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Literature DB >> 28387649

Monochromosomal Hybrids and Chromosome Transfer: A Functional Approach for Gene Identification.

Raj P Kandpal¹, Arbans K Sandhu², Gurpreet Kaur², Gursurinder P Kaur², Raghbir S Athwal³.

Abstract

Functional complementation of cellular defects has been a valuable approach for localizing causative genes to specific chromosomes. The complementation strategy was followed by positional cloning and characterization of genes for their biological relevance. We herein describe strategies used for the construction of monochromosomal hybrids and their applications for cloning and characterization of genes related to cell growth, cell senescence and DNA repair. We have cloned RNaseT2, GluR6 (glutamate ionotropic receptor kainate type subunit 2-GRIK2) and protein tyrosine phosphatase, receptor type K (PTPRK) genes using these strategies. Copyright

Entities: Gene

Keywords: Chromosome transfer; GRIK-2; GluR6; cell senescence; functional cloning; glutamate ionotropic receptor kainate type subunit 2; monochromosomal hybrids; protein tyrosine phosphatase; receptor type K (PTPRK); review; senescence associated β-galactosidase

Mesh：

Substances：

Year: 2017 PMID： 28387649 PMCID： PMC5369314 DOI： 10.21873/cgp.20022

Source DB: PubMed Journal: Cancer Genomics Proteomics ISSN： 1109-6535 Impact factor: 4.069

76 in total

1. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.

Authors: H Shizuya; B Birren; U J Kim; V Mancino; T Slepak; Y Tachiiri; M Simon
Journal: Proc Natl Acad Sci U S A Date: 1992-09-15 Impact factor: 11.205

Review 2. Immunity, inflammation, and cancer.

Authors: Sergei I Grivennikov; Florian R Greten; Michael Karin
Journal: Cell Date: 2010-03-19 Impact factor: 41.582

3. The clone panel: a systematic approach to gene mapping using interspecific somatic cell hybrids.

Authors: R P Creagan; F H Ruddle
Journal: Cytogenet Cell Genet Date: 1975

4. Analysis of giant polynuclear cell formation caused by HVJ virus from Ehrlich's ascites tumor cells. I. Microscopic observation of giant polynuclear cell formation.

Authors: Y OKADA
Journal: Exp Cell Res Date: 1962-02 Impact factor: 3.905

5. Restoration of senescence in breast and ovarian cancer cells following the transfer of the YAC carrying SEN6A gene located at 6q16.3.

Authors: Neena S Rane; Arbansjit K Sandhu; Vikramjit S Zhawar; Gurpreet Kaur; Nicholas C Popescu; Raj P Kandpal; Meena Jhanwar-Uniyal; Raghbir S Athwal
Journal: Cancer Genomics Proteomics Date: 2011 Sep-Oct Impact factor: 4.069

Review 10. Four faces of cellular senescence.

Authors: Francis Rodier; Judith Campisi
Journal: J Cell Biol Date: 2011-02-14 Impact factor: 10.539

1 in total

1. Senescence of Normal Human Fibroblasts Relates to the Expression of Ionotropic Glutamate Receptor GluR6/Grik2.

Authors: Vikramjit K Zhawar; Raj P Kandpal; Raghbir S Athwal
Journal: Cancer Genomics Proteomics Date: 2020 Nov-Dec Impact factor: 4.069

1 in total

Monochromosomal Hybrids and Chromosome Transfer: A Functional Approach for Gene Identification.

1. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.

Review 2. Immunity, inflammation, and cancer.

3. The clone panel: a systematic approach to gene mapping using interspecific somatic cell hybrids.

4. Analysis of giant polynuclear cell formation caused by HVJ virus from Ehrlich's ascites tumor cells. I. Microscopic observation of giant polynuclear cell formation.

5. Restoration of senescence in breast and ovarian cancer cells following the transfer of the YAC carrying SEN6A gene located at 6q16.3.

6. A monochromosomal hybrid cell assay for evaluating the genotoxicity of environmental chemicals.

7. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.

8. Microcell-mediated transfer of murine chromosomes into mouse, Chinese hamster, and human somatic cells.

9. Construction and characterization of new coliphage M13 cloning vectors.

Review 10. Four faces of cellular senescence.

1. Senescence of Normal Human Fibroblasts Relates to the Expression of Ionotropic Glutamate Receptor GluR6/Grik2.