Literature DB >> 6214255

Characterization of Neurospora crassa catabolic dehydroquinase purified from N. crassa and Escherichia coli.

A R Hawkins, W R Reinert, N H Giles.   

Abstract

1. Neurospora crassa catabolic dehydroquinase has been purified from N. crassa and Escherichia coli. 2. Protein-sequence and gel-electrophoretic data show that apparently pure, homogeneous native dehydroquinase is a mixture of intact and proteinase-cleaved enzyme monomers. 3. Protein-sequence data and steady-state kinetics show that the catabolic dehydroquinase gene of N. crassa is expressed with fidelity in E. coli.

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Year:  1982        PMID： 6214255      PMCID： PMC1158294          DOI： 10.1042/bj2030769

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  28 in total

1.  Purification and characterization of catabolic dehydroquinase, an enzyme in the inducible quinic acid catabolic pathway of Neurospora crassa.

Authors:  J A Hautala; J W Jacobson; M E Case; N H Giles
Journal:  J Biol Chem       Date:  1975-08-10       Impact factor: 5.157

2.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

3.  Proteolytic enzymes of Neurospora crassa. Purification and some properties of five intracellular proteinases.

Authors:  D Siepen; P H Yu; M R Kula
Journal:  Eur J Biochem       Date:  1975-08-01

4.  The amino acid sequence of Neurospora NADP-specific glutamate dehydrogenase. Peptides from digestion with a staphylococcal proteinase.

Authors:  J C Wootton; A J Baron; J R Fincham
Journal:  Biochem J       Date:  1975-09       Impact factor: 3.857

5.  The inducible quinate-shikimate catabolic pathway in Neurospora crassa: induction and regulation of enzyme synthesis.

Authors:  R S Chaleff
Journal:  J Gen Microbiol       Date:  1974-04

6.  The inducible quinate-shikimate catabolic pathway in Neurospora crassa: genetic organization.

Authors:  R S Chaleff
Journal:  J Gen Microbiol       Date:  1974-04

7.  Studies on the apparent instability of Neurospora tryptophan synthase. Evidence for protease.

Authors:  P H Yu; M R Kula; H Tsai
Journal:  Eur J Biochem       Date:  1973-01-03

8.  Genetic location of certain mutations conferring recombination deficiency in Escherichia coli.

Authors:  N S Willetts; A J Clark; B Low
Journal:  J Bacteriol       Date:  1969-01       Impact factor: 3.490

9.  Genetic evidence on the organization and action of the qa-1 gene product: a protein regulating the induction of three enzymes in quinate catabolism in Neurospora crassa.

Authors:  M E Case; N H Giles
Journal:  Proc Natl Acad Sci U S A       Date:  1975-02       Impact factor: 11.205

10.  The amino acid sequence of Neurospora NADP-specific glutamate dehydrogenase. Peptic and chymotryptic peptides and the complete sequence.

Authors:  A A Holder; J C Wootton; A J Baron; G K Chambers; J R Fincham
Journal:  Biochem J       Date:  1975-09       Impact factor: 3.857
View more
  7 in total

Review 1.  Gene organization and regulation in the qa (quinic acid) gene cluster of Neurospora crassa.

Authors:  N H Giles; M E Case; J Baum; R Geever; L Huiet; V Patel; B Tyler
Journal:  Microbiol Rev       Date:  1985-09

2.  Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase.

Authors:  I A Anton; J R Coggins
Journal:  Biochem J       Date:  1988-01-15       Impact factor: 3.857

3.  Characterization of the 3-dehydroquinase domain of the pentafunctional AROM protein, and the quinate dehydrogenase from Aspergillus nidulans, and the overproduction of the type II 3-dehydroquinase from neurospora crassa.

Authors:  A R Hawkins; J D Moore; A M Adeokun
Journal:  Biochem J       Date:  1993-12-01       Impact factor: 3.857

4.  The Mycobacterium tuberculosis shikimate pathway genes: evolutionary relationship between biosynthetic and catabolic 3-dehydroquinases.

Authors:  T Garbe; S Servos; A Hawkins; G Dimitriadis; D Young; G Dougan; I Charles
Journal:  Mol Gen Genet       Date:  1991-09

5.  The purification of shikimate dehydrogenase from Escherichia coli.

Authors:  S Chaudhuri; J R Coggins
Journal:  Biochem J       Date:  1985-02-15       Impact factor: 3.857

6.  Cloning and characterization of the three enzyme structural genes QUTB, QUTC and QUTE from the quinic acid utilization gene cluster in Aspergillus nidulans.

Authors:  A R Hawkins; A J Francisco Da Silva; C F Roberts
Journal:  Curr Genet       Date:  1985       Impact factor: 3.886

7.  Sequence analysis and transformation by the catabolic 3-dehydroquinase (QUTE) gene from Aspergillus nidulans.

Authors:  A J Da Silva; H Whittington; J Clements; C Roberts; A R Hawkins
Journal:  Biochem J       Date:  1986-12-01       Impact factor: 3.857
  7 in total

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