Literature DB >> 6272713

A method for the preparation of low-pH dodecyl sulphate/polyacrylamide-gradient gels.

G D Jones, M T Wilson, V M Darley-Usmar.   

Abstract

1. A low-pH lithium dodecyl sulphate/polyacrylamide-gradient slab-gel system, suitable for electrophoresis, is described, and the migration properties of standard proteins are compared on this and conventional high-pH gels. 2. Cytochrome oxidase may be partially resolved into its component polypeptides. The order of migration of these is, however, dependent on the pH of the gel system.

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Year:  1981        PMID： 6272713      PMCID： PMC1162698          DOI： 10.1042/bj1931013

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  8 in total

1.  Labeling of cytochrome c oxidase with [35S]diazobenzenesulfonate. Orientation of this electron transfer complex in the inner mitochondrial membrane.

Authors:  B Ludwig; N W Downer; R A Capaldi
Journal:  Biochemistry       Date:  1979-04-17       Impact factor: 3.162

2.  Isolation and characterization of a phosphorylated intermediate in the (Na+ + K+) system-dependent ATPase.

Authors:  H Bader; A K Sen; R L Post
Journal:  Biochim Biophys Acta       Date:  1966-04-12

3.  Gel chromatography of proteins in denaturing solvents. Comparison between sodium dodecyl sulfate and guanidine hydrochloride as denaturants.

Authors:  W W Fish; J A Reynolds; C Tanford
Journal:  J Biol Chem       Date:  1970-10-10       Impact factor: 5.157

4.  Reliability of molecular weight determination of proteins by polyacrylamide gradient gel electrophoresis in the presence of sodium dodecyl sulfate.

Authors:  P Lambin
Journal:  Anal Biochem       Date:  1978-03       Impact factor: 3.365

5.  Structural and functional properties of cytochrome c oxidases isolated from sharks.

Authors:  M T Wilson; W Lalla-Maharajh; V Darley-Usmar; J Bonaventura; C Bonaventura; M Brunori
Journal:  J Biol Chem       Date:  1980-04-10       Impact factor: 5.157

6.  A new method for determination of molecular weights of proteins by electrophoresis across a sodium dodecyl sulfate (SDS)-polyacrylamide gradient gel.

Authors:  P Lambin; D Rochu; J M Fine
Journal:  Anal Biochem       Date:  1976-08       Impact factor: 3.365

7.  Demonstration of a phosphopeptide intermediate in the Mg ++ -dependent, Na + - and K + -stimulated adenosine triphosphatase reaction of the erythrocyte membrane.

Authors:  J Avruch; G Fairbanks
Journal:  Proc Natl Acad Sci U S A       Date:  1972-05       Impact factor: 11.205

8.  Dodecyl sulphate/polyacrylamide-gel electrophoresis at low pH values and low temperatures.

Authors:  R Lichtner; H U Wolf
Journal:  Biochem J       Date:  1979-09-01       Impact factor: 3.857
  8 in total
  2 in total

1.  Cytoplasmic poly(ADP-ribose) polymerase and poly(ADP-ribose) glycohydrolase in AEV-transformed chicken erythroblasts.

Authors:  H Thomassin; C Martins de Sa; K Scherrer; C Maniez; P Mandel
Journal:  Mol Biol Rep       Date:  1988       Impact factor: 2.316

2.  The rapid response of isolated mitochondrial particles to 0.1 nM-tri-iodothyronine correlates with the ADP-ribosylation of a single inner-membrane protein.

Authors:  D L Hardy; J Mowbray
Journal:  Biochem J       Date:  1992-05-01       Impact factor: 3.857
  2 in total

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