Literature DB >> 1664199

Fractionation of cellulases from the ruminal fungus Neocallimastix frontalis EB188.

X L Li1, R E Calza.   

Abstract

Cellulases from the ruminal fungus Neocallimastix frontalis EB188 were separated by using hydroxylapatite column chromatography. Seven carboxymethylcellulases, six avicelases, and four beta-glucosidases accounted for the majority of the activities. The separation of enzymes was confirmed by using polyacrylamide gel electrophoresis. Electrophoretic migration, analysis of hydrolysis products, and substrate specificity measurements suggested that several different cellulases were secreted in N. frontalis EB188. The possible relationship of cellulase diversity to protein glycosylation is discussed.

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Year:  1991        PMID： 1664199      PMCID： PMC183967          DOI： 10.1128/aem.57.11.3331-3336.1991

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  14 in total

1.  Measurement of saccharifying cellulase.

Authors:  M Mandels; R Andreotti; C Roche
Journal:  Biotechnol Bioeng Symp       Date:  1976

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Supernatant protein and cellulase activities of the anaerobic ruminal fungus Neocallimastix frontalis EB188.

Authors:  E M Barichievich; R E Calza
Journal:  Appl Environ Microbiol       Date:  1990-01       Impact factor: 4.792

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
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5.  Production and regulation of cellulase by two strains of the rumen anaerobic fungus Neocallimastix frontalis.

Authors:  D O Mountfort; R A Asher
Journal:  Appl Environ Microbiol       Date:  1985-05       Impact factor: 4.792

6.  A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.

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Journal:  Anal Biochem       Date:  1980-07-01       Impact factor: 3.365

7.  Cellulases and xylanase of an anaerobic rumen fungus grown on wheat straw, wheat straw holocellulose, cellulose, and xylan.

Authors:  S E Lowe; M K Theodorou; A P Trinci
Journal:  Appl Environ Microbiol       Date:  1987-06       Impact factor: 4.792

8.  Polysaccharide-degrading enzymes formed by three species of anaerobic rumen fungi grown on a range of carbohydrate substrates.

Authors:  A G Williams; C G Orpin
Journal:  Can J Microbiol       Date:  1987-05       Impact factor: 2.419

9.  Glycoside hydrolase enzymes present in the zoospore and vegetative growth stages of the rumen fungi Neocallimastix patriciarum, Piromonas communis, and an unidentified isolate, grown on a range of carbohydrates.

Authors:  A G Williams; C G Orpin
Journal:  Can J Microbiol       Date:  1987-05       Impact factor: 2.419

10.  Determination of reducing sugars in the nanomole range with tetrazolium blue.

Authors:  C K Jue; P N Lipke
Journal:  J Biochem Biophys Methods       Date:  1985-08
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  8 in total

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Authors:  X L Li; H Chen; L G Ljungdahl
Journal:  Appl Environ Microbiol       Date:  1997-12       Impact factor: 4.792

4.  Monocentric and polycentric anaerobic fungi produce structurally related cellulases and xylanases.

Authors:  X L Li; H Chen; L G Ljungdahl
Journal:  Appl Environ Microbiol       Date:  1997-02       Impact factor: 4.792

5.  The cellulase system of the anaerobic rumen fungus Neocallimastix frontalis: studies on the properties of fractions rich in endo-(1-->4)-beta-D-glucanase activity.

Authors:  T M Wood; C A Wilson; S I McCrae
Journal:  Appl Microbiol Biotechnol       Date:  1995-12       Impact factor: 4.813

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Journal:  Int J Biol Sci       Date:  2009-09-04       Impact factor: 6.580

7.  Characterization of Heterologously Expressed Acetyl Xylan Esterase1 Isolated from the Anaerobic Rumen Fungus Neocallimastix frontalis PMA02.

Authors:  Mi Kwon; Jaeyong Song; Hong-Seog Park; Hyunjin Park; Jongsoo Chang
Journal:  Asian-Australas J Anim Sci       Date:  2016-06-11       Impact factor: 2.509

Review 8.  Nucleotide and protein researches on anaerobic fungi during four decades.

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Journal:  J Anim Sci Technol       Date:  2020-03-31
  8 in total

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