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Literature DB >> 6294614

Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants.

D S Konecki, J Brennand, J C Fuscoe, C T Caskey, A C Chinault.

Abstract

Recombinant plasmids containing DNA inserts complementary to mRNA coding for hypoxanthine-guanine phosphoribosyltransferase (HPRT) from mouse and Chinese hamster cell lines have been isolated from cDNA libraries and characterized by DNA sequence analysis. A total of 1292 nucleotides of the mouse cDNA sequence and 1301 nucleotides of the Chinese hamster cDNA sequence has been determined. Each of these sequences includes an open reading frame of 654 nucleotides (218 amino acids) corresponding to the HPRT protein coding region. The deduced amino acid sequences for the mouse and Chinese hamster enzymes are presented and compared to that of human HPRT. At least 95% of the amino acids are conserved in the three species. In addition, we present evidence that two species of HPRT mRNA, which differ in the site of polyadenylation that is utilized during processing of the RNA transcripts, exist in Chinese hamster cells.

Entities: Gene Species

Mesh：

Substances：

Year: 1982 PMID： 6294614 PMCID： PMC326962 DOI： 10.1093/nar/10.21.6763

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

28 in total

1. The isolation of a suppressible nonsense mutant in mammalian cells.

Authors: M R Capecchi; R A Haar; N E Capecchi; M M Sveda
Journal: Cell Date: 1977-10 Impact factor: 41.582

2. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

3. Altered enzymes in drug-resistant variants of mammalian tissue culture cells.

Authors: J D Sharp; N E Capecchi; M R Capecchi
Journal: Proc Natl Acad Sci U S A Date: 1973-11 Impact factor: 11.205

4. 3' non-coding region sequences in eukaryotic messenger RNA.

Authors: N J Proudfoot; G G Brownlee
Journal: Nature Date: 1976-09-16 Impact factor: 49.962

5. Enzymatic in vitro synthesis of globin genes.

Authors: A Efstratiadis; F C Kafatos; A M Maxam; T Maniatis
Journal: Cell Date: 1976-02 Impact factor: 41.582

6. Mutant chinese hamster cells with a thermosensitive hypoxanthine-guanine phosphoribosyltransferase.

Authors: R G Fenwick; C T Caskey
Journal: Cell Date: 1975-06 Impact factor: 41.582

7. Immunological characterization of hypoxanthine-guanine phosphoribosyl transferase mutants of mouse L cells: evidence for mutations at different loci in the HGPRT gene.

Authors: G M Wahl; S H Hughes; M R Capecchi
Journal: J Cell Physiol Date: 1975-04 Impact factor: 6.384

8. Analysis of HeLa cell hypoxanthine phosphoribosyltransferase mutants and revertants by two-dimensional polyacrylamide gel electrophoresis: evidence for silent gene activation.

Authors: G Milman; E Lee; G S Ghangas; J R McLaughlin; M George
Journal: Proc Natl Acad Sci U S A Date: 1976-12 Impact factor: 11.205

9. Mutations affecting the antigenic properties of hypoxanthine-guanine phosphoribosyl transferase in cultured Chinese hamster cells.

Authors: R G Fenwick; J J Wasmuth; C T Caskey
Journal: Somatic Cell Genet Date: 1977-03

10. Tryptic peptide analysis of normal and mutant forms of hypoxanthine phosphoribosyltransferase from HeLa cells.

Authors: G Milman; S W Krauss; A S Olsen
Journal: Proc Natl Acad Sci U S A Date: 1977-03 Impact factor: 11.205

59 in total

1. Random monoallelic expression of three genes clustered within 60 kb of mouse t complex genomic DNA.

Authors: Y Sano; T Shimada; H Nakashima; R H Nicholson; J F Eliason; T A Kocarek; M S Ko
Journal: Genome Res Date: 2001-11 Impact factor: 9.043

2. Increased rate of base substitution in a hamster mutator strain obtained during serial selection for gene amplification.

Authors: M A Caligo; W Armstrong; B J Rossiter; M Meuth
Journal: Mol Cell Biol Date: 1990-12 Impact factor: 4.272

10. Altered turnover of hypoxanthine phosphoribosyltransferase in erythroid cells of mice expressing Hprt a and Hprt b alleles.

Authors: G G Johnson; V M Chapman
Journal: Genetics Date: 1987-06 Impact factor: 4.562

Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants.

1. The isolation of a suppressible nonsense mutant in mammalian cells.

2. A new method for sequencing DNA.

3. Altered enzymes in drug-resistant variants of mammalian tissue culture cells.

4. 3' non-coding region sequences in eukaryotic messenger RNA.

5. Enzymatic in vitro synthesis of globin genes.

6. Mutant chinese hamster cells with a thermosensitive hypoxanthine-guanine phosphoribosyltransferase.

7. Immunological characterization of hypoxanthine-guanine phosphoribosyl transferase mutants of mouse L cells: evidence for mutations at different loci in the HGPRT gene.

8. Analysis of HeLa cell hypoxanthine phosphoribosyltransferase mutants and revertants by two-dimensional polyacrylamide gel electrophoresis: evidence for silent gene activation.

9. Mutations affecting the antigenic properties of hypoxanthine-guanine phosphoribosyl transferase in cultured Chinese hamster cells.

10. Tryptic peptide analysis of normal and mutant forms of hypoxanthine phosphoribosyltransferase from HeLa cells.

1. Random monoallelic expression of three genes clustered within 60 kb of mouse t complex genomic DNA.

2. Increased rate of base substitution in a hamster mutator strain obtained during serial selection for gene amplification.

3. Nucleotide sequence of cDNA clone for hypoxanthine-guanine phosphoribosyltransferase from Plasmodium falciparum.

4. Decreased blood pressure response in mice deficient of the alpha1b-adrenergic receptor.

5. Full-length cDNA sequence of the X-linked HPRT gene of an Australian marsupial, the wallaroo (Macropus robustus).

6. Studies on the mechanism of 3-deazaguanine cytotoxicity in L1210-sensitive and -resistant cell lines.

7. Coordinate control and variation in X-linked gene expression among female mice.

8. Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene.

9. Differential methylation of hypoxanthine phosphoribosyltransferase genes on active and inactive human X chromosomes.

10. Altered turnover of hypoxanthine phosphoribosyltransferase in erythroid cells of mice expressing Hprt a and Hprt b alleles.