Literature DB >> 7192533

Enzymatic method for rapid and sensitive determination of beta-lactam antibiotics.

J M Frère, D Klein, J M Ghuysen.   

Abstract

A rapid and sensitive procedure for the estimation of beta-lactam antibiotics is described which makes use of the ability of these antibiotics to inactivate the R39 DD-carboxypeptidase. Depending on the values of the kinetic parameters which govern the reaction, the antibiotics fall into two groups. The lower limit for the quantitative estimation of the antibiotics of groups I and II is about 5 and 50 pmol/ml, respectively. The procedure has been adopted to biological fluids such as human sera and cows' milk.

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Year:  1980        PMID： 7192533      PMCID： PMC284039          DOI： 10.1128/AAC.18.4.506

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  8 in total

1.  Exocellular DD-carboxypeptidases-transpeptidases from Streptomyces.

Authors:  J M Frére; M Leyh-Bouille; J M Ghuysen; M Nieto; H R Perkins
Journal:  Methods Enzymol       Date:  1976       Impact factor: 1.600

2.  Mode of interaction between beta-lactam antibiotics and the exocellular DD-carboxypeptidase--transpeptidase from Streptomyces R39.

Authors:  N Fuad; J M Frère; J M Ghuysen; C Duez; M Iwatsubo
Journal:  Biochem J       Date:  1976-06-01       Impact factor: 3.857

3.  Simplified, accurate method for antibiotic assay of clinical specimens.

Authors:  J V Bennett; J L Brodie; E J Benner; W M Kirby
Journal:  Appl Microbiol       Date:  1966-03

4.  The peptidoglycan crosslinking enzyme system in Streptomyces R61, K15 and rimosus. Immunological studies.

Authors:  M Nguyen-Distèche; J M Frère; J Dusart; M Leyh-Bouille; J M Ghuysen
Journal:  Eur J Biochem       Date:  1977-11-15

5.  Rapid determination of amoxycillin (clamoxyl) and ampicillin (penbritin) in body fluids of many by means of high-performance liquid chromatography.

Authors:  T B Vree; Y A Hekster; A M Baars; E Van der Kleijn
Journal:  J Chromatogr       Date:  1978-05-01

6.  Molecular weight, amino acid composition and physicochemical properties of the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39.

Authors:  J M Frère; R Moreno; J M Ghuysen
Journal:  Biochem J       Date:  1974-10       Impact factor: 3.857

7.  Pharmacokinetics of amoxicillin: dose dependence after intravenous, oral, and intramuscular administration.

Authors:  D A Spyker; R J Rugloski; R L Vann; W M O'Brien
Journal:  Antimicrob Agents Chemother       Date:  1977-01       Impact factor: 5.191

8.  Metabolism of penicillins to penicilloic acids and 6-aminopenicillanic acid in man and its significance in assessing penicillin absorption.

Authors:  M Cole; M D Kenig; V A Hewitt
Journal:  Antimicrob Agents Chemother       Date:  1973-04       Impact factor: 5.191
  8 in total
  3 in total

1.  Cloning and amplified expression in Streptomyces lividans of the gene encoding the extracellular beta-lactamase of Actinomadura R39.

Authors:  C Piron-Fraipont; C Duez; A Matagne; C Molitor; J Dusart; J M Frère; J M Ghuysen
Journal:  Biochem J       Date:  1989-09-15       Impact factor: 3.857

2.  Active-site-serine D-alanyl-D-alanine-cleaving-peptidase-catalysed acyl-transfer reactions. Procedures for studying the penicillin-binding proteins of bacterial plasma membranes.

Authors:  J M Ghuysen; J M Frère; M Leyh-Bouille; M Nguyen-Distèche; J Coyette
Journal:  Biochem J       Date:  1986-04-01       Impact factor: 3.857

3.  Comparative intracellular (THP-1 macrophage) and extracellular activities of beta-lactams, azithromycin, gentamicin, and fluoroquinolones against Listeria monocytogenes at clinically relevant concentrations.

Authors:  Stéphane Carryn; Françoise Van Bambeke; Marie-Paule Mingeot-Leclercq; Paul M Tulkens
Journal:  Antimicrob Agents Chemother       Date:  2002-07       Impact factor: 5.191
  3 in total

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