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Literature DB >> 42388

Dodecyl sulphate/polyacrylamide-gel electrophoresis at low pH values and low temperatures.

Abstract

A simple method is described for dodecyl sulphate/polyacrylamide-gel electrophoresis of pH- and temperature-labile biological intermediates. The method is based on a catalyst system that works at temperatures of 2--4 degrees C and pH values of 2--4 and an appropriate buffer system containing Li+ or Tris [CH2OH--C(CH2OH)2--NH3+] instead of Na+. This system does not lead to the precipitation of 1% dodecyl sulphate.

Entities: Chemical

Mesh：

Substances：

Year: 1979 PMID： 42388 PMCID： PMC1161216 DOI： 10.1042/bj1810759

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

15 in total

1. Purification and properties of high-affinity Ca2+-ATPase of human erythrocyte membranes.

Authors: H U Wolf; G Dieckvoss; R Lichtner
Journal: Acta Biol Med Ger Date: 1977

2. Studies on (Na+ + K+)-activated ATPase. XXXVIII. A 100 000 molecular weight protein as the low-energy phosphorylated intermediate of the enzyme.

Authors: F M Schuurmans Stekhoven; M P van Heeswijk; J J de Pont; S L Bonting
Journal: Biochim Biophys Acta Date: 1976-01-23

3. Isolation and characterization of a phosphorylated intermediate in the (Na+ + K+) system-dependent ATPase.

Authors: H Bader; A K Sen; R L Post
Journal: Biochim Biophys Acta Date: 1966-04-12

4. Electrophoretic "particle sieving" in polyacrylamide gels as applied to ribosomes.

Authors: S Hjertén; S Jerstedt; A Tiselius
Journal: Anal Biochem Date: 1965-05 Impact factor: 3.365

5. Gel electrophoresis: a new catalyst for acid systems.

Authors: E M Jordan; S Raymond
Journal: Anal Biochem Date: 1969-02 Impact factor: 3.365

6. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors: K Weber; M Osborn
Journal: J Biol Chem Date: 1969-08-25 Impact factor: 5.157

7. Disk electrophoresis of wheat flour proteins with a modified apparatus utilizing gels of rectangular cross section.

Authors: K A Narayan; M Vogel; J M Lawrence
Journal: Anal Biochem Date: 1965-09 Impact factor: 3.365

8. An acrylamide gel soluble in scintillation fluids: its application to electrophoresis at neutral and low pH.

Authors: G L Choules; B H Zimm
Journal: Anal Biochem Date: 1965-11 Impact factor: 3.365

9. The red cell membrane contains three different adenosine triphophatases.

Authors: L K Drickamer
Journal: J Biol Chem Date: 1975-03-10 Impact factor: 5.157

10. Demonstration of a phosphopeptide intermediate in the Mg ++ -dependent, Na + - and K + -stimulated adenosine triphosphatase reaction of the erythrocyte membrane.

Authors: J Avruch; G Fairbanks
Journal: Proc Natl Acad Sci U S A Date: 1972-05 Impact factor: 11.205

7 in total

1. Fluorescence-based analysis of aminoacyl- and peptidyl-tRNA by low-pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors: Robert N Kirchdoerfer; Joseph J-T Huang; Molly K Isola; Silvia Cavagnero
Journal: Anal Biochem Date: 2007-01-19 Impact factor: 3.365

Dodecyl sulphate/polyacrylamide-gel electrophoresis at low pH values and low temperatures.

1. Purification and properties of high-affinity Ca2+-ATPase of human erythrocyte membranes.

2. Studies on (Na+ + K+)-activated ATPase. XXXVIII. A 100 000 molecular weight protein as the low-energy phosphorylated intermediate of the enzyme.

3. Isolation and characterization of a phosphorylated intermediate in the (Na+ + K+) system-dependent ATPase.

4. Electrophoretic "particle sieving" in polyacrylamide gels as applied to ribosomes.

5. Gel electrophoresis: a new catalyst for acid systems.

6. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

7. Disk electrophoresis of wheat flour proteins with a modified apparatus utilizing gels of rectangular cross section.

8. An acrylamide gel soluble in scintillation fluids: its application to electrophoresis at neutral and low pH.

9. The red cell membrane contains three different adenosine triphophatases.

10. Demonstration of a phosphopeptide intermediate in the Mg ++ -dependent, Na + - and K + -stimulated adenosine triphosphatase reaction of the erythrocyte membrane.

1. Fluorescence-based analysis of aminoacyl- and peptidyl-tRNA by low-pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

2. On the mechanism of the reduction by thyroid hormone of beta-adrenergic relaxation rate stimulation in rat heart.

3. Modification of the Red Beet Plasma Membrane H-ATPase by Diethylpyrocarbonate.

4. Plasma membrane ATPase of red beet forms a phosphorylated intermediate.

5. Further Characterization of the Red Beet Plasma Membrane Ca-ATPase Using GTP as an Alternative Substrate.

6. A method for the preparation of low-pH dodecyl sulphate/polyacrylamide-gradient gels.

7. Native aggregation is a common feature among triosephosphate isomerases of different species.