Literature DB >> 4522295

Altered enzymes in drug-resistant variants of mammalian tissue culture cells.

J D Sharp, N E Capecchi, M R Capecchi.   

Abstract

Two selective procedures are compared in an effort to isolate variants of mouse L cells containing structural gene mutations. Among the resulting variant cloned cell lines are found two types of alterations in the enzyme hypoxanthine phosphoribosyl transferase (EC 2.4.2.8) (1): enzyme with altered kinetic constants causing in vivo and in vitro resistance to 8-azaguanine; and (2) enzyme with altered heat sensitivity in vitro. These results support the view that tissue culture cell variants can arise from structural gene mutations.

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Year:  1973        PMID： 4522295      PMCID： PMC427189          DOI： 10.1073/pnas.70.11.3145

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  19 in total

1.  Some aspects of somatic cell hybridization.

Authors:  J W Littlefield; S Goldstein
Journal:  In Vitro       Date:  1970 Jul-Aug

2.  Isolation and characterization of thymidine transport mutants of Chinese hamster cells.

Authors:  R E Breslow; R A Goldsby
Journal:  Exp Cell Res       Date:  1969-06       Impact factor: 3.905

Review 3.  The statistical analysis of enzyme kinetic data.

Authors:  W W Cleland
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1967

4.  Micro-scale peptide mapping method for identification of variant proteins.

Authors:  S Watanabe; A Yoshida
Journal:  Biochem Genet       Date:  1971-12       Impact factor: 1.890

5.  Mutation rates in cells at different ploidy levels.

Authors:  M Harris
Journal:  J Cell Physiol       Date:  1971-10       Impact factor: 6.384

6.  Evidence for intergenic complementation in hybrid cells derived from two human diploid strains each carrying an X-linked mutation.

Authors:  M Siniscalco; H P Klinger; H Eagle; H Koprowski; W Y Fujimoto; J E Seegmiller
Journal:  Proc Natl Acad Sci U S A       Date:  1969-03       Impact factor: 11.205

7.  Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.

Authors:  F T Kao; T T Puck
Journal:  Proc Natl Acad Sci U S A       Date:  1968-08       Impact factor: 11.205

8.  Mammalian cell genetics. II. Chemical induction of specific locus mutations in Chinese hamster cells in vitro.

Authors:  E H Chu; H V Malling
Journal:  Proc Natl Acad Sci U S A       Date:  1968-12       Impact factor: 11.205

9.  Temperature sensitive conditional mutants of monkey kidney cells.

Authors:  P M Naha
Journal:  Nature       Date:  1969-09-27       Impact factor: 49.962

10.  Enzyme defect associated with a sex-linked human neurological disorder and excessive purine synthesis.

Authors:  J E Seegmiller; F M Rosenbloom; W N Kelley
Journal:  Science       Date:  1967-03-31       Impact factor: 47.728
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  36 in total

1.  Heterogeneity in the rate of benzo[a]pyrene metabolism in single cells: quantitation using flow cytometry.

Authors:  A G Miller; J P Whitlock
Journal:  Mol Cell Biol       Date:  1982-06       Impact factor: 4.272

2.  Density dependent inhibition of both growth and T-antigen expression in revertants isolated from simian virus 40-transformed mouse SVT2 cells.

Authors:  E G Gurney; T Gurney
Journal:  J Virol       Date:  1979-11       Impact factor: 5.103

3.  Properties of cells carrying the herpes simplex virus type 2 thymidine kinase gene: mechanisms of reversion to a thymidine kinase-negative phenotype.

Authors:  K F Bastow; G Darby; P Wildy; A C Minson
Journal:  J Virol       Date:  1980-12       Impact factor: 5.103

4.  Cotransfer of thymidine kinase and galactokinase genes by chromosome-mediated gene transfer.

Authors:  O W McBride; J W Burch; F H Ruddle
Journal:  Proc Natl Acad Sci U S A       Date:  1978-02       Impact factor: 11.205

5.  Identification of amber and ochre mutants of the human virus Ad2+ND1.

Authors:  R F Gesteland; N Wills; J B Lewis; T Grodzicker
Journal:  Proc Natl Acad Sci U S A       Date:  1977-10       Impact factor: 11.205

6.  Clonal variation in albumin messenger RNA activity in hepatoma cells.

Authors:  J A Peterson
Journal:  Proc Natl Acad Sci U S A       Date:  1976-06       Impact factor: 11.205

7.  Cloned cDNA sequences of the hypoxanthine/guanine phosphoribosyltransferase gene from a mouse neuroblastoma cell line found to have amplified genomic sequences.

Authors:  J Brennand; A C Chinault; D S Konecki; D W Melton; C T Caskey
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

8.  Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants.

Authors:  D S Konecki; J Brennand; J C Fuscoe; C T Caskey; A C Chinault
Journal:  Nucleic Acids Res       Date:  1982-11-11       Impact factor: 16.971

9.  Amplification of hypoxanthine-guanine phosphoribosyltransferase genes in chromosome-mediated gene transferents.

Authors:  S Linder; A W Coleman; J M Eisenstadt
Journal:  Mol Cell Biol       Date:  1984-04       Impact factor: 4.272

10.  Expression of purine overproduction in a series of 8-azaguanine-resistant diploid human lymphoblast lines.

Authors:  J E Lever; G Nuki; J E Seegmiller
Journal:  Proc Natl Acad Sci U S A       Date:  1974-07       Impact factor: 11.205
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