Literature DB >> 4291680

African swine fever. II. Detection of the virus in swine tissues by means of the modified direct complement-fixation test.

P Boulanger, G L Bannister, D P Gray, G M Ruckerbauer, N G Willis.   

Abstract

The modified direct complement-fixation test, supplemented with unheated normal calf serum, was used to demonstrate antibodies in sera of swine immunized to African swine fever virus. These antibodies did not react in the ordinary direct non-supplemented complement-fixation test.African swine fever complement-fixing antigen in infected swine tissue is not denatured by extraction with fat solvents. Consequently, good antigens devoid of non-specific reactivity were obtained by extraction with a mixture of acetone and ether. The virus was detected in infected swine tissue harvested one day after beginning of pyrexia. The modified direct complement-fixation test demonstrated cross-reactions between the six strains of virus studied.

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Year:  1967        PMID： 4291680      PMCID： PMC1494626     

Source DB:  PubMed          Journal:  Can J Comp Med Vet Sci        ISSN： 0316-5957


  11 in total

1.  Immunologic studies on African swine fever virus. II. Enhancing effect of normal bovine serum on the complement-fixation reaction.

Authors:  K M COWAN
Journal:  Am J Vet Res       Date:  1963-07       Impact factor: 1.156

2.  A complement-fixation test for identification and differentiation of immunological types of the virus of vesicular exanthema of swine.

Authors:  R A BANKOWSKI; R WICHMANN; M KUMMER
Journal:  Am J Vet Res       Date:  1953-04       Impact factor: 1.156

3.  Application Of The Complement-Fixation Test To The Demonstration Of Rinderpest Virus In The Tissue Of Infected Cattle Using Rabbit Antiserum. I. Results With The Kabete And Pendik Strains Of Virus.

Authors:  P Boulanger
Journal:  Can J Comp Med Vet Sci       Date:  1957-11

4.  A Preliminary Note On A Method Of Inactivating A Substance Or Substances In Swine Serum That Interfere With The Detection Of Antibodies By The Complementary-Fixation Test.

Authors:  P Boulanger
Journal:  Can J Comp Med Vet Sci       Date:  1954-12

5.  Technique of a Modified Direct Complement-Fixation Test for Viral Antibodies in Heat Inactivated Cattle Serum.

Authors:  P Boulanger
Journal:  Can J Comp Med Vet Sci       Date:  1960-09

6.  Avian Complement-Fixation Tests.

Authors:  C E Rice
Journal:  Can J Comp Med Vet Sci       Date:  1947-08

7.  Comparison of the Indirect and "Implemented" Direct Complement-Fixation Test in the Diagnosis of Turkey Ornithosis.

Authors:  P Boulanger; G L Bannister
Journal:  Can J Comp Med Vet Sci       Date:  1961-01

8.  HOG CHOLERA. 3. INVESTIGATION OF THE COMPLEMENT-FIXATION TEST FOR THE DETECTION OF THE VIRUS IN SWINE TISSUE.

Authors:  P BOULANGER; M APPEL; G L BANNISTER; G M RUCKERBAUER; K MORI; D P GRAY
Journal:  Can J Comp Med Vet Sci       Date:  1965-08

9.  Toxoplasmosis II. Studies of Animal Sera Using Complement-Fixation Tests.

Authors:  A Robertson; G L Bannister; G M Ruckerbauer; P Boulanger
Journal:  Can J Comp Med Vet Sci       Date:  1963-07

10.  African swine fever. I. Antiserum production.

Authors: 
Journal:  Can J Comp Med Vet Sci       Date:  1967-01
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  9 in total

1.  [Isolation of the virus of transmissible gastroenteritis of swine from cell cultures and antigenic comparison of 2 American strains].

Authors:  G C Dulac; P Boulanger; J B Phaneuf
Journal:  Can Vet J       Date:  1975-03       Impact factor: 1.008

2.  Transmissible gastroenteritis: demonstration of the virus from field specimens by means of cell culture and pig inoculation.

Authors:  G C Dulac; G M Ruckerbauer; P Boulanger
Journal:  Can J Comp Med       Date:  1977-10

3.  African swine fever: application of immunoelectroosmophoresis for the detection of antibody.

Authors:  I C Pan; C J De Boer; W R Hess
Journal:  Can J Comp Med       Date:  1972-07

4.  African swine fever. 3. The use of the agar double-diffusion precipitation test for the detection of the virus in swine tissue.

Authors:  P Boulanger; G L Bannister; D P Gray; G M Ruckerbauer; N G Willis
Journal:  Can J Comp Med Vet Sci       Date:  1967-01

5.  African swine fever. I. Antiserum production.

Authors: 
Journal:  Can J Comp Med Vet Sci       Date:  1967-01

6.  African swine fever. IV. Demonstration of the viral antigen by means of immunofluorescence.

Authors:  P Boulanger; G L Bannister; A S Greig; D P Gray; G M Ruckerbauer; N G Willis
Journal:  Can J Comp Med Vet Sci       Date:  1967-01

7.  Enzootic pneumonia of pigs: complement-fixation tests for the detection of mycoplasma antibodies in the serum of immunized rabbits and infected swine.

Authors:  P Boulanger; C L'Ecuyer
Journal:  Can J Comp Med       Date:  1968-10

8.  Methods of preparing supplementing fractions from fresh unheated bovine sera for use in modified direct complement-fixation tests.

Authors:  C E Rice
Journal:  Can J Comp Med Vet Sci       Date:  1967-06

Review 9.  Putative Role of Arthropod Vectors in African Swine Fever Virus Transmission in Relation to Their Bio-Ecological Properties.

Authors:  Sarah I Bonnet; Emilie Bouhsira; Nick De Regge; Johanna Fite; Florence Etoré; Mutien-Marie Garigliany; Ferran Jori; Laetitia Lempereur; Marie-Frédérique Le Potier; Elsa Quillery; Claude Saegerman; Timothée Vergne; Laurence Vial
Journal:  Viruses       Date:  2020-07-20       Impact factor: 5.048
  9 in total

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