Literature DB >> 385771

A solid-phase enzyme linked immunosorbent assay for the detection of African swine fever virus antigen and antibody.

R C Wardley, E M Abu Elzein, J R Crowther, P J Wilkinson.   

Abstract

A solid-phase enzyme-linked immunosorbent assay was developed to measure both African swine fever virus (ASFV) antigen and antibody. Experiments showed it to be reproducible and able to detect limiting antigen concentrations of 50--500 HAD50/ml. The assay was more sensitive than those used at present to detect ASFV antibody and it is suggested that it might be of great diagnostic use in countries where African swine fever has recently appeared.

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Year:  1979        PMID： 385771      PMCID： PMC2129894          DOI： 10.1017/s0022172400026152

Source DB:  PubMed          Journal:  J Hyg (Lond)        ISSN： 0022-1724


  7 in total

1.  Procomplementary activity of swine serum and its elimination with periodate treatment.

Authors:  Y AKAO; R SANO; A SUGIURA; H SHIMOJO
Journal:  Jpn J Med Sci Biol       Date:  1962-08

2.  Hemadsorption and cytopathic effect produced by African Swine Fever virus in swine bone marrow and buffy coat cultures.

Authors:  W A MALMQUIST; D HAY
Journal:  Am J Vet Res       Date:  1960-01       Impact factor: 1.156

3.  Enzyme-labelled immunosorbent assay techniques in foot-and-mouth disease virus research.

Authors:  E M Abu Elzein; J R Crowther
Journal:  J Hyg (Lond)       Date:  1978-06

4.  Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.

Authors:  S Avrameas
Journal:  Immunochemistry       Date:  1969-01

5.  Enzyme-immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination.

Authors:  A Voller; D E Bidwell
Journal:  Br J Exp Pathol       Date:  1976-04

6.  Solid-phase radioimmunoassay techniques for the detection of African swine fever antigen and antibody.

Authors:  J R Crowther; R C Wardley; P J Wilkinson
Journal:  J Hyg (Lond)       Date:  1979-10

7.  African swine fever: application of immunoelectroosmophoresis for the detection of antibody.

Authors:  I C Pan; C J De Boer; W R Hess
Journal:  Can J Comp Med       Date:  1972-07
  7 in total
  6 in total

1.  Antibodies to some pathogenic agents in free-living wild species in Tanzania.

Authors:  C Hamblin; E C Anderson; M Jago; T Mlengeya; K Hipji
Journal:  Epidemiol Infect       Date:  1990-12       Impact factor: 2.451

2.  A reliable enzyme linked immunosorbent assay for African swine fever using the major structural protein as antigenic reagent.

Authors:  E Tabares; M Fernandez; E Salvador-Temprano; M E Carnero; C Sanchez-Botija
Journal:  Arch Virol       Date:  1981       Impact factor: 2.574

3.  A four-year survey of African swine fever in Malawi.

Authors:  J M Haresnape; S A Lungu; F D Mamu
Journal:  J Hyg (Lond)       Date:  1985-10

4.  New method of antibody detection by indirect immunoperoxidase plaque staining for serodiagnosis of African swine fever.

Authors:  I C Pan; T S Huang; W R Hess
Journal:  J Clin Microbiol       Date:  1982-10       Impact factor: 5.948

5.  Effect of African swine fever on lymphocyte mitogenesis.

Authors:  R C Wardley
Journal:  Immunology       Date:  1982-05       Impact factor: 7.397

6.  Applications of enzyme-linked immunosorbent assay in veterinary medicine: a bibliography.

Authors:  S Charan; O P Gautam
Journal:  Vet Res Commun       Date:  1984-11       Impact factor: 2.459
  6 in total

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