Literature DB >> 3855537

Injected anti-sense RNAs specifically block messenger RNA translation in vivo.

D A Melton.   

Abstract

As a test for a method to analyze gene function in embryogenesis, the translation of a specific mRNA has been blocked in vivo by microinjection of complementary (anti-sense) RNA. RNA complementary to globin mRNA was synthesized in vitro by transcription of an inverted globin cDNA clone. After injection into frog oocyte cytoplasm, the anti-sense globin RNA forms a hybrid with globin mRNA and selectively prevents its translation. Deletion mapping of the anti-sense RNA shows that the 5' region of the globin mRNA must be covered in order to block translation. This method may allow one to study the function of many genes for which DNA clones are available by preventing the expression of the endogenous gene as protein.

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Year:  1985        PMID： 3855537      PMCID： PMC396988          DOI： 10.1073/pnas.82.1.144

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

1.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

Authors:  D A Melton; P A Krieg; M R Rebagliati; T Maniatis; K Zinn; M R Green
Journal:  Nucleic Acids Res       Date:  1984-09-25       Impact factor: 16.971

2.  Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.

Authors:  P A Krieg; D A Melton
Journal:  Nucleic Acids Res       Date:  1984-09-25       Impact factor: 16.971

3.  A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA).

Authors:  T Mizuno; M Y Chou; M Inouye
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

4.  The use of RNAs complementary to specific mRNAs to regulate the expression of individual bacterial genes.

Authors:  J Coleman; P J Green; M Inouye
Journal:  Cell       Date:  1984-06       Impact factor: 41.582

5.  Base pairing of RNA I with its complementary sequence in the primer precursor inhibits ColE1 replication.

Authors:  R M Lacatena; G Cesareni
Journal:  Nature       Date:  1981-12-17       Impact factor: 49.962

6.  Inhibition of thymidine kinase gene expression by anti-sense RNA: a molecular approach to genetic analysis.

Authors:  J G Izant; H Weintraub
Journal:  Cell       Date:  1984-04       Impact factor: 41.582

7.  Translational control of IS10 transposition.

Authors:  R W Simons; N Kleckner
Journal:  Cell       Date:  1983-09       Impact factor: 41.582

8.  Selective inhibition of Escherichia coli protein synthesis and growth by nonionic oligonucleotides complementary to the 3' end of 16S rRNA.

Authors:  K Jayaraman; K McParland; P Miller; P O Ts'o
Journal:  Proc Natl Acad Sci U S A       Date:  1981-03       Impact factor: 11.205

9.  H3 and H4 histone cDNA sequences from Xenopus: a sequence comparison of H4 genes.

Authors:  P C Turner; H R Woodland
Journal:  Nucleic Acids Res       Date:  1982-06-25       Impact factor: 16.971

Review 10.  Nuclear RNA-protein interactions and messenger RNA processing.

Authors:  T Pederson
Journal:  J Cell Biol       Date:  1983-11       Impact factor: 10.539
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  115 in total

1.  Complementarity between the mRNA 5' untranslated region and 18S ribosomal RNA can inhibit translation.

Authors:  S B Verrier; O Jean-Jean
Journal:  RNA       Date:  2000-04       Impact factor: 4.942

2.  Site specificity of the inhibitory effects of oligo(nucleoside methylphosphonate)s complementary to the acceptor splice junction of herpes simplex virus type 1 immediate early mRNA 4.

Authors:  M Kulka; C C Smith; L Aurelian; R Fishelevich; K Meade; P Miller; P O Ts'o
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

3.  Specificity of antisense oligonucleotides in vivo.

Authors:  T M Woolf; D A Melton; C G Jennings
Journal:  Proc Natl Acad Sci U S A       Date:  1992-08-15       Impact factor: 11.205

4.  Spontaneous muscle action potentials fail to develop without fetal-type acetylcholine receptors.

Authors:  Masazumi Takahashi; Tai Kubo; Akira Mizoguchi; C George Carlson; Katsuaki Endo; Katsunori Ohnishi
Journal:  EMBO Rep       Date:  2002-07       Impact factor: 8.807

Review 5.  Use of Xenopus oocytes for the functional expression of plasma membrane proteins.

Authors:  E Sigel
Journal:  J Membr Biol       Date:  1990-09       Impact factor: 1.843

6.  Inhibition of gene expression by a short sense fragment.

Authors:  F H Cameron; P A Jennings
Journal:  Nucleic Acids Res       Date:  1991-02-11       Impact factor: 16.971

7.  Organ-specific modulation of gene expression in transgenic plants using antisense RNA.

Authors:  M Cannon; J Platz; M O'Leary; C Sookdeo; F Cannon
Journal:  Plant Mol Biol       Date:  1990-07       Impact factor: 4.076

8.  Cleavage of collagen RNA transcripts by hammerhead ribozymes in vitro is mutation-specific and shows competitive binding effects.

Authors:  G Grassi; A Forlino; J C Marini
Journal:  Nucleic Acids Res       Date:  1997-09-01       Impact factor: 16.971

9.  Specific gene suppression by engineered ribozymes in monkey cells.

Authors:  F H Cameron; P A Jennings
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

10.  Antisense suppression of skeletal muscle myosin light chain-1 biosynthesis impairs myofibrillogenesis in cultured myotubes.

Authors:  R Nawrotzki; D A Fischman; T Mikawa
Journal:  J Muscle Res Cell Motil       Date:  1995-02       Impact factor: 2.698
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