Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Effect of Ca2+ ions on plasmid transformation of Streptococcus lactis protoplasts.

Literature DB >> 3936417

Effect of Ca2+ ions on plasmid transformation of Streptococcus lactis protoplasts.

Abstract

The effects of Mg2+ and Ca2+ ions on the efficiency of the plasmid transformation of lysozyme-treated Streptococcus lactis protoplasts were compared. A 33-megadalton plasmid, pLP712, coding for lactose fermentation and a 6.5-megadalton plasmid, pGB301, coding for erythromycin and chloramphenicol resistance were used as model plasmids, and S. lactis MG1614 was the recipient. Replacing Mg2+ with Ca2+ in the transformation buffer was found to increase transformant frequency more than 10-fold with both plasmids.

Entities: Chemical Disease Species

Mesh：

Substances：
Magnesium
Lactose
Calcium

Year: 1985 PMID： 3936417 PMCID： PMC291800 DOI： 10.1128/aem.50.4.1100-1102.1985

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

7 in total

1. Transformation of Streptococcus lactis Protoplasts by Plasmid DNA.

Authors: J K Kondo; L L McKay
Journal: Appl Environ Microbiol Date: 1982-05 Impact factor: 4.792

2. Location of antibiotic resistance determinants, copy control, and replication functions on the double-selective streptococcal cloning vector pGB301.

Authors: D Behnke; M S Gilmore
Journal: Mol Gen Genet Date: 1981

3. Simple and rapid method for isolating large plasmid DNA from lactic streptococci.

Authors: D G Anderson; L L McKay
Journal: Appl Environ Microbiol Date: 1983-09 Impact factor: 4.792

4. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.

Authors: S Chang; S N Cohen
Journal: Mol Gen Genet Date: 1979-01-05

5. Improved medium for lactic streptococci and their bacteriophages.

Authors: B E Terzaghi; W E Sandine
Journal: Appl Microbiol Date: 1975-06

6. Plasmid transformation of Streptococcus lactis protoplasts: optimization and use in molecular cloning.

Authors: J K Kondo; L L McKay
Journal: Appl Environ Microbiol Date: 1984-08 Impact factor: 4.792

7. Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing.

Authors: M J Gasson
Journal: J Bacteriol Date: 1983-04 Impact factor: 3.490

7 in total

11 in total

1. Effect of Proteolytic Enzymes on Transfection and Transformation of Streptococcus lactis Protoplasts.

Authors: S A Woskow; J K Kondo
Journal: Appl Environ Microbiol Date: 1987-10 Impact factor: 4.792

2. Isolation of a replication region of a large lactococcal plasmid and use in cloning of a nisin resistance determinant.

Authors: A von Wright; S Wessels; S Tynkkynen; M Saarela
Journal: Appl Environ Microbiol Date: 1990-07 Impact factor: 4.792

3. Cloning of a Streptococcus lactis subsp. lactis Chromosomal Fragment Associated with the Ability To Grow in Milk.

Authors: A von Wright; S Tynkkynen; M Suominen
Journal: Appl Environ Microbiol Date: 1987-07 Impact factor: 4.792

4. Peptide Utilization Encoded by Lactococcus lactis subsp. lactis SSL135 Chromosomal DNA.

Authors: S Tynkkynen; A von Wright; E L Syväoja
Journal: Appl Environ Microbiol Date: 1989-10 Impact factor: 4.792

5. Construction of Streptococcus lactis subsp. lactis Strains with a Single Plasmid Associated with Mucoid Phenotype.

Authors: A von Wright; S Tynkkynen
Journal: Appl Environ Microbiol Date: 1987-06 Impact factor: 4.792

6. Characterization of insertion sequence IS946, an Iso-ISS1 element, isolated from the conjugative lactococcal plasmid pTR2030.

Authors: D A Romero; T R Klaenhammer
Journal: J Bacteriol Date: 1990-08 Impact factor: 3.490

10. Secretion of TEM beta-lactamase with signal sequences isolated from the chromosome of Lactococcus lactis subsp. lactis.

Authors: M Sibakov; T Koivula; A von Wright; I Palva
Journal: Appl Environ Microbiol Date: 1991-02 Impact factor: 4.792