| Literature DB >> 158007 |
K Umene, K Shimada, T Tsuzuki, R Mori, Y Takagi.
Abstract
An in vitro recombinant ColE1-cos lambda deoxyribonucleic acid (DNA) molecule, pKY96, has 70% of the length of lambda phage DNA. The process of lambda phage-mediated transduction of pKY96 generated a small amount of transducing phage particles containing ColE1-cos lambda DNA molecules of 80 or 101% of the length of lambda phage DNA, in addition to those containing original pKY96 DNA molecules. The newly isolated larger plasmid DNAs were transduced 100 times more efficiently than pKY96 DNA. Their structures were compared with that of a prototype pKY96 DNA, and the mechanism of the formation of these molecules is discussed.Entities:
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Year: 1979 PMID: 158007 PMCID: PMC218017 DOI: 10.1128/jb.139.3.738-747.1979
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490