Literature DB >> 6207484

Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.

P A Krieg, D A Melton.   

Abstract

We describe a method for the synthesis of microgram quantities of eucaryotic messenger RNAs. Injection into the cytoplasm of frog oocytes and addition to wheat germ extracts show that these synthetic RNAs function efficiently as messenger RNAs. We confirm that a 5' cap on the mRNA is essential for translation in injected oocytes and show that most of the 3' flanking region, including the poly A tail, can be deleted without the abolition of protein synthesis. The method of mRNA synthesis involves in vitro transcription of cDNAs which have been cloned into SP6 vectors (described in the accompanying paper). This method enables one to produce large amounts of mRNA and consequently protein from any cDNA clone.

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Year:  1984        PMID： 6207484      PMCID： PMC320142          DOI： 10.1093/nar/12.18.7057

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  31 in total

1.  Readenylation of polyadenylate-free globin messenger RNA restores its stability in vivo.

Authors:  G Huez; G Marbaix; E Hubert; Y Cleuter; M Leclercq; H Chantrenne; R Devos; H Soreq; U Nudel; U Z Littauer
Journal:  Eur J Biochem       Date:  1975-11-15

2.  Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.

Authors:  R A Laskey; A D Mills
Journal:  Eur J Biochem       Date:  1975-08-15

3.  Simian virus 40 DNA directs synthesis of authentic viral polypeptides in a linked transcription-translation cell-free system.

Authors:  B E Roberts; M Gorecki; R C Mulligan; K J Danna; S Rozenblatt; A Rich
Journal:  Proc Natl Acad Sci U S A       Date:  1975-05       Impact factor: 11.205

4.  The degradation of ribonucleic acids injected into Xenopus laevis oocytes.

Authors:  C C Allende; J E Allende; R A Firtel
Journal:  Cell       Date:  1974-07       Impact factor: 41.582

5.  Role of the polyadenylate segment in the translation of globin messenger RNA in Xenopus oocytes.

Authors:  G Huez; G Marbaix; E Hubert; M Leclercq; U Nudel; H Soreq; R Salomon; B Lebleu; M Revel; U Z Littauer
Journal:  Proc Natl Acad Sci U S A       Date:  1974-08       Impact factor: 11.205

6.  Message stability in injected frog oocytes: long life of mammalian alpha and beta globin messages.

Authors:  J B Gurdon; J B Lingrel; G Marbaix
Journal:  J Mol Biol       Date:  1973-11-05       Impact factor: 5.469

7.  Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.

Authors:  J B Gurdon; C D Lane; H R Woodland; G Marbaix
Journal:  Nature       Date:  1971-09-17       Impact factor: 49.962

8.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 9.  Processing of RNA.

Authors:  R P Perry
Journal:  Annu Rev Biochem       Date:  1976       Impact factor: 23.643

10.  Purification of mRNA guanylyltransferase and mRNA (guanine-7-) methyltransferase from vaccinia virions.

Authors:  S A Martin; E Paoletti; B Moss
Journal:  J Biol Chem       Date:  1975-12-25       Impact factor: 5.157
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  462 in total

1.  Axis determination by inhibition of Wnt signaling in Xenopus.

Authors:  K Itoh; S Y Sokol
Journal:  Genes Dev       Date:  1999-09-01       Impact factor: 11.361

2.  Kinetics of ribosomal pausing during programmed -1 translational frameshifting.

Authors:  J D Lopinski; J D Dinman; J A Bruenn
Journal:  Mol Cell Biol       Date:  2000-02       Impact factor: 4.272

3.  Membrane stretch affects gating modes of a skeletal muscle sodium channel.

Authors:  I V Tabarean; P Juranka; C E Morris
Journal:  Biophys J       Date:  1999-08       Impact factor: 4.033

4.  Inhibition of the Wnt signaling pathway by Idax, a novel Dvl-binding protein.

Authors:  S Hino; S Kishida; T Michiue; A Fukui; I Sakamoto; S Takada; M Asashima; A Kikuchi
Journal:  Mol Cell Biol       Date:  2001-01       Impact factor: 4.272

5.  Cloning and characterization of full-length mouse thymidine kinase 2: the N-terminal sequence directs import of the precursor protein into mitochondria.

Authors:  L Wang; S Eriksson
Journal:  Biochem J       Date:  2000-10-15       Impact factor: 3.857

6.  A novel karyoskeletal protein: characterization of protein NO145, the major component of nucleolar cortical skeleton in Xenopus oocytes.

Authors:  S Kneissel; W W Franke; J G Gall; H Heid; S Reidenbach; M Schnölzer; H Spring; H Zentgraf; M S Schmidt-Zachmann
Journal:  Mol Biol Cell       Date:  2001-12       Impact factor: 4.138

7.  Conservation of Pax 6 function and upstream activation by Notch signaling in eye development of frogs and flies.

Authors:  Yasuko Onuma; Shuji Takahashi; Makoto Asashima; Shoichiro Kurata; Walter J Gehring
Journal:  Proc Natl Acad Sci U S A       Date:  2002-02-12       Impact factor: 11.205

8.  Inhibitory effect of myb7 uORF on downstream gene expression in homologous (rice) and heterologous (tobacco) systems.

Authors:  Franca Locatelli; Enrico Magnani; Cristina Vighi; Chiara Lanzanova; Immacolata Coraggio
Journal:  Plant Mol Biol       Date:  2002-02-01       Impact factor: 4.076

9.  FLP and Cre recombinase function in Xenopus embryos.

Authors:  D Werdien; G Peiler; G U Ryffel
Journal:  Nucleic Acids Res       Date:  2001-06-01       Impact factor: 16.971

10.  Functional expression of the epithelial Ca(2+) channels (TRPV5 and TRPV6) requires association of the S100A10-annexin 2 complex.

Authors:  Stan F J van de Graaf; Joost G J Hoenderop; Dimitra Gkika; Dennis Lamers; Jean Prenen; Ursula Rescher; Volker Gerke; Olivier Staub; Bernd Nilius; René J M Bindels
Journal:  EMBO J       Date:  2003-04-01       Impact factor: 11.598
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