Literature DB >> 3774542

Hybridization triggered cross-linking of deoxyoligonucleotides.

T R Webb, M D Matteucci.   

Abstract

This paper reports details of the synthesis of oligodeoxynucleotides containing the modified base 5-methyl-N4,N4-ethanocytosine (Ce). The 9-fluorenylmethoxycarbonyl group is used as a protecting group for the exocyclic amines of dA and dC. This group can be removed rapidly under very mild conditions. Oligomers containing the Ce base form a cross-link when hybridized to their complementary deoxyoligonucleotides. Some of the scope and limitations of these cross-link forming oligonucleotides are reported.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3774542      PMCID： PMC311787          DOI： 10.1093/nar/14.19.7661

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

Review 1.  Complementary-addressed (sequence-specific) modification of nucleic acids.

Authors:  D G Knorre; V V Vlassov
Journal:  Prog Nucleic Acid Res Mol Biol       Date:  1985

2.  Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli.

Authors:  D Pennica; W E Holmes; W J Kohr; R N Harkins; G A Vehar; C A Ward; W F Bennett; E Yelverton; P H Seeburg; H L Heyneker; D V Goeddel; D Collen
Journal:  Nature       Date:  1983-01-20       Impact factor: 49.962

3.  Sequence-specific crosslinking agents for nucleic acids. Use of 6-bromo-5,5-dimethoxyhexanohydrazide for crosslinking cytidine to guanosine and crosslinking RNA to complementary sequences of DNA.

Authors:  J Summerton; P A Bartlett
Journal:  J Mol Biol       Date:  1978-06-25       Impact factor: 5.469

4.  Modified oligonucleotides as alternatives to the synthesis of mixed probes for the screening of cDNA libraries.

Authors:  T Huynh-Dinh; N Duchange; M M Zakin; A Lemarchand; J Igolen
Journal:  Proc Natl Acad Sci U S A       Date:  1985-11       Impact factor: 11.205

5.  Specific inhibition of mRNA translation by complementary oligonucleotides covalently linked to intercalating agents.

Authors:  J J Toulmé; H M Krisch; N Loreau; N T Thuong; C Hélène
Journal:  Proc Natl Acad Sci U S A       Date:  1986-03       Impact factor: 11.205

6.  Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product.

Authors:  N D Sinha; J Biernat; J McManus; H Köster
Journal:  Nucleic Acids Res       Date:  1984-06-11       Impact factor: 16.971

7.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

8.  Analytical studies of 'mixed sequence' oligodeoxyribonucleotides synthesized by competitive coupling of either methyl- or beta-cyanoethyl-N,N-diisopropylamino phosphoramidite reagents, including 2'-deoxyinosine.

Authors:  G Zon; K A Gallo; C J Samson; K L Shao; M F Summers; R A Byrd
Journal:  Nucleic Acids Res       Date:  1985-11-25       Impact factor: 16.971
  8 in total
  18 in total

1.  Nucleic acid duplexes incorporating a dissociable covalent base pair.

Authors:  K Gao; L E Orgel
Journal:  Proc Natl Acad Sci U S A       Date:  1999-12-21       Impact factor: 11.205

2.  Trioligonucleotide reagents: addressed RNA modification.

Authors:  S I Oshevski
Journal:  Dokl Biochem Biophys       Date:  2010 Jan-Feb       Impact factor: 0.788

3.  Sequence-dependent cleavage of DNA by alkylation with antisense oligodeoxyribonucleotides containing a 2-(N-iodoacetylaminoethyl)thio-adenine.

Authors:  K Kido; H Inoue; E Ohtsuka
Journal:  Nucleic Acids Res       Date:  1992-03-25       Impact factor: 16.971

4.  Solid-supported ligation primer.

Authors:  S Pochet; B Arcangioli; T Huynh-Dinh
Journal:  Nucleic Acids Res       Date:  1988-02-25       Impact factor: 16.971

5.  Optimization of the efficiency of cross-linking PtII oligonucleotide phosphorothioate complexes to complementary oligonucleotides.

Authors:  B C Chu; L E Orgel
Journal:  Nucleic Acids Res       Date:  1990-09-11       Impact factor: 16.971

6.  Synthesis of DNA fragments containing 5,6-dihydrothymine, a major product of thymine gamma radiolysis.

Authors:  J C Schulhof; D Molko; R Teoule
Journal:  Nucleic Acids Res       Date:  1988-01-11       Impact factor: 16.971

7.  A simple method for N-15 labelling of exocyclic amino groups in synthetic oligodeoxynucleotides.

Authors:  M Acedo; C Fàbrega; A Aviño; M Goodman; P Fagan; D Wemmer; R Eritja
Journal:  Nucleic Acids Res       Date:  1994-08-11       Impact factor: 16.971

8.  Synthesis and characterization of oligodeoxynucleotides containing the mutagenic base analogue 4-O-ethylthymine.

Authors:  D Fernandez-Forner; Y Palom; S Ikuta; E Pedroso; R Eritja
Journal:  Nucleic Acids Res       Date:  1990-10-11       Impact factor: 16.971

9.  Determination of the order of substrate addition to MspI DNA methyltransferase using a novel mechanism-based inhibitor.

Authors:  C Taylor; K Ford; B A Connolly; D P Hornby
Journal:  Biochem J       Date:  1993-04-15       Impact factor: 3.857

10.  Synthesis of Oligodeoxynucleotides Containing Electrophilic Groups.

Authors:  Xi Lin; Jinsen Chen; Shahien Shahsavari; Nathanael Green; Deepti Goyal; Shiyue Fang
Journal:  Org Lett       Date:  2016-07-22       Impact factor: 6.005
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.