Literature DB >> 489964

Solid-phase radioimmunoassay techniques for the detection of African swine fever antigen and antibody.

J R Crowther, R C Wardley, P J Wilkinson.   

Abstract

A solid phase radioimmunoassay (RIA) has been successfully developed to measure both African swine fever virus (ASFV) antigen and antibody. Studies show that the assay is reproducible and will detect limiting antigen concentrations equivalent to 50--500 HAD50/ml. Both direct and indirect antibody RIA have been developed and have proved to be approximately 100 times fore sensitive than the complement fixation test at present available and 1000 times more sensitive than the immuno-electro-osmophoresis test for the detection of ASFV antibody.

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Year:  1979        PMID： 489964      PMCID： PMC2129899          DOI： 10.1017/s0022172400026140

Source DB:  PubMed          Journal:  J Hyg (Lond)        ISSN： 0022-1724


  9 in total

1.  Procomplementary activity of swine serum and its elimination with periodate treatment.

Authors:  Y AKAO; R SANO; A SUGIURA; H SHIMOJO
Journal:  Jpn J Med Sci Biol       Date:  1962-08

2.  PROPAGATION AND MODIFICATION OF AFRICAN SWINE FEVER VIRUS IN CELL CULTURES.

Authors:  W R HESS; B F COX; W P HEUSCHELE; S S STONE
Journal:  Am J Vet Res       Date:  1965-01       Impact factor: 1.156

3.  Hemadsorption and cytopathic effect produced by African Swine Fever virus in swine bone marrow and buffy coat cultures.

Authors:  W A MALMQUIST; D HAY
Journal:  Am J Vet Res       Date:  1960-01       Impact factor: 1.156

4.  Comparison of direct and indirect solid-phase microradioimmunoassays for the detection of viral antigens and antiviral antibody.

Authors:  J D Rosenthal; K Hayashi; A L Notkins
Journal:  Appl Microbiol       Date:  1973-04

5.  Fluorescent antibody studies on African swine fever virus.

Authors:  W P Heuschele; L Coggins; S S Stone
Journal:  Am J Vet Res       Date:  1966-03       Impact factor: 1.156

6.  The morphological characteristics of African swine fever virus and its resemblance to tipula iridescent virus.

Authors:  J D Almeida; A P Waterson; W Plowright
Journal:  Arch Gesamte Virusforsch       Date:  1967

7.  African swine fever: comparison of four serotests on porcine serums in Spain.

Authors:  I C Pan; R Trautman; W R Hess; C J DeBoer; J Tessler; A Ordas; C S Botija; J Ovejero; M C Sanchez
Journal:  Am J Vet Res       Date:  1974-06       Impact factor: 1.156

8.  African swine fever: application of immunoelectroosmophoresis for the detection of antibody.

Authors:  I C Pan; C J De Boer; W R Hess
Journal:  Can J Comp Med       Date:  1972-07

9.  Successful prophylaxis against rabies in mice and Rhesus monkeys: the interferon system and vaccine.

Authors:  G M Baer; J H Shaddock; S A Moore; P A Yager; S S Baron; H B Levy
Journal:  J Infect Dis       Date:  1977-08       Impact factor: 5.226
  9 in total
  4 in total

Review 1.  African Swine Fever virus. Brief review.

Authors:  R C Wardley; C de M Andrade; D N Black; F L de Castro Portugal; L Enjuanes; W R Hess; C Mebus; A Ordas; D Rutili; J Sanchez Vizcaino; J D Vigario; P J Wilkinson; J F Moura Nunes; G Thomson
Journal:  Arch Virol       Date:  1983       Impact factor: 2.574

2.  A solid-phase enzyme linked immunosorbent assay for the detection of African swine fever virus antigen and antibody.

Authors:  R C Wardley; E M Abu Elzein; J R Crowther; P J Wilkinson
Journal:  J Hyg (Lond)       Date:  1979-10

3.  Effect of African swine fever on lymphocyte mitogenesis.

Authors:  R C Wardley
Journal:  Immunology       Date:  1982-05       Impact factor: 7.397

4.  Prevalence of African swine fever virus in apparently healthy domestic pigs in Uganda.

Authors:  David Kalenzi Atuhaire; Mathias Afayoa; Sylvester Ochwo; Savannah Mwesigwa; Frank Norbert Mwiine; Julius Boniface Okuni; William Olaho-Mukani; Lonzy Ojok
Journal:  BMC Vet Res       Date:  2013-12-26       Impact factor: 2.741
  4 in total

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