Literature DB >> 4031356

Determination of reducing sugars in the nanomole range with tetrazolium blue.

C K Jue, P N Lipke.   

Abstract

Addition of sodium potassium tartrate to basic solutions of tetrazolium blue [(2,2',5,5'-tetraphenyl-3,3'-dimethoxy 4,4'-biphenylene) ditetrazolium chloride] greatly improved their efficacy as colorimetric reagents for reducing sugar determination. This modification increased sensitivity and decreased reaction time. The modified reagent can determine as little as 1 nmol of neutral sugars as well as 2-amino and N-acetyl amino sugars.

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Year:  1985        PMID: 4031356     DOI: 10.1016/0165-022x(85)90046-6

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN: 0165-022X


  22 in total

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7.  Fractionation of cellulases from the ruminal fungus Neocallimastix frontalis EB188.

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8.  Supernatant protein and cellulase activities of the anaerobic ruminal fungus Neocallimastix frontalis EB188.

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9.  A comparison of sugar indicators enables a universal high-throughput sugar-1-phosphate nucleotidyltransferase assay.

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10.  Structural and biochemical analyses of glycoside hydrolase family 26 β-mannanase from a symbiotic protist of the termite Reticulitermes speratus.

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Journal:  J Biol Chem       Date:  2014-02-25       Impact factor: 5.157

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