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Literature DB >> 2950851

Purification and characterization of 3-dehydroquinase from Escherichia coli.

S Chaudhuri, J M Lambert, L A McColl, J R Coggins.

Abstract

A procedure has been developed for the purification of 3-dehydroquinase from Escherichia coli. Homogeneous enzyme with specific activity 163 units/mg of protein was obtained in 19% overall yield. The subunit Mr estimated from polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate was 29,000. The native Mr, estimated by gel permeation chromatography on Sephacryl S-200 (superfine) and on TSK G3000SW, was in the range 52,000-58,000, indicating that the enzyme is dimeric. The catalytic properties of the enzyme have been determined and shown to be very similar to those of the biosynthetic 3-dehydroquinase component of the arom multifunctional enzyme of Neurospora crassa.

Entities: Chemical Gene Species

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Year: 1986 PMID： 2950851 PMCID： PMC1147342 DOI： 10.1042/bj2390699

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

24 in total

1. The cloning and expression of the aroL gene from Escherichia coli K12. Purification and complete amino acid sequence of shikimate kinase II, the aroL-gene product.

Authors: G Millar; A Lewendon; M G Hunter; J R Coggins
Journal: Biochem J Date: 1986-07-15 Impact factor: 3.857

2. Functional domains involved in aromatic amino acid biosynthesis.

Authors: J R Coggins; M R Boocock; M S Campbell; S Chaudhuri; J M Lambert; A Lewendon; D M Mousdale; D D Smith
Journal: Biochem Soc Trans Date: 1985-04 Impact factor: 5.407

3. The 3-dehydroquinate synthase activity of the pentafunctional arom enzyme complex of Neurospora crassa is Zn2+-dependent.

Authors: J M Lambert; M R Boocock; J R Coggins
Journal: Biochem J Date: 1985-03-15 Impact factor: 3.857

4. Experimentally improved reliability of ultrasensitive silver staining of protein in polyacrylamide gels.

Authors: M Eschenbruch; R R Bürk
Journal: Anal Biochem Date: 1982-09-01 Impact factor: 3.365

5. Isolation of a bifunctional domain from the pentafunctional arom enzyme complex of Neurospora crassa.

Authors: D D Smith; J R Coggins
Journal: Biochem J Date: 1983-08-01 Impact factor: 3.857

6. Dehydroquinate synthase from Escherichia coli: purification, cloning, and construction of overproducers of the enzyme.

Authors: J W Frost; J L Bender; J T Kadonaga; J R Knowles
Journal: Biochemistry Date: 1984-09-11 Impact factor: 3.162

7. Cloning and expression in Escherichia coli K-12 of the biosynthetic dehydroquinase function of the arom cluster gene from the eucaryote, Aspergillus nidulans.

Authors: J R Kinghorn; A R Hawkins
Journal: Mol Gen Genet Date: 1982

8. Analysis of the structure and transcription of the aro3 cluster gene in Schizosaccharomyces pombe.

Authors: N Nakanishi; M Yamamoto
Journal: Mol Gen Genet Date: 1984

9. The purification of shikimate dehydrogenase from Escherichia coli.

Authors: S Chaudhuri; J R Coggins
Journal: Biochem J Date: 1985-02-15 Impact factor: 3.857

10. Isolation of the ARO1 cluster gene of Saccharomyces cerevisiae.

Authors: F W Larimer; C C Morse; A K Beck; K W Cole; F H Gaertner
Journal: Mol Cell Biol Date: 1983-09 Impact factor: 4.272

19 in total

1. The pentafunctional arom enzyme of Saccharomyces cerevisiae is a mosaic of monofunctional domains.

Authors: K Duncan; R M Edwards; J R Coggins
Journal: Biochem J Date: 1987-09-01 Impact factor: 3.857

2. A comparison of the enzymological and biophysical properties of two distinct classes of dehydroquinase enzymes.

Authors: C Kleanthous; R Deka; K Davis; S M Kelly; A Cooper; S E Harding; N C Price; A R Hawkins; J R Coggins
Journal: Biochem J Date: 1992-03-15 Impact factor: 3.857

3. The purification and characterization of 3-dehydroquinase from Streptomyces coelicolor.

Authors: P J White; J Young; I S Hunter; H G Nimmo; J R Coggins
Journal: Biochem J Date: 1990-02-01 Impact factor: 3.857

4. Evidence from kinetic isotope studies for an enolate intermediate in the mechanism of type II dehydroquinases.

Authors: J M Harris; C Gonzalez-Bello; C Kleanthous; A R Hawkins; J R Coggins; C Abell
Journal: Biochem J Date: 1996-10-15 Impact factor: 3.857

5. A conserved surface loop in type I dehydroquinate dehydratases positions an active site arginine and functions in substrate binding.

Authors: Samuel H Light; George Minasov; Ludmilla Shuvalova; Scott N Peterson; Michael Caffrey; Wayne F Anderson; Arnon Lavie
Journal: Biochemistry Date: 2011-02-21 Impact factor: 3.162

6. Efficient independent activity of a monomeric, monofunctional dehydroquinate synthase derived from the N-terminus of the pentafunctional AROM protein of Aspergillus nidulans.

Authors: J D Moore; J R Coggins; R Virden; A R Hawkins
Journal: Biochem J Date: 1994-07-01 Impact factor: 3.857

10. Characterization of the 3-dehydroquinase domain of the pentafunctional AROM protein, and the quinate dehydrogenase from Aspergillus nidulans, and the overproduction of the type II 3-dehydroquinase from neurospora crassa.

Authors: A R Hawkins; J D Moore; A M Adeokun
Journal: Biochem J Date: 1993-12-01 Impact factor: 3.857