; N D Kobetz
Journal: Mol Biol Rep Date: 1984-01 Impact factor: 2.316

3 in total