Literature DB >> 4612537

Deoxysubstitution in RNA by RNA polymerase in vitro: a new approach to nucleotide sequence determinations.

G V Paddock, H C Heindell, W Salser.   

Abstract

Deoxynucleotides have been incorporated into RNA synthesized in vitro by RNA polymerase with either double-stranded or single-stranded DNA as a template. By use of this technique to block or promote cleavage at a particular phosphodiester bond, a variety of specific cleavages may be obtained with the available ribonucleases and deoxyribonuclease I. These methods should greatly increase the ease and rapidity of nucleotide sequence determinations.

Entities:  

Mesh：

Substances：

Year:  1974        PMID： 4612537      PMCID： PMC434031          DOI： 10.1073/pnas.71.12.5017

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  22 in total

1.  STUDIES ON A RIBONUCLEASE FROM USTILAGO SPHAEROGENA. I. PURIFICATION AND PROPERTIES OF THE ENZYME.

Authors:  D G GLITZ; C A DEKKER
Journal:  Biochemistry       Date:  1964-10       Impact factor: 3.162

2.  Nucleotide sequence analysis of DNA. I. Partial sequence of the cohesive ends of bacteriophage lambda and 186 DNA.

Authors:  R Wu
Journal:  J Mol Biol       Date:  1970-08       Impact factor: 5.469

3.  Nucleotide sequences from the low molecular weight ribosomal RNA of Escherichia coli.

Authors:  G G Brownlee; F Sanger
Journal:  J Mol Biol       Date:  1967-02-14       Impact factor: 5.469

4.  Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.

Authors:  G G Brownlee; F Sanger
Journal:  Eur J Biochem       Date:  1969-12

5.  A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.

Authors:  R R Burgess
Journal:  J Biol Chem       Date:  1969-11-25       Impact factor: 5.157

Review 6.  DNases and their use in the studies of primary structure of nucleic acids.

Authors:  M Laskowski
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1967

7.  Ribonuclease U 1 . Physical and chemical characterization of the purified enzyme.

Authors:  W C Kenney; C A Dekker
Journal:  Biochemistry       Date:  1971-12-21       Impact factor: 3.162

8.  Satellite DNA's in the kangaroo rat.

Authors:  F T Hatch; J A Mazrimas
Journal:  Biochim Biophys Acta       Date:  1970-11-12

9.  The ribonuclease activity of crystallized pancreatic deoxyribonuclease.

Authors:  S B Zimmerman; D Sandeen
Journal:  Anal Biochem       Date:  1966-02       Impact factor: 3.365

10.  Deoxynucleotide substitution: a new technique for sequence analysis of RNA.

Authors:  A Van de Voorde; R Rogiers; J Van Herreweghe; H Van Heuverswyn; G Volckaert; W Fiers
Journal:  Nucleic Acids Res       Date:  1974-08       Impact factor: 16.971
View more
  4 in total

1.  Differential activity staining: its use in characterization of guanylyl-specific ribonuclease in the genus Ustilago.

Authors:  A Blank; C A Dekker
Journal:  Proc Natl Acad Sci U S A       Date:  1975-12       Impact factor: 11.205

2.  DNase induced after infection of KB cells by herpes simplex virus type 1 or type 2. II. Characterization of an associated endonuclease activity.

Authors:  P J Hoffmann; Y C Cheng
Journal:  J Virol       Date:  1979-11       Impact factor: 5.103

Review 3.  The role of metals in carcinogenesis: biochemistry and metabolism.

Authors:  K W Jennette
Journal:  Environ Health Perspect       Date:  1981-08       Impact factor: 9.031

4.  The role of sequence context, nucleotide pool balance and stress in 2'-deoxynucleotide misincorporation in viral, bacterial and mammalian RNA.

Authors:  Jin Wang; Hongping Dong; Yok Hian Chionh; Megan E McBee; Sasilada Sirirungruang; Richard P Cunningham; Pei-Yong Shi; Peter C Dedon
Journal:  Nucleic Acids Res       Date:  2016-06-30       Impact factor: 16.971
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.