Literature DB >> 16347474

Effect of Proteolytic Enzymes on Transfection and Transformation of Streptococcus lactis Protoplasts.

S A Woskow1, J K Kondo.   

Abstract

With both chymotrypsin and mutanolysin used to form protoplasts, consistent transformation frequencies of 10 to 10 transformants and transfectants per mug of DNA were achieved. The procedure was used to transform protoplasts of Streptococcus cremoris CS224 at low frequency (5 transformants per mug of DNA).

Entities:  

Year:  1987        PMID： 16347474      PMCID： PMC204149          DOI： 10.1128/aem.53.10.2583-2587.1987

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  21 in total

1.  Transformation of Heat-Treated Clostridium acetobutylicum Protoplasts with pUB110 Plasmid DNA.

Authors:  Y L Lin; H P Blaschek
Journal:  Appl Environ Microbiol       Date:  1984-10       Impact factor: 4.792

2.  High-efficiency transformation of Streptococcus lactis protoplasts by plasmid DNA.

Authors:  D Simon; A Rouault; M C Chopin
Journal:  Appl Environ Microbiol       Date:  1986-08       Impact factor: 4.792

3.  The location of nisin in the producer organism, Streptococcus lactis.

Authors:  R J White; A Hurst
Journal:  J Gen Microbiol       Date:  1968-09

4.  Location of antibiotic resistance determinants, copy control, and replication functions on the double-selective streptococcal cloning vector pGB301.

Authors:  D Behnke; M S Gilmore
Journal:  Mol Gen Genet       Date:  1981

5.  Highly efficient protoplast transformation system for Streptococcus faecalis and a new Escherichia coli-S. faecalis shuttle vector.

Authors:  R Wirth; F Y An; D B Clewell
Journal:  J Bacteriol       Date:  1986-03       Impact factor: 3.490

6.  Conjugative 40-megadalton plasmid in Streptococcus lactis subsp. diacetylactis DRC3 is associated with resistance to nisin and bacteriophage.

Authors:  L L McKay; K A Baldwin
Journal:  Appl Environ Microbiol       Date:  1984-01       Impact factor: 4.792

7.  Improved lysis of group N streptococci for isolation and rapid characterization of plasmid deoxyribonucleic acid.

Authors:  T R Klaenhammer; L L McKay; K A Baldwin
Journal:  Appl Environ Microbiol       Date:  1978-03       Impact factor: 4.792

8.  Inorganic salts resistance associated with a lactose-fermenting plasmid in Streptococcus lactis.

Authors:  J D Efstathiou; L L McKay
Journal:  J Bacteriol       Date:  1977-04       Impact factor: 3.490

9.  Mutanolysin-induced spheroplasts of Streptococcus mutants are true protoplasts.

Authors:  J L Siegel; S F Hurst; E S Liberman; S E Coleman; A S Bleiweis
Journal:  Infect Immun       Date:  1981-02       Impact factor: 3.441

10.  Cloning and expression of a Streptococcus cremoris proteinase in Bacillus subtilis and Streptococcus lactis.

Authors:  J Kok; J M van Dijl; J M van der Vossen; G Venema
Journal:  Appl Environ Microbiol       Date:  1985-07       Impact factor: 4.792
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  3 in total

1.  High-Frequency Transformation, by Electroporation, of Lactococcus lactis subsp. cremoris Grown with Glycine in Osmotically Stabilized Media.

Authors:  H Holo; I F Nes
Journal:  Appl Environ Microbiol       Date:  1989-12       Impact factor: 4.792

2.  Efficient construction of Streptococcus anginosus mutants in strains of clinical origin.

Authors:  Katarzyna Obszańska; Izabella Kern-Zdanowicz; Izabela Sitkiewicz
Journal:  J Appl Genet       Date:  2018-09-26       Impact factor: 3.240

3.  Genetic construction of nisin-producing Lactococcus lactis subsp. cremoris and analysis of a rapid method for conjugation.

Authors:  J R Broadbent; J K Kondo
Journal:  Appl Environ Microbiol       Date:  1991-02       Impact factor: 4.792
  3 in total

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