Microfermentation series for identification of single colonies of Enterobacteriaceae.

Microfermentation tests for members of the family Enterobacteriaceae were devised by using agar solutions in disposable, multi-welled, plastic trays. The tests could be made directly from isolated colonies picked from agar plates and represented a considerable saving in time, labor, and materials over the conventional methods. Tests were formulated for determining carbohydrate fermentations, citrate utilization, motility, amino acid decarboxylation, and production of H(2)S, indole, urease, and acetyl-methyl-carbinol.