Literature DB >> 5289385

Comparison of the microtubule proteins of neuroblastoma cells, brain, and Chlamydomonas flagella.

J B Olmsted, G B Witman, K Carlson, J L Rosenbaum.   

Abstract

Intact A microtubules isolated from outer doublet microtubules of Chlamydomonas flagella contain two separable proteins (tubulins) that differ in molecular weight and in amino-acid composition. The microtubule protein isolated from brain or neuroblastoma cells also has two electrophoretically distinct tubulins. Although the two tubulins of brain and neuroblastoma cells are electrophoretically similar to each other, only one of these tubulins migrates with the flagellar tubulins. This is the first evidence that (a) isolated, morphologically intact, single microtubules from flagella contain at least two different tubulins, and (b) at least one of these tubulins differs from tubulins that are isolated from other sources.

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Year:  1971        PMID： 5289385      PMCID： PMC389399          DOI： 10.1073/pnas.68.9.2273

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  18 in total

1.  Nutritional studies with Chlamydomonas reinhardi.

Authors:  R SAGER; S GRANICK
Journal:  Ann N Y Acad Sci       Date:  1953-10-14       Impact factor: 5.691

2.  Simple method for quantitive densitometry of polyacrylamide gels using fast green.

Authors:  M A Gorovsky; K Carlson; J L Rosenbaum
Journal:  Anal Biochem       Date:  1970-06       Impact factor: 3.365

3.  Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.

Authors:  A L Shapiro; E Viñuela; J V Maizel
Journal:  Biochem Biophys Res Commun       Date:  1967-09-07       Impact factor: 3.575

4.  Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis.

Authors:  J L Hedrick; A J Smith
Journal:  Arch Biochem Biophys       Date:  1968-07       Impact factor: 4.013

5.  Cytoplasmic filaments and tubules.

Authors:  M R Adelman; G G Borisy; M L Shelanski; R C Weisenberg; E W Taylor
Journal:  Fed Proc       Date:  1968 Sep-Oct

6.  Differential effects of antimitotic agents on the stability and behavior of cytoplasmic and ciliary microtubules.

Authors:  L G Tilney; J R Gibbins
Journal:  Protoplasma       Date:  1968       Impact factor: 3.356

7.  The colchicine-binding protein of mammalian brain and its relation to microtubules.

Authors:  R C Weisenberg; G G Borisy; E W Taylor
Journal:  Biochemistry       Date:  1968-12       Impact factor: 3.162

8.  An improvement in a procedure for counting tritium and carbon-14 in polyacrylamide gels.

Authors:  M Zaitlin; V Hariharasubramanian
Journal:  Anal Biochem       Date:  1970-05       Impact factor: 3.365

9.  Identification of the flagellar proteins of Chlamydomonas reinhardii.

Authors:  M Jacobs; A McVittie
Journal:  Exp Cell Res       Date:  1970-11       Impact factor: 3.905

10.  Flagellar elongation and shortening in Chlamydomonas. The use of cycloheximide and colchicine to study the synthesis and assembly of flagellar proteins.

Authors:  J L Rosenbaum; J E Moulder; D L Ringo
Journal:  J Cell Biol       Date:  1969-05       Impact factor: 10.539
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  29 in total

1.  Assembly of chick brain tubulin onto flagellar microtubules from Chlamydomonas and sea urchin sperm.

Authors:  L I Binder; W L Dentler; J L Rosenbaum
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205

2.  Spermatogenesis revisited. IV. Abnormal spermiogenesis in mice homozygous for another male-sterility-inducing mutation, hpy (hydrocephalic-polydactyl).

Authors:  J H Bryan
Journal:  Cell Tissue Res       Date:  1977-05-16       Impact factor: 5.249

3.  Microtubule structure at low resolution by x-ray diffraction.

Authors:  E Mandelkow; J Thomas; C Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205

4.  Identification of a protein related to tubulin in the postsynaptic density.

Authors:  H Feit; P Kelly; C W Cotman
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205

5.  The possible significance of adenosine 3':5'-cyclic monophosphate-stimulated protein kinase activity associated with purified microtubular protein preparations from mammalian brain.

Authors:  J R Lagnado; C A Lyons; M Weller; O Phillipson
Journal:  Biochem J       Date:  1972-07       Impact factor: 3.857

6.  Directionality of brain microtubule assembly in vitro.

Authors:  W L Dentler; S Granett; G B Witman; J L Rosenbaum
Journal:  Proc Natl Acad Sci U S A       Date:  1974-05       Impact factor: 11.205

7.  Effect of nerve growth factor on the expression of colchicine-binding activity and 14-3-2 protein in an established line of human neuroblastoma.

Authors:  A R Kolber; M N Goldstein; B W Moore
Journal:  Proc Natl Acad Sci U S A       Date:  1974-10       Impact factor: 11.205

8.  Tubulin synthesis on polysomes isolated from brain and leg muscle of embryonic chick.

Authors:  A O Jorgensen; S M Heywood
Journal:  Proc Natl Acad Sci U S A       Date:  1974-11       Impact factor: 11.205

9.  Adenosine 3',5'-cyclic monophosphate in Chlamydomonas reinhardtii. Influence on flagellar function and regeneration.

Authors:  R W Rubin; P Filner
Journal:  J Cell Biol       Date:  1973-03       Impact factor: 10.539

10.  Rat brain microtubule protein: purification and determination of covalently bound phosphate and carbohydrate.

Authors:  B A Eipper
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205
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