Literature DB >> 4119786

Mutations affecting the structure of hypoxanthine: guanine phosphoribosyltransferase in cultured Chinese hamster cells.

A L Beaudet, D J Roufa, C T Caskey.   

Abstract

14 Clones resistant to 8-azaguanine, isolated from mutagenically treated cultured cells from Chinese hamsters, were tested for loss of enzymic and immunological activities of hypoxanthine: guanine phosphoribosyltransferase. Three of the clones had no enzymic activity but reacted strongly with antiserum prepared against the enzyme (CRM(+)), indicating the presence of a defective enzyme protein, probably caused by a mutation in its structural gene. The other 11 mutants had little or no enzymic or immunologic activity. Revertant clones with positive enzymic and crossreacting immunologic activities were derived from both classes of mutants. The antiserum has been used for rapid purification of the phosphoribosyltransferase from Chinese hamster cells by immunoadsorbant affinity chromatography.

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Year:  1973        PMID： 4119786      PMCID： PMC433248          DOI： 10.1073/pnas.70.2.320

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  15 in total

1.  Beta-galactosidase. Rates of synthesis and degradation of incomplete chains.

Authors:  S Lin; I Zabin
Journal:  J Biol Chem       Date:  1972-04-10       Impact factor: 5.157

2.  Effect of ploidy and mutagens on bromodeoxyuridine resistance in haploid and diploid frog cells.

Authors:  L Mezger-Freed
Journal:  Nat New Biol       Date:  1972-02-23

3.  Sense and nonsense in the genetic code. Three exceptional triplets can serve as both chain-terminating signals and amino acid codons.

Authors:  A Garen
Journal:  Science       Date:  1968-04-12       Impact factor: 47.728

4.  Human hypoxanthine-guanine phosphoribosyltransferase. Purification and subunit structure.

Authors:  W J Arnold; W N Kelley
Journal:  J Biol Chem       Date:  1971-12-10       Impact factor: 5.157

5.  A general method for the specific isolation of peptides containing modified residues, using insoluble antibody columns.

Authors:  M Wilchek; V Bocchini; M Becker; D Givol
Journal:  Biochemistry       Date:  1971-07-20       Impact factor: 3.162

6.  Mutation rates in cells at different ploidy levels.

Authors:  M Harris
Journal:  J Cell Physiol       Date:  1971-10       Impact factor: 6.384

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  The separation of adenine and hypoxanthine-guanine phosphoribosyl transferases isoenzymes by disc gel electrophoresis.

Authors:  B Bakay; W L Nyhan
Journal:  Biochem Genet       Date:  1971-02       Impact factor: 1.890

9.  Purification of IMP:pyrophosphate phosphoribosyltransferases, catalytically incompetent enzymes in Lesch-Nyhan disease.

Authors:  C S Rubin; J Dancis; L C Yip; R C Nowinski; M E Balis
Journal:  Proc Natl Acad Sci U S A       Date:  1971-07       Impact factor: 11.205

10.  Hypoxanthine-guanine phosphoribosyltransferase deficiency: chemical agents selective for mutant or normal cultured fibroblasts in mixed and heterozygote cultures.

Authors:  W Y Fujimoto; J H Subak-Sharpe; J E Seegmiller
Journal:  Proc Natl Acad Sci U S A       Date:  1971-07       Impact factor: 11.205
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  28 in total

1.  A mammalian cell mutant with a temperature-sensitive leucyl-transfer RNA synthetase.

Authors:  L H Thompson; J L Harkins; C P Stanners
Journal:  Proc Natl Acad Sci U S A       Date:  1973-11       Impact factor: 11.205

2.  Variants of mouse myeloma cells that produce short immunoglobulin heavy chains.

Authors:  B K Birshtein; J L Preud'homme; M D Scharff
Journal:  Proc Natl Acad Sci U S A       Date:  1974-09       Impact factor: 11.205

3.  Chromosomal variation and the origin of drug-resistant mutants in mammalian cell lines.

Authors:  M Terzi
Journal:  Proc Natl Acad Sci U S A       Date:  1974-12       Impact factor: 11.205

4.  Altered enzymes in drug-resistant variants of mammalian tissue culture cells.

Authors:  J D Sharp; N E Capecchi; M R Capecchi
Journal:  Proc Natl Acad Sci U S A       Date:  1973-11       Impact factor: 11.205

5.  Genetics of the mammalian phenylalanine hydroxylase system. II. Immunological and two-dimensional gel electrophoretic studies of phenylalanine hydroxylase in cultured normal and mutant rat hepatoma cells.

Authors:  K H Choo; R G Cotton
Journal:  Biochem Genet       Date:  1979-10       Impact factor: 1.890

6.  Intraspecies transfer via total cellular DNA of the gene for hypoxanthine phosphoribosyltransferase into cultured mouse cells.

Authors:  K Willecke; M Klomfass; R Mierau; J Döhmer
Journal:  Mol Gen Genet       Date:  1979-02-26

7.  Induction of prolactin-deficient variants of GH3 rat pituitary tumor cells by ethyl methanesulfonate: reversion by 5-azacytidine, a DNA methylation inhibitor.

Authors:  R D Ivarie; J A Morris
Journal:  Proc Natl Acad Sci U S A       Date:  1982-05       Impact factor: 11.205

8.  Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants.

Authors:  D S Konecki; J Brennand; J C Fuscoe; C T Caskey; A C Chinault
Journal:  Nucleic Acids Res       Date:  1982-11-11       Impact factor: 16.971

9.  Deletion and amplification of the HGPRT locus in Chinese hamster cells.

Authors:  J C Fuscoe; R G Fenwick; D H Ledbetter; C T Caskey
Journal:  Mol Cell Biol       Date:  1983-06       Impact factor: 4.272

10.  Expression of purine overproduction in a series of 8-azaguanine-resistant diploid human lymphoblast lines.

Authors:  J E Lever; G Nuki; J E Seegmiller
Journal:  Proc Natl Acad Sci U S A       Date:  1974-07       Impact factor: 11.205
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