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Literature DB >> 6416164

Simple and rapid method for isolating large plasmid DNA from lactic streptococci.

Abstract

A procedure for the rapid isolation of plasmid DNA larger than 30 megadaltons from lactic streptococci is described. This protocol can be used on a preparative scale to isolate sufficient quantities of plasmid DNA required for restriction analysis, cloning, or transformation experiments. A scaled-down protocol is very useful for rapidly screening the plasmid content of streptococcal strains. With this methodology, previously undetected large plasmids were observed.

Mesh：

Substances：
DNA, Bacterial

Year: 1983 PMID： 6416164 PMCID： PMC239314 DOI： 10.1128/aem.46.3.549-552.1983

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

11 in total

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Authors: T C Currier; E W Nester
Journal: Anal Biochem Date: 1976-12 Impact factor: 3.365

2. Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5.

Authors: J B Hansen; R H Olsen
Journal: J Bacteriol Date: 1978-07 Impact factor: 3.490

3. High-frequency conjugation associated with Streptococcus lactis donor cell aggregation.

Authors: M J Gasson; F L Davies
Journal: J Bacteriol Date: 1980-09 Impact factor: 3.490

4. Improved medium for lactic streptococci and their bacteriophages.

Authors: B E Terzaghi; W E Sandine
Journal: Appl Microbiol Date: 1975-06

5. Transductional evidence for plasmid linkage of lactose metabolism in streptococcus lactis C2.

Authors: L L McKay; K A Baldwin; J D Efstathiou
Journal: Appl Environ Microbiol Date: 1976-07 Impact factor: 4.792

6. Plasmid distribution and evidence for a proteinase plasmid in Streptococcus lactis C2-1.

Authors: L L McKay; K A Baldwin
Journal: Appl Microbiol Date: 1975-04

7. Plasmids, loss of lactose metabolism, and appearance of partial and full lactose-fermenting revertants in Streptococcus cremoris B1.

Authors: D G Anderson; L L McKay
Journal: J Bacteriol Date: 1977-01 Impact factor: 3.490

8. Improved lysis of group N streptococci for isolation and rapid characterization of plasmid deoxyribonucleic acid.

Authors: T R Klaenhammer; L L McKay; K A Baldwin
Journal: Appl Environ Microbiol Date: 1978-03 Impact factor: 4.792

9. Extrachromosomal elements in group N streptococci.

Authors: B R Cords; L L McKay; P Guerry
Journal: J Bacteriol Date: 1974-03 Impact factor: 3.490

10. Plasmid linkage of a bacteriocin-like substance in Streptococcus lactis subsp. diacetylactis strain WM4: transferability to Streptococcus lactis.

Authors: K M Scherwitz; K A Baldwin; L L McKay
Journal: Appl Environ Microbiol Date: 1983-05 Impact factor: 4.792

328 in total

1. Naturally occurring lactococcal plasmid pAH90 links bacteriophage resistance and mobility functions to a food-grade selectable marker.

Authors: D O' Sullivan ; R P Ross; D P Twomey; G F Fitzgerald; C Hill; A Coffey
Journal: Appl Environ Microbiol Date: 2001-02 Impact factor: 4.792

2. Genotypic analysis of Escherichia coli strains from poultry carcasses and their susceptibilities to antimicrobial agents.

Authors: I Geornaras; J W Hastings; A von Holy
Journal: Appl Environ Microbiol Date: 2001-04 Impact factor: 4.792

3. Cloning, sequencing, and expression of the pyruvate carboxylase gene in Lactococcus lactis subsp. lactis C2.

Authors: H Wang; D J O'Sullivan; K A Baldwin; L L McKay
Journal: Appl Environ Microbiol Date: 2000-03 Impact factor: 4.792

4. Gene cloning, sequencing, and inactivation of the branched-chain aminotransferase of Lactococcus lactis LM0230.

Authors: M W Atiles; E G Dudley; J L Steele
Journal: Appl Environ Microbiol Date: 2000-06 Impact factor: 4.792

5. Survival, physiology, and lysis of Lactococcus lactis in the digestive tract.

Authors: S Drouault; G Corthier; S D Ehrlich; P Renault
Journal: Appl Environ Microbiol Date: 1999-11 Impact factor: 4.792

6. Identification and characterization of an active plasmid partition mechanism for the novel Lactococcus lactis plasmid pCI2000.

Authors: K Kearney; G F Fitzgerald; J F Seegers
Journal: J Bacteriol Date: 2000-01 Impact factor: 3.490

7. Cell-associated pheromone peptide (cCF10) production and pheromone inhibition in Enterococcus faecalis.

Authors: B A Buttaro; M H Antiporta; G M Dunny
Journal: J Bacteriol Date: 2000-09 Impact factor: 3.490

8. Contribution of Lactococcus lactis cell envelope proteinase specificity to peptide accumulation and bitterness in reduced-fat Cheddar cheese.

Authors: Jeffery R Broadbent; Mary Barnes; Charlotte Brennand; Marie Strickland; Kristen Houck; Mark E Johnson; James L Steele
Journal: Appl Environ Microbiol Date: 2002-04 Impact factor: 4.792

9. Survival response and rearrangement of plasmid DNA of Lactococcus lactis during long-term starvation.

Authors: W S Kim; J H Park; J Ren; P Su; N W Dunn
Journal: Appl Environ Microbiol Date: 2001-10 Impact factor: 4.792

10. Exploitation of plasmid pMRC01 to direct transfer of mobilizable plasmids into commercial lactococcal starter strains.

Authors: R M Hickey; D P Twomey; R P Ross; C Hill
Journal: Appl Environ Microbiol Date: 2001-06 Impact factor: 4.792