Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Restoration of hypoxanthine phosphoribosyl transferase activity in mouse 1R cells after fusion with chick-embryo fibroblasts.

Literature DB >> 4516198

Restoration of hypoxanthine phosphoribosyl transferase activity in mouse 1R cells after fusion with chick-embryo fibroblasts.

B Bakay, C M Croce, H Koprowski, W L Nyhan.

Abstract

Fusion of the 1R mouse cell, which lacks activity of hypoxanthine phosphoribosyl transferase (EC 2.4.2.8), with chick-embryo fibroblasts yielded progeny cells that survived in hypoxanthine-aminopterin-thymidine selective medium. This property and the failure of the progeny to survive in 8-azaguanine indicated that hypoxanthine phosphoribosyl transferase activity was present. Electrophoretic analysis revealed that the enzyme was of mouse, not chick, origin. These observations are consistent with the operation of a regulator gene responsible for the absence of hypoxanthine phosphoribosyl-transferase activity in the 1R cell and its presence in the progeny.

Entities: CellLine Species

Mesh：

Substances：

Year: 1973 PMID： 4516198 PMCID： PMC433651 DOI： 10.1073/pnas.70.7.1998

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

16 in total

1. Induction of homokaryocyte, heterokaryocyte and hybrid formation by lysolecithin.

Authors: C M Croce; W Sawicki; D Kritchevsky; H Koprowski
Journal: Exp Cell Res Date: 1971-08 Impact factor: 3.905

2. Correction of a genetic defect in a mammalian cell.

Authors: A G Schwartz; P R Cook; H Harris
Journal: Nat New Biol Date: 1971-03-03

3. A rapid banding technique for human chromosomes.

Authors: M Seabright
Journal: Lancet Date: 1971-10-30 Impact factor: 79.321

4. Genetic analysis with human--mouse somatic cell hybrids.

Authors: M Nabholz; V Miggiano; W Bodmer
Journal: Nature Date: 1969-07-26 Impact factor: 49.962

5. Mapping of a human genetic regulator element by somatic cell genetic analysis.

Authors: R J Klebe; T Chen; R H Ruddle
Journal: Proc Natl Acad Sci U S A Date: 1970-08 Impact factor: 11.205

6. Detection of radioactive components in polyacrylamide gel disc electropherograms by automated mechanical fractionation.

Authors: B Bakay
Journal: Anal Biochem Date: 1971-04 Impact factor: 3.365

7. The use of drug-resistant markers to study the hybridization of mouse fibroblasts.

Authors: J W Littlefield
Journal: Exp Cell Res Date: 1966-01 Impact factor: 3.905

8. An improved technique for the separation of glucose 6-phosphate dehydrogenase isoenzymes by disc electrophoresis on polyacrylamide gel.

Authors: B Bakay; W L Nyhan
Journal: Biochem Genet Date: 1969-12 Impact factor: 1.890

9. The separation of adenine and hypoxanthine-guanine phosphoribosyl transferases isoenzymes by disc gel electrophoresis.

Authors: B Bakay; W L Nyhan
Journal: Biochem Genet Date: 1971-02 Impact factor: 1.890

10. Mitotic separation of two human X-linked genes in man--mouse somatic cell hybrids.

Authors: O J Miller; P R Cook; P Meera Khan; S Shin; M Siniscalco
Journal: Proc Natl Acad Sci U S A Date: 1971-01 Impact factor: 11.205

11 in total

1. Reexpression of HPRT activity following cell fusion with polyethylene glycol.

Authors: B Bakay; M Graf; S Carey; E Nissinen; W L Nyhan
Journal: Biochem Genet Date: 1978-04 Impact factor: 1.890

Review 2. Monochromosomal Hybrids and Chromosome Transfer: A Functional Approach for Gene Identification.

Authors: Raj P Kandpal; Arbans K Sandhu; Gurpreet Kaur; Gursurinder P Kaur; Raghbir S Athwal
Journal: Cancer Genomics Proteomics Date: 2017 Mar-Apr Impact factor: 4.069

3. Modulation of the activity of an avian gene transferred into a mammalian cell by cell fusion.

Authors: H P Klinger; S I Shin
Journal: Proc Natl Acad Sci U S A Date: 1974-04 Impact factor: 11.205

Review 4. Hypoxanthine-guanine phosphoribosyl transferase deficiency.

Authors: C H de Bruyn
Journal: Hum Genet Date: 1976-02-29 Impact factor: 4.132

5. Reexpression of the rat hypoxanthine phosphoribosyltransferase gene in rat-human hybrids.

Authors: C M Croce; B Bakay; W L Nyhan; H Koprowski
Journal: Proc Natl Acad Sci U S A Date: 1973-09 Impact factor: 11.205

6. Observations indicating the nature of the mutation in phenylketonuria.

Authors: K H Choo; R G Cotton; I G Jennings; D M Danks
Journal: J Inherit Metab Dis Date: 1980 Impact factor: 4.982

7. Adenine phosphoribosyltransferase and hypoxanthine-guanine phosphoribosyltransferase immunoprecipitation reactions in human-mouse and human-hamster cell hybrids.

Authors: K R Held; B Kahan; R DeMars
Journal: Humangenetik Date: 1975-10-20

8. Hypoxanthine phosphoribosyltransferase deficiency: association of reduced catalytic activity with reduced levels of immunologically detectable enzyme protein.

Authors: K S Upchurch; A Leyva; W J Arnold; E W Holmes; W N Kelley
Journal: Proc Natl Acad Sci U S A Date: 1975-10 Impact factor: 11.205

9. Discontinuous variability, in the form of a geometric progression, of albumin production in hepatoma and hybrid cells.

Authors: J A Peterson
Journal: Proc Natl Acad Sci U S A Date: 1974-05 Impact factor: 11.205

10. Analysis of HeLa cell hypoxanthine phosphoribosyltransferase mutants and revertants by two-dimensional polyacrylamide gel electrophoresis: evidence for silent gene activation.

Authors: G Milman; E Lee; G S Ghangas; J R McLaughlin; M George
Journal: Proc Natl Acad Sci U S A Date: 1976-12 Impact factor: 11.205