| Literature DB >> 4216019 |
M Thomas, J R Cameron, R W Davis.
Abstract
A bacteriophage lambda strain has been constructed and a method developed by which DNA from potentially any source can be covalently inserted through EcoRI cohesive ends into the middle of the lambda DNA. These hybrid DNAs can infect nonrestricting Escherichia coli cells and can then propagate as plaque-forming phage. A unique feature of this lambda strain is that extra DNA in the middle of its genome is required for plaque formation. A large number of such phages have been produced with E. coli DNA and Drosophila melanogaster DNA.Entities:
Mesh:
Substances:
Year: 1974 PMID: 4216019 PMCID: PMC433931 DOI: 10.1073/pnas.71.11.4579
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN: 0027-8424 Impact factor: 11.205