Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 The overexpression and complete amino acid sequence of Escherichia coli 3-dehydroquinase.

Literature DB >> 3541912

The overexpression and complete amino acid sequence of Escherichia coli 3-dehydroquinase.

K Duncan, S Chaudhuri, M S Campbell, J R Coggins.

Abstract

The enzyme 3-dehydroquinase was purified in milligram quantities from an overproducing strain of Escherichia coli. The amino acid sequence was deduced from the nucleotide sequence of the aroD gene and confirmed by determining the amino acid composition of the overproduced enzyme and its N-terminal amino acid sequence. The complete polypeptide chain consists of 240 amino acid residues and has a calculated subunit Mr of 26,377. Transcript mapping revealed that aroD is a typical monocistronic gene.

Entities: Gene Species

Mesh：

Substances：

Year: 1986 PMID： 3541912 PMCID： PMC1147159 DOI： 10.1042/bj2380475

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

35 in total

1. The subunit structure of the arom multienzyme complex of Neurospora crassa. Evidence from peptide 'maps' for the identity of the subunits.

Authors: J Lumsden; J R Coggins
Journal: Biochem J Date: 1978-02-01 Impact factor: 3.857

2. A cluster-gene: evidence for one gene, one polypeptide, five enzymes.

Authors: F H Gaertner; K W Cole
Journal: Biochem Biophys Res Commun Date: 1977-03-21 Impact factor: 3.575

3. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

Authors: M Dagert; S D Ehrlich
Journal: Gene Date: 1979-05 Impact factor: 3.688

4. The occurrence of two dehydroquinases in Neurospora crassa, one constitutive and one inducible.

Authors: N H Giles; C W Partridge; S I Ahmed; M E Case
Journal: Proc Natl Acad Sci U S A Date: 1967-11 Impact factor: 11.205

5. Distribution and function of genes concerned with aromatic biosynthesis in Escherichia coli.

Authors: J Pittard; B J Wallace
Journal: J Bacteriol Date: 1966-04 Impact factor: 3.490

6. Purification and characterization of 3-dehydroquinate hydrolase and shikmate oxidoreductase. Evidence for a bifunctional enzyme.

Authors: L D Polley
Journal: Biochim Biophys Acta Date: 1978-09-11

7. A new computer method for the storage and manipulation of DNA gel reading data.

Authors: R Staden
Journal: Nucleic Acids Res Date: 1980-08-25 Impact factor: 16.971

8. The complete amino acid sequence of 3-dehydroquinate synthase of Escherichia coli K12.

Authors: G Millar; J R Coggins
Journal: FEBS Lett Date: 1986-05-05 Impact factor: 4.124

9. The serC-aro A operon of Escherichia coli. A mixed function operon encoding enzymes from two different amino acid biosynthetic pathways.

Authors: K Duncan; J R Coggins
Journal: Biochem J Date: 1986-02-15 Impact factor: 3.857

8. The overexpression, purification and complete amino acid sequence of chorismate synthase from Escherichia coli K12 and its comparison with the enzyme from Neurospora crassa.

Authors: P J White; G Millar; J R Coggins
Journal: Biochem J Date: 1988-04-15 Impact factor: 3.857

9. Unusual ancestry of dehydratases associated with quinate catabolism in Acinetobacter calcoaceticus.

Authors: D A Elsemore; L N Ornston
Journal: J Bacteriol Date: 1995-10 Impact factor: 3.490

10. Characterization of the type I dehydroquinase from Salmonella typhi.

Authors: J D Moore; A R Hawkins; I G Charles; R Deka; J R Coggins; A Cooper; S M Kelly; N C Price
Journal: Biochem J Date: 1993-10-01 Impact factor: 3.857

The overexpression and complete amino acid sequence of Escherichia coli 3-dehydroquinase.

1. The subunit structure of the arom multienzyme complex of Neurospora crassa. Evidence from peptide 'maps' for the identity of the subunits.

2. A cluster-gene: evidence for one gene, one polypeptide, five enzymes.

3. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

4. The occurrence of two dehydroquinases in Neurospora crassa, one constitutive and one inducible.

5. Distribution and function of genes concerned with aromatic biosynthesis in Escherichia coli.

6. Purification and characterization of 3-dehydroquinate hydrolase and shikmate oxidoreductase. Evidence for a bifunctional enzyme.

7. A new computer method for the storage and manipulation of DNA gel reading data.

8. The complete amino acid sequence of 3-dehydroquinate synthase of Escherichia coli K12.

9. The serC-aro A operon of Escherichia coli. A mixed function operon encoding enzymes from two different amino acid biosynthetic pathways.

10. Trans-complementable copy-number mutants of plasmid ColE1.

1. The pentafunctional arom enzyme of Saccharomyces cerevisiae is a mosaic of monofunctional domains.

2. A comparison of the enzymological and biophysical properties of two distinct classes of dehydroquinase enzymes.

3. Role of the Rep helicase gene in homologous recombination in Neisseria gonorrhoeae.

4. aro mutations in Salmonella enterica cause defects in cell wall and outer membrane integrity.

5. The purification and characterization of 3-dehydroquinase from Streptomyces coelicolor.

Review 6. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Review 7. Linkage map of Escherichia coli K-12, edition 8.

8. The overexpression, purification and complete amino acid sequence of chorismate synthase from Escherichia coli K12 and its comparison with the enzyme from Neurospora crassa.

9. Unusual ancestry of dehydratases associated with quinate catabolism in Acinetobacter calcoaceticus.

10. Characterization of the type I dehydroquinase from Salmonella typhi.