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Literature DB >> 4530982

Tubulin synthesis on polysomes isolated from brain and leg muscle of embryonic chick.

Abstract

A protein-synthesizing system has been established in vitro, in which tubulin is synthesized on polysomes isolated from brain and leg muscle of embryonic chick. The tubulin synthesized in vitro is characterized by (i) its electrophoretic mobility on sodium dodecyl sulfate-acrylamide gels (brain: 55,000 daltons; leg muscle: 53,000 daltons), and (ii) its ability to function as a microtubular subunit, as judged by its specific ability to participate in at least two polymerization and depolymerization steps in the microtubule assembly system in vitro.

Entities: Chemical Disease

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Year: 1974 PMID： 4530982 PMCID： PMC433865 DOI： 10.1073/pnas.71.11.4278

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

15 in total

1. The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.

Authors: J A Spudich; S Watt
Journal: J Biol Chem Date: 1971-08-10 Impact factor: 5.157

10. Chlamydomonas flagella. I. Isolation and electrophoretic analysis of microtubules, matrix, membranes, and mastigonemes.

Authors: G B Witman; K Carlson; J Berliner; J L Rosenbaum
Journal: J Cell Biol Date: 1972-09 Impact factor: 10.539

1 in total

1. Differential binding of methyl benzimidazol-2-yl carbamate to fungal tubulin as a mechanism of resistance to this antimitotic agent in mutant strains of Aspergillus nidulans.

Authors: L C Davidse; W Flach
Journal: J Cell Biol Date: 1977-01 Impact factor: 10.539

1 in total

Tubulin synthesis on polysomes isolated from brain and leg muscle of embryonic chick.

1. The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.

2. Nucleated assembly of microtubules in porcine brain extracts.

3. Myosin synthesis and specificity of eukaryotic initiation factors.

4. A study of muscle polyribosomes and the coprecipitation of polyribosomes with myosin.

5. The colchicine-binding protein of mammalian brain and its relation to microtubules.

6. Comparison of the microtubule proteins of neuroblastoma cells, brain, and Chlamydomonas flagella.

7. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

8. Microtubule formation in vitro in solutions containing low calcium concentrations.

9. Rat brain microtubule protein: purification and determination of covalently bound phosphate and carbohydrate.

10. Chlamydomonas flagella. I. Isolation and electrophoretic analysis of microtubules, matrix, membranes, and mastigonemes.

1. Differential binding of methyl benzimidazol-2-yl carbamate to fungal tubulin as a mechanism of resistance to this antimitotic agent in mutant strains of Aspergillus nidulans.