Literature DB >> 381841

Expression of Escherichia coli ribosomal protein and RNA polymerase genes cloned on plasmids.

N P Fiil, D Bendiak, J Collins, J D Friesen.   

Abstract

Fragments of lambda drifd 18 DNA with different end-points within the set of structural genes of ribosomal proteins L11 (RPLK), Li (rplA), L10 (rplJ) and L12 (rplL) as well as the beta (rpoB) ANd beta' (rpoC) subunits of RNA polymerase have been cloned on plasmids. These plasmids were transformed in host cells which were mutant for each of the genes, enabling expression of both wild-type (plasmid-borne) and mutant (chromosomal) genes to be differentiated. On the basis of these results we propose the following genetic structure for the region: rplK and rplA are in one operon; rplL, rpoB and rpoC are in a second. Our data suggest the possibility that rplJ is by itself in an operon situated between the other two.

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Year:  1979        PMID： 381841     DOI： 10.1007/bf00267689

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  30 in total

1.  Patterns of protein synthesis in E. coli: a catalog of the amount of 140 individual proteins at different growth rates.

Authors:  S Pedersen; P L Bloch; S Reeh; F C Neidhardt
Journal:  Cell       Date:  1978-05       Impact factor: 41.582

2.  Evidence for a lambda transducing phage carrying the genes for the beta and beta' subunits of Escherichia coli RNA polymerase.

Authors:  J B Kirschbaum; J Scaife
Journal:  Mol Gen Genet       Date:  1974

3.  Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors:  P A Sharp; B Sugden; J Sambrook
Journal:  Biochemistry       Date:  1973-07-31       Impact factor: 3.162

4.  Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.

Authors:  D B Clewell; D R Helinski
Journal:  Biochemistry       Date:  1970-10-27       Impact factor: 3.162

5.  New chromosomal location for structural genes of ribosomal proteins.

Authors:  R J Watson; J Parker; N P Fiil; J G Flaks; J D Friesen
Journal:  Proc Natl Acad Sci U S A       Date:  1975-07       Impact factor: 11.205

6.  Syntheses of elongation factors Tu and G are under stringent control in Escherichia coli.

Authors:  A V Furano; F P Wittel
Journal:  J Biol Chem       Date:  1976-02-10       Impact factor: 5.157

7.  Isolation and characterization of temperature-sensitive mutants of Escherichia coli with altered ribosomal proteins.

Authors:  K Isono; J Krauss; Y Hirota
Journal:  Mol Gen Genet       Date:  1976-12-22

8.  Effect of the RelA gene on the synthesis of individual proteins in vivo.

Authors:  A V Furano; F P Wittel
Journal:  Cell       Date:  1976-05       Impact factor: 41.582

9.  Selective separation procedure for determination of ribosomal proteins L7 and L12.

Authors:  K Li; A R Subramanian
Journal:  Anal Biochem       Date:  1975-03       Impact factor: 3.365

10.  Nucleotide sequences from tryptophan messenger RNA of Escherichia coli: the sequence corresponding to the amino-terminal region of the first polypeptide specified by the operon.

Authors:  M J Bronson; C Squires; C Yanofsky
Journal:  Proc Natl Acad Sci U S A       Date:  1973-08       Impact factor: 11.205
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  38 in total

1.  Isolation and mapping of Escherichia coli mutations conferring resistance to division inhibition protein DicB.

Authors:  C Labie; F Bouché; J P Bouché
Journal:  J Bacteriol       Date:  1989-08       Impact factor: 3.490

2.  Autogenous regulation of the RNA polymerase beta subunit of Escherichia coli occurs at the translational level in vivo.

Authors:  L Passador; T Linn
Journal:  J Bacteriol       Date:  1989-11       Impact factor: 3.490

3.  A missense mutation in the rpoC gene affects chromosomal replication control in Escherichia coli.

Authors:  S K Petersen; F G Hansen
Journal:  J Bacteriol       Date:  1991-08       Impact factor: 3.490

4.  Evidence that Rho and NusA are involved in termination in the rplL-rpoB intercistronic region.

Authors:  G Ralling; T Linn
Journal:  J Bacteriol       Date:  1987-05       Impact factor: 3.490

5.  Insertional mutagenesis of a plasmid-borne Escherichia coli rpoB gene reveals alterations that inhibit beta-subunit assembly into RNA polymerase.

Authors:  R Landick; A Colwell; J Stewart
Journal:  J Bacteriol       Date:  1990-06       Impact factor: 3.490

6.  In vivo cloning of a carboxy-terminal rpoB allele which confers altered transcriptional properties.

Authors:  G C Rowland; P P Lim; R E Glass
Journal:  Folia Microbiol (Praha)       Date:  1995       Impact factor: 2.099

7.  Heat stress in the presence of low RNA polymerase activity increases chromosome copy number of Escherichia coli.

Authors:  E C Guzman; A Jimenez-Sanchez; E Orr; R H Pritchard
Journal:  Mol Gen Genet       Date:  1988-05

8.  Genetic studies on the beta subunit of Escherichia coli RNA polymerase. IV. Structure-function correlates.

Authors:  V Nene; R E Glass
Journal:  Mol Gen Genet       Date:  1984

9.  Thermoinducible filamentation in Escherichia coli due to an altered RNA polymerase beta subunit is suppressed by high levels of ppGpp.

Authors:  D Vinella; R D'Ari
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490

10.  Isolation of a recombinant lambda phage carrying nusA and surrounding region of the Escherichia coli K-12 chromosome.

Authors:  E W Holowachuk; J D Friesen
Journal:  Mol Gen Genet       Date:  1982
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