Literature DB >> 320200

Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

D W Cleveland, S G Fischer, M W Kirschner, U K Laemmli.   

Abstract

A rapid and convenient method for peptide mapping of proteins has been developed. The technique, which is especially suitable for analysis of proteins that have been isolated from gels containg sodium dodecyl sulfate, involves partial enzymatic proteolysis in the presence of sodium dodecyl sulfate and analysis of the cleavage products by polyacrylamide gel electrophoresis. The pattern of peptide fragments produced is characteristic of the protein substrate and the proteolytic enzyme and is highly reproducible. Several common proteases have been used including chymotrypsin, Staphylococcus aureus protease, and papain.

Entities:  

Mesh：

Substances：

Year:  1977        PMID： 320200

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  1554 in total

1.  Purification and characterization of the hepatic CYP2C and 3A isozymes from phenobarbitone pretreated rhesus monkey.

Authors:  K V Ramana; K K Kohli
Journal:  Mol Cell Biochem       Date:  1999-08       Impact factor: 3.396

2.  An abundant DNA binding protein from the hyperthermophilic archaeon Sulfolobus shibatae affects DNA supercoiling in a temperature-dependent fashion.

Authors:  H Xue; R Guo; Y Wen; D Liu; L Huang
Journal:  J Bacteriol       Date:  2000-07       Impact factor: 3.490

3.  Toc34 is a preprotein receptor regulated by GTP and phosphorylation.

Authors:  N Sveshnikova; J Soll; E Schleiff
Journal:  Proc Natl Acad Sci U S A       Date:  2000-04-25       Impact factor: 11.205

4.  Two overlapping reading frames in a single exon encode interacting proteins--a novel way of gene usage.

Authors:  M Klemke; R H Kehlenbach; W B Huttner
Journal:  EMBO J       Date:  2001-07-16       Impact factor: 11.598

5.  Controlling tumor-derived and vascular endothelial cell growth: role of the 4Ff2 cell surface antigen.

Authors:  M Papetti; I M Herman
Journal:  Am J Pathol       Date:  2001-07       Impact factor: 4.307

6.  Glycogen synthase kinase 3 phosphorylates kinesin light chains and negatively regulates kinesin-based motility.

Authors:  Gerardo Morfini; Györgyi Szebenyi; Ravindhra Elluru; Nancy Ratner; Scott T Brady
Journal:  EMBO J       Date:  2002-02-01       Impact factor: 11.598

7.  Bacterial senescence: protein oxidation in non-proliferating cells is dictated by the accuracy of the ribosomes.

Authors:  M Ballesteros; A Fredriksson; J Henriksson; T Nyström
Journal:  EMBO J       Date:  2001-09-17       Impact factor: 11.598

8.  Determination of essential amino acids involved in the CD4-independent tropism of the X4 human immunodeficiency virus type 1 m7NDK isolate: role of potential N glycosylations in the C2 and V3 regions of gp120.

Authors:  J Dumonceaux; C Goujon; V Joliot; P Briand; U Hazan
Journal:  J Virol       Date:  2001-06       Impact factor: 5.103

9.  Two processing steps in maturation of vitellogenin polypeptides in Drosophila melanogaster.

Authors:  T G Warren; M D Brennan; A P Mahowald
Journal:  Proc Natl Acad Sci U S A       Date:  1979-06       Impact factor: 11.205

10.  Purification of measles virus and characterization of subviral components.

Authors:  K C Stallcup; S L Wechsler; B N Fields
Journal:  J Virol       Date:  1979-04       Impact factor: 5.103
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.