Literature DB >> 4530273

Bacteriophage lambda having EcoRI endonuclease sites only in the nonessential region of the genome.

A Rambach, P Tiollais.   

Abstract

A derivative of lambda b221 that has lost by mutation all EcoRI restriction sites has been isolated by alternative growth on restrictive and nonrestrictive strains. It has an efficiency of plating equal to 1 on the restrictive strain. Genetic cross of this bacteriophage with lambda plac5 imm21 gave rise to recombinants of intermediate restricting ratios. The analysis of the EcoRI endonuclease-cleaved DNA by polyacrylamide gel electrophoresis, compared with the genetic results, has permitted identification of EcoRI endonuclease cleavage sites in the recombinants. The genotypes are: lambda plac5 CI857 sRIlambda3(0)sRIlambda2(0)sRIlambda1(0) and lambda plac5 CI857 sRIlambda2(0)sRIlambda1(0). The remaining cleavage sites, respectively, sRIlac sRIlambda4 and sRIlac sRIlambda4 sRIlambda3, are all located in a region nonessential for bacteriophage multiplication. The involvement of these mutant bacteriophages as vector for foreign genes are discussed.

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Year:  1974        PMID： 4530273      PMCID： PMC434299          DOI： 10.1073/pnas.71.10.3927

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  11 in total

1.  Transduction in Escherichia Coli K-12.

Authors:  M L Morse; E M Lederberg; J Lederberg
Journal:  Genetics       Date:  1956-01       Impact factor: 4.562

2.  Construction of biologically functional bacterial plasmids in vitro.

Authors:  S N Cohen; A C Chang; H W Boyer; R B Helling
Journal:  Proc Natl Acad Sci U S A       Date:  1973-11       Impact factor: 11.205

Review 3.  Pedigrees of some mutant strains of Escherichia coli K-12.

Authors:  B J Bachmann
Journal:  Bacteriol Rev       Date:  1972-12

4.  Mapping the DNA fragments produced by cleavage by lambda DNA with endonuclease RI.

Authors:  B Allet; P G Jeppesen; K J Katagiri; H Delius
Journal:  Nature       Date:  1973-01-12       Impact factor: 49.962

5.  Deletion mutants of bacteriophage lambda. I. Isolation and initial characterization.

Authors:  J S Parkinson; R J Huskey
Journal:  J Mol Biol       Date:  1971-03-14       Impact factor: 5.469

6.  [Mutational loss of the B-specific restriction in bacteriophage fd].

Authors:  W Arber; U Kühnlein
Journal:  Pathol Microbiol (Basel)       Date:  1967

Review 7.  DNA modification and restriction.

Authors:  W Arber; S Linn
Journal:  Annu Rev Biochem       Date:  1969       Impact factor: 23.643

8.  Transposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi80.

Authors:  J R Beckwith; E R Signer
Journal:  J Mol Biol       Date:  1966-08       Impact factor: 5.469

9.  Host-controlled modification and restriction of bacteriophage T7 by escherichia coli B.

Authors:  B Eskin; J A Lautenberger; S Linn
Journal:  J Virol       Date:  1973-06       Impact factor: 5.103

10.  Genome construction between bacterial species in vitro: replication and expression of Staphylococcus plasmid genes in Escherichia coli.

Authors:  A C Chang; S N Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  1974-04       Impact factor: 11.205
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  32 in total

1.  Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control. II. DNA fragment insertion at the vicinity of the lac UV5 promoter.

Authors:  P Charnay; A Louise; A Fritsch; D Perrin; P Tiollais
Journal:  Mol Gen Genet       Date:  1979-02-26

2.  Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control: I. DNA fragment insertion at the lacZ EcoRI restriction site.

Authors:  C Pourcel; C Marchal; A Louise; A Fritsch; P Tiollais
Journal:  Mol Gen Genet       Date:  1979-02-26

3.  Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

Authors:  A C Chang; S N Cohen
Journal:  J Bacteriol       Date:  1978-06       Impact factor: 3.490

4.  Restriction and modification in B. subtilis. Purification and general properties of a restriction endonuclease from strain R.

Authors:  S Bron; K Murray; T A Trautner
Journal:  Mol Gen Genet       Date:  1975-12-30

5.  Tests of the double-strand-break repair model for red-mediated recombination of phage lambda and plasmid lambda dv.

Authors:  D S Thaler; M M Stahl; F W Stahl
Journal:  Genetics       Date:  1987-08       Impact factor: 4.562

6.  Construction of a hybrid bacteriophage-plasmid recombinant DNA vector.

Authors:  D J Donoghue; P A Sharp
Journal:  J Bacteriol       Date:  1978-12       Impact factor: 3.490

7.  Construction and characterization of the hybrid bacteriophage lambda Charon vectors for DNA cloning.

Authors:  B G Williams; F R Blattner
Journal:  J Virol       Date:  1979-02       Impact factor: 5.103

8.  Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

Authors:  J Messing; B Gronenborn; B Müller-Hill; P Hans Hopschneider
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

9.  A specialized transducing lambda phage carrying the Escherichia coli genes for phenylalanyl-tRNA synthetase.

Authors:  H Hennecke; M Springer; A Böck
Journal:  Mol Gen Genet       Date:  1977-04-29

10.  Transformation of Streptococcus pneumoniae with S. pneumoniae-lambda phage hybrid DNA: induction of deletions.

Authors:  J P Claverys; J C Lefevre; A M Sicard
Journal:  Proc Natl Acad Sci U S A       Date:  1980-06       Impact factor: 11.205
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