Literature DB >> 4114980

African swine fever: application of immunoelectroosmophoresis for the detection of antibody.

I C Pan, C J De Boer, W R Hess.   

Abstract

Thirty-three pigs in three groups of nineteen, ten, and four pigs were infected with three different African swine fever (ASF) virus isolates, respectively. All virus isolates were attenuated to varying degrees by passaging in cell cultures, and they retained sufficiently low virulence to produce subacute and chronic infections in pigs. Sera collected at various intervals were tested for antibody activity by the immunoelectroosmophoresis, agar gel diffusion precipitin, and complement-fixation tests using a modified Kolmer technique. Results clearly indicated that the immunoelectroosmophoresis test is a rapid (30 minute) and accurate method with extreme sensitivity and superior to the complement-fixation and agar gel diffusion precipitin tests in detecting antibody against ASF virus. Possible use of this method in detecting ASF virus infection is suggested.

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Year:  1972        PMID： 4114980      PMCID： PMC1319686     

Source DB:  PubMed          Journal:  Can J Comp Med        ISSN： 0008-4050


  12 in total

1.  Immunologic studies on African swine fever virus. II. Enhancing effect of normal bovine serum on the complement-fixation reaction.

Authors:  K M COWAN
Journal:  Am J Vet Res       Date:  1963-07       Impact factor: 1.156

2.  Serologic and immunologic studies with African swine fever virus.

Authors:  W A MALMQUIST
Journal:  Am J Vet Res       Date:  1963-05       Impact factor: 1.156

3.  Immunological studies on African swine fever virus. I. Elimination of the procomplementary activity of swine serum with formalin.

Authors:  K M COWAN
Journal:  J Immunol       Date:  1961-04       Impact factor: 5.422

4.  [Description of a technic simultaneously combining electrophoresis and immunological precipitation in gel: electrosyneresis].

Authors:  A BUSSARD
Journal:  Biochim Biophys Acta       Date:  1959-07

5.  Use of agar diffusion precipitation test in the diagnosis of African swine fever.

Authors:  L Coggins; W P Heuschele
Journal:  Am J Vet Res       Date:  1966-03       Impact factor: 1.156

Review 6.  African swine fever virus.

Authors:  W R Hess
Journal:  Virol Monogr       Date:  1971

7.  Studies to determine the presence of neutralizing antibody in sera and kidneys from swine recovered from African swine fever.

Authors:  C J De Boer; W R Hess; A H Dardiri
Journal:  Arch Gesamte Virusforsch       Date:  1969

8.  The antibody response in pigs inoculated with attenuated African swine fever virus.

Authors:  S S Stone; P D DeLay; E C Sharman
Journal:  Can J Comp Med       Date:  1968-07

9.  African swine fever. II. Detection of the virus in swine tissues by means of the modified direct complement-fixation test.

Authors:  P Boulanger; G L Bannister; D P Gray; G M Ruckerbauer; N G Willis
Journal:  Can J Comp Med Vet Sci       Date:  1967-01

10.  In vitro differentiation of virulent and attenuated polioviruses by their growth characteristics on MS cells.

Authors:  Y KANDA; J L MELNICK
Journal:  J Exp Med       Date:  1959-01-01       Impact factor: 14.307
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  11 in total

1.  Development of an immunoelectroosmophoresis test for the detection and typing of antibodies to vesicular stomatitis viruses.

Authors:  C R Wilks; E W Jenney; J A House
Journal:  Can J Comp Med       Date:  1984-04

Review 2.  African Swine Fever virus. Brief review.

Authors:  R C Wardley; C de M Andrade; D N Black; F L de Castro Portugal; L Enjuanes; W R Hess; C Mebus; A Ordas; D Rutili; J Sanchez Vizcaino; J D Vigario; P J Wilkinson; J F Moura Nunes; G Thomson
Journal:  Arch Virol       Date:  1983       Impact factor: 2.574

3.  A reliable enzyme linked immunosorbent assay for African swine fever using the major structural protein as antigenic reagent.

Authors:  E Tabares; M Fernandez; E Salvador-Temprano; M E Carnero; C Sanchez-Botija
Journal:  Arch Virol       Date:  1981       Impact factor: 2.574

4.  Swinepox. Virus isolation, experimental infections and the differentiation from vaccinia virus infections.

Authors:  G F De Boer
Journal:  Arch Virol       Date:  1975       Impact factor: 2.574

5.  Investigation of African swine fever in slaughtered pigs, Plateau state, Nigeria, 2004-2006.

Authors:  Olajide Adewale Owolodun; Emmanuel Tumininu Obishakin; Pius Stephen Ekong; Bitrus Yakubu
Journal:  Trop Anim Health Prod       Date:  2010-06-27       Impact factor: 1.559

6.  Counter immunoelectroosmophoresis in the diagnosis of infectious bursal disease of poultry.

Authors:  O A Durojaiye; D F Adene; A A Owoade
Journal:  Trop Anim Health Prod       Date:  1985-11       Impact factor: 1.559

7.  Solid-phase radioimmunoassay techniques for the detection of African swine fever antigen and antibody.

Authors:  J R Crowther; R C Wardley; P J Wilkinson
Journal:  J Hyg (Lond)       Date:  1979-10

8.  A solid-phase enzyme linked immunosorbent assay for the detection of African swine fever virus antigen and antibody.

Authors:  R C Wardley; E M Abu Elzein; J R Crowther; P J Wilkinson
Journal:  J Hyg (Lond)       Date:  1979-10

9.  New method of antibody detection by indirect immunoperoxidase plaque staining for serodiagnosis of African swine fever.

Authors:  I C Pan; T S Huang; W R Hess
Journal:  J Clin Microbiol       Date:  1982-10       Impact factor: 5.948

10.  Proteins specified by African swine fever virus. II. Analysis of proteins in infected cells and antigenic properties.

Authors:  E Tabarés; J Martinez; F Ruiz Gonzalvo; C Sánchez-Botija
Journal:  Arch Virol       Date:  1980       Impact factor: 2.574
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