Literature DB >> 3883995

The purification of shikimate dehydrogenase from Escherichia coli.

S Chaudhuri, J R Coggins.   

Abstract

A procedure was developed for the purification of shikimate dehydrogenase from Escherichia coli. Homogeneous enzyme with specific activity 1100 units/mg of protein was obtained in 21% overall yield. The subunit Mr estimated by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate was 32 000. The native Mr, estimated by gel-permeation chromatography on a TSK G2000SW column, was also 32 000. E. coli shikimate dehydrogenase is therefore a monomeric NADP-linked dehydrogenase.

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Year:  1985        PMID： 3883995      PMCID： PMC1144695          DOI： 10.1042/bj2260217

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  25 in total

1.  Gene fusion during the evolution of the tryptophan operon in enterobacteriaceae.

Authors:  G F Miozzari; C Yanofsky
Journal:  Nature       Date:  1979-02-08       Impact factor: 49.962

2.  Distribution and function of genes concerned with aromatic biosynthesis in Escherichia coli.

Authors:  J Pittard; B J Wallace
Journal:  J Bacteriol       Date:  1966-04       Impact factor: 3.490

3.  Experimentally improved reliability of ultrasensitive silver staining of protein in polyacrylamide gels.

Authors:  M Eschenbruch; R R Bürk
Journal:  Anal Biochem       Date:  1982-09-01       Impact factor: 3.365

4.  Isolation of a bifunctional domain from the pentafunctional arom enzyme complex of Neurospora crassa.

Authors:  D D Smith; J R Coggins
Journal:  Biochem J       Date:  1983-08-01       Impact factor: 3.857

5.  Purification of 5-enolpyruvylshikimate 3-phosphate synthase from Escherichia coli.

Authors:  A Lewendon; J R Coggins
Journal:  Biochem J       Date:  1983-07-01       Impact factor: 3.857

6.  Crystal structure of avian dihydrofolate reductase containing phenyltriazine and NADPH.

Authors:  K W Volz; D A Matthews; R A Alden; S T Freer; C Hansch; B T Kaufman; J Kraut
Journal:  J Biol Chem       Date:  1982-03-10       Impact factor: 5.157

7.  Aromatic amino acid biosynthesis: regulation of shikimate kinase in Escherichia coli K-12.

Authors:  B Ely; J Pittard
Journal:  J Bacteriol       Date:  1979-06       Impact factor: 3.490

8.  Yeast gene TRP5: structure, function, regulation.

Authors:  H Zalkin; C Yanofsky
Journal:  J Biol Chem       Date:  1982-02-10       Impact factor: 5.157

9.  Organization of enzymes in the polyaromatic synthetic pathway: separability in bacteria.

Authors:  M B Berlyn; N H Giles
Journal:  J Bacteriol       Date:  1969-07       Impact factor: 3.490

10.  Characterization of Neurospora crassa catabolic dehydroquinase purified from N. crassa and Escherichia coli.

Authors:  A R Hawkins; W R Reinert; N H Giles
Journal:  Biochem J       Date:  1982-06-01       Impact factor: 3.857
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  14 in total

1.  The pentafunctional arom enzyme of Saccharomyces cerevisiae is a mosaic of monofunctional domains.

Authors:  K Duncan; R M Edwards; J R Coggins
Journal:  Biochem J       Date:  1987-09-01       Impact factor: 3.857

2.  Cloning, expression, purification and preliminary crystallographic characterization of a shikimate dehydrogenase from Corynebacterium glutamicum.

Authors:  Jan Schoepe; Karsten Niefind; Shivani Chatterjee; Dietmar Schomburg
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-06-10

3.  The cloning and expression of the aroL gene from Escherichia coli K12. Purification and complete amino acid sequence of shikimate kinase II, the aroL-gene product.

Authors:  G Millar; A Lewendon; M G Hunter; J R Coggins
Journal:  Biochem J       Date:  1986-07-15       Impact factor: 3.857

4.  Purification and characterization of 3-dehydroquinase from Escherichia coli.

Authors:  S Chaudhuri; J M Lambert; L A McColl; J R Coggins
Journal:  Biochem J       Date:  1986-11-01       Impact factor: 3.857

5.  The overexpression and complete amino acid sequence of Escherichia coli 3-dehydroquinase.

Authors:  K Duncan; S Chaudhuri; M S Campbell; J R Coggins
Journal:  Biochem J       Date:  1986-09-01       Impact factor: 3.857

6.  Overexpression, crystallization and preliminary X-ray crystallographic analysis of shikimate dehydrogenase from Archaeoglobus fulgidus.

Authors:  Hyung Ho Lee
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-11-25

7.  Pigment and virulence deficiencies associated with mutations in the aroE gene of Xanthomonas oryzae pv. oryzae.

Authors:  A K Goel; L Rajagopal; R V Sonti
Journal:  Appl Environ Microbiol       Date:  2001-01       Impact factor: 4.792

8.  Overexpression, crystallization, and preliminary X-ray crystallographic analysis of shikimate dehydrogenase from Thermotoga maritima.

Authors:  Hyung Ho Lee
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-06-23

9.  Modular engineering of L-tyrosine production in Escherichia coli.

Authors:  Darmawi Juminaga; Edward E K Baidoo; Alyssa M Redding-Johanson; Tanveer S Batth; Helcio Burd; Aindrila Mukhopadhyay; Christopher J Petzold; Jay D Keasling
Journal:  Appl Environ Microbiol       Date:  2011-10-21       Impact factor: 4.792

10.  Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase.

Authors:  I A Anton; J R Coggins
Journal:  Biochem J       Date:  1988-01-15       Impact factor: 3.857
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