Literature DB >> 3858856

Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence.

C Tschudi, A S Young, L Ruben, C L Patton, F F Richards.   

Abstract

In Trypanosoma brucei gambiense, the Ca2+ binding protein calmodulin is encoded by three identical tandemly repeated genes. The transcripts of these genes consist of several RNA species similar in size. A 35-nucleotide spliced leader sequence is present at the 5' end of each mRNA but is not encoded by DNA contiguous to these genes. We have identified two different sites for the fusion of the leader to the mRNA. These results strongly support the idea that a novel, possibly discontinuous, transcription mechanism is used by these parasites.

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Year:  1985        PMID： 3858856      PMCID： PMC397921          DOI： 10.1073/pnas.82.12.3998

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  26 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Isolation of cloned genes differentially expressed at early and late stages of Drosophila embryonic development.

Authors:  G Scherer; J Telford; C Baldari; V Pirrotta
Journal:  Dev Biol       Date:  1981-09       Impact factor: 3.582

3.  RNA splicing is required to make the messenger RNA for a variant surface antigen in trypanosomes.

Authors:  L H Van der Ploeg; A Y Liu; P A Michels; T De Lange; P Borst; H K Majumder; H Weber; G H Veeneman; J Van Boom
Journal:  Nucleic Acids Res       Date:  1982-06-25       Impact factor: 16.971

4.  A catalogue of splice junction sequences.

Authors:  S M Mount
Journal:  Nucleic Acids Res       Date:  1982-01-22       Impact factor: 16.971

5.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

6.  A cloned calmodulin structural gene probe is complementary to DNA sequences from diverse species.

Authors:  R P Munjaal; T Chandra; S L Woo; J R Dedman; A R Means
Journal:  Proc Natl Acad Sci U S A       Date:  1981-04       Impact factor: 11.205

7.  Conservation of high efficiency promoter sequences in Saccharomyces cerevisiae.

Authors:  M J Dobson; M F Tuite; N A Roberts; A J Kingsman; S M Kingsman; R E Perkins; S C Conroy; L A Fothergill
Journal:  Nucleic Acids Res       Date:  1982-04-24       Impact factor: 16.971

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.

Authors:  M Carlson; D Botstein
Journal:  Cell       Date:  1982-01       Impact factor: 41.582

10.  Trypanosome mRNAs share a common 5' spliced leader sequence.

Authors:  M Parsons; R G Nelson; K P Watkins; N Agabian
Journal:  Cell       Date:  1984-08       Impact factor: 41.582
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  53 in total

1.  Stable integrative transformation of Trypanosoma brucei that occurs exclusively by homologous recombination.

Authors:  J Eid; B Sollner-Webb
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205

2.  Evolution of EF-hand calcium-modulated proteins. I. Relationships based on amino acid sequences.

Authors:  N D Moncrief; R H Kretsinger; M Goodman
Journal:  J Mol Evol       Date:  1990-06       Impact factor: 2.395

3.  Structure and expression of a tandem gene pair in Leishmania donovani that encodes a protein structurally homologous to eucaryotic cation-transporting ATPases.

Authors:  J C Meade; J Shaw; S Lemaster; G Gallagher; J R Stringer
Journal:  Mol Cell Biol       Date:  1987-11       Impact factor: 4.272

4.  Identification of a new EF-hand superfamily member from Trypanosoma brucei.

Authors:  S Wong; R H Kretsinger; D A Campbell
Journal:  Mol Gen Genet       Date:  1992-05

5.  Primary structures of Arabidopsis calmodulin isoforms deduced from the sequences of cDNA clones.

Authors:  V Ling; I Perera; R E Zielinski
Journal:  Plant Physiol       Date:  1991-08       Impact factor: 8.340

6.  A mutant Paramecium with a defective calcium-dependent potassium conductance has an altered calmodulin: a nonlethal selective alteration in calmodulin regulation.

Authors:  W H Schaefer; R D Hinrichsen; A Burgess-Cassler; C Kung; I A Blair; D M Watterson
Journal:  Proc Natl Acad Sci U S A       Date:  1987-06       Impact factor: 11.205

7.  A putative flagellar Ca2(+)-binding protein of the flagellum of trypanosomatid protozoan parasites.

Authors:  M G Lee; J F Chen; A W Ho; P A D'Alesandro; L H Van der Ploeg
Journal:  Nucleic Acids Res       Date:  1990-07-25       Impact factor: 16.971

8.  Cloning of a trypanosomatid gene coding for an ornithine decarboxylase that is metabolically unstable even though it lacks the C-terminal degradation domain.

Authors:  F Svensson; C Ceriani; E L Wallström; I Kockum; I D Algranati; O Heby; L Persson
Journal:  Proc Natl Acad Sci U S A       Date:  1997-01-21       Impact factor: 11.205

9.  Evolutionary aspects of trypanosomes: analysis of genes.

Authors:  P A Michels
Journal:  J Mol Evol       Date:  1986       Impact factor: 2.395

10.  The U6 small nuclear RNA from Trypanosoma brucei.

Authors:  C Tschudi; A R Krainer; E Ullu
Journal:  Nucleic Acids Res       Date:  1988-12-09       Impact factor: 16.971
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