Literature DB >> 107388

High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.

S Chang, S N Cohen.   

Abstract

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) transformants per microgram of supercoiled DNA. Plasmids constructed by in vitro ligation or endonuclease-generated fragments of linear plasmid DNA can also transform PEG-treated protoplasts, but at a lower frequency.

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Year:  1979        PMID： 107388     DOI： 10.1007/bf00267940

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  21 in total

1.  Construction of biologically functional bacterial plasmids in vitro.

Authors:  S N Cohen; A C Chang; H W Boyer; R B Helling
Journal:  Proc Natl Acad Sci U S A       Date:  1973-11       Impact factor: 11.205

2.  Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors:  P A Sharp; B Sugden; J Sambrook
Journal:  Biochemistry       Date:  1973-07-31       Impact factor: 3.162

3.  DNA cloning in Bacillus subtilis.

Authors:  S D Ehrlich
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

4.  Transformation of Bacillus subtilis: transforming ability of deoxyribonucleic acid in lysates of L-forms or protoplasts.

Authors:  G E Bettinger; F E Young
Journal:  J Bacteriol       Date:  1975-06       Impact factor: 3.490

5.  Transformation of plasmid DNA into Streptomyces at high frequency.

Authors:  M J Bibb; J M Ward; D A Hopwood
Journal:  Nature       Date:  1978-07-27       Impact factor: 49.962

Review 6.  Mechanism of bacterial transformation and transfection.

Authors:  N K Notani; J K Setlow
Journal:  Prog Nucleic Acid Res Mol Biol       Date:  1974

7.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

8.  Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis.

Authors:  S D Ehrlich
Journal:  Proc Natl Acad Sci U S A       Date:  1977-04       Impact factor: 11.205

9.  Bacteriocin and antibiotic resistance plasmids in Bacillus cereus and Bacillus subtilis.

Authors:  K Bernhard; H Schrempf; W Goebel
Journal:  J Bacteriol       Date:  1978-02       Impact factor: 3.490

10.  Gelatin-induced reversion of protoplasts of Bacillus subtilis to the bacillary form: electron-microscopic and physical study.

Authors:  O E Landman; A Ryter; C Fréhel
Journal:  J Bacteriol       Date:  1968-12       Impact factor: 3.490
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  355 in total

1.  Contribution of the cell wall component teichuronopeptide to pH homeostasis and alkaliphily in the alkaliphile Bacillus lentus C-125.

Authors:  R Aono; M Ito; T Machida
Journal:  J Bacteriol       Date:  1999-11       Impact factor: 3.490

2.  Toxoids of streptococcal pyrogenic exotoxin A are protective in rabbit models of streptococcal toxic shock syndrome.

Authors:  M Roggiani; J A Stoehr; S B Olmsted; Y V Matsuka; S Pillai; D H Ohlendorf; P M Schlievert
Journal:  Infect Immun       Date:  2000-09       Impact factor: 3.441

3.  Different specific activities of the monomeric and oligomeric forms of plasmid DNA in transformation of B. subtilis and E. coli.

Authors:  M Mottes; G Grandi; V Sgaramella; U Canosi; G Morelli; T A Trautner
Journal:  Mol Gen Genet       Date:  1979-07-24

4.  Efficient transformation of Bacillus thuringiensis and B. cereus via electroporation: transformation of acrystalliferous strains with a cloned delta-endotoxin gene.

Authors:  W Schurter; M Geiser; D Mathé
Journal:  Mol Gen Genet       Date:  1989-07

5.  Mobility of gentamicin resistance genes from staphylococci isolated in the United States: identification of Tn4031, a gentamicin resistance transposon from Staphylococcus epidermidis.

Authors:  W D Thomas; G L Archer
Journal:  Antimicrob Agents Chemother       Date:  1989-08       Impact factor: 5.191

6.  A method for allelic replacement that uses the conjugative transposon Tn916: deletion of the emm6.1 allele in Streptococcus pyogenes JRS4.

Authors:  M Norgren; M G Caparon; J R Scott
Journal:  Infect Immun       Date:  1989-12       Impact factor: 3.441

7.  Molecular cloning of staphylococcal enterotoxin B gene in Escherichia coli and Staphylococcus aureus.

Authors:  D M Ranelli; C L Jones; M B Johns; G J Mussey; S A Khan
Journal:  Proc Natl Acad Sci U S A       Date:  1985-09       Impact factor: 11.205

8.  Expression and inducibility in Staphylococcus aureus of the mecA gene, which encodes a methicillin-resistant S. aureus-specific penicillin-binding protein.

Authors:  K Ubukata; R Nonoguchi; M Matsuhashi; M Konno
Journal:  J Bacteriol       Date:  1989-05       Impact factor: 3.490

9.  Genetic mapping of a mutation causing an alteration in Bacillus subtilis ribosomal protein S4.

Authors:  T M Henkin; G H Chambliss
Journal:  Mol Gen Genet       Date:  1984

10.  Bacteriophage phi 29 proteins required for in vitro DNA-gp3 packaging.

Authors:  M A Bjornsti; B E Reilly; D L Anderson
Journal:  J Virol       Date:  1984-06       Impact factor: 5.103
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