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Literature DB >> 799248

Isolation and characterization of temperature-sensitive mutants of Escherichia coli with altered ribosomal proteins.

Abstract

The ribosomal proteins of temperature-sensitive mutants of Escherichia coli isolated independently after mutagenesis with nitrosoguanidine were analyzed by two-dimensional gel electrophoresis. Out of 400 mutants analyzed, 60 mutants (15%) showed alterations in a total of 22 different ribosomal proteins. The proteins altered in these mutants are S2, S4, S6, S7, S8, S10, S15, S16, S18, L1, L3, L6, L10, L11, L14, L15, L17, L18, L19, L22, L23 and L24. A large number of them (25 mutants) have mutations in protein S4 of the small subunit, while four mutants showed alterations in protein L6 of the large subunit. The importance of these mutants for structural and functional analyses of ribosomes is discussed.

Entities: Chemical Gene Species

Mesh：

Substances：
Ribosomal Proteins

Year: 1976 PMID： 799248 DOI： 10.1007/bf00268531

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

26 in total

1. A Thermosensitive mutant defective in ribosomal 30 S subunit assembly.

Authors: R Rosset; C Vola; J Feunteun; R Monier
Journal: FEBS Lett Date: 1971-10-15 Impact factor: 4.124

2. Late steps in the assembly of 30 S ribosomal proteins in vivo in a spectinomycin-resistant mutant of Escherichia coli.

Authors: H Nashimoto; H Uchida
Journal: J Mol Biol Date: 1975-08-15 Impact factor: 5.469

3. Isolation of transducing phage carrying rps T, the structural gene for ribosomal protein S20.

Authors: J D Friesen; J Parker; R J Watson; N P Fiil; S Pedersen
Journal: Mol Gen Genet Date: 1976-02-27

4. Pleiotropic effects of ribosomal protein s4 studied in Escherichia coli mutants.

Authors: M Olsson; L Isaksson; C G Kurland
Journal: Mol Gen Genet Date: 1974

5. Letter: Binding of 50 S ribosomal subunit proteins to 23 S RNA of Escherichia coli.

Authors: R A Garret; S Müller; P Spierer; R A Zimmermann
Journal: J Mol Biol Date: 1974-09-15 Impact factor: 5.469

6. Genetic analysis of an alteration of ribosomal protein S20 in revertants of an alanyl-tRNA-synthetase mutant of Escherichia coli.

Authors: A Böck; D Ruffler; W Piepersberg; H G Wittmann
Journal: Mol Gen Genet Date: 1974

7. The structural gene for ribosomal protein S18 in Escherichia coli. III. Mapping outside the ribosomal protein gene cluster at minute 84 on the genome.

Authors: M DeWilde; F Michel; K Broman
Journal: Mol Gen Genet Date: 1974

8. Chromosome replication and the division cycle of Escherichia coli B/r.

Authors: S Cooper; C E Helmstetter
Journal: J Mol Biol Date: 1968-02-14 Impact factor: 5.469

9. Correlation of 30S ribosomal proteins of Escherichia coli isolated in different laboratories.

Authors: H G Wittmann; G Stöfflet; I Hindennach; C G Kurland; E A Birge; L Randall-Hazelbauer; M Nomura; E Kaltschmidt; S Mizushima; R R Traut; T A Bickle
Journal: Mol Gen Genet Date: 1971

10. Ribosomal proteins. XXVI. The number of specific protein binding sites on 16 s and 23 s RNA of Escherichia coli.

Authors: G Stöffler; L Daya; K H Rak; R A Garrett
Journal: J Mol Biol Date: 1971-12-14 Impact factor: 5.469

39 in total

1. Temperature-sensitive sec mutants of Escherichia coli: inhibition of protein export at the permissive temperature.

Authors: K Ito; Y Hirota; Y Akiyama
Journal: J Bacteriol Date: 1989-03 Impact factor: 3.490

2. Mutagenesis of ribosomal protein S8 from Escherichia coli: defects in regulation of the spc operon.

Authors: I Wower; M P Kowaleski; L E Sears; R A Zimmermann
Journal: J Bacteriol Date: 1992-02 Impact factor: 3.490

3. The rice nuclear gene WLP1 encoding a chloroplast ribosome L13 protein is needed for chloroplast development in rice grown under low temperature conditions.

Authors: Jian Song; Xiangjin Wei; Gaoneng Shao; Zhonghua Sheng; Daibo Chen; Congli Liu; Guiai Jiao; Lihong Xie; Shaoqing Tang; Peisong Hu
Journal: Plant Mol Biol Date: 2013-10-15 Impact factor: 4.076

4. Expression of Escherichia coli ribosomal protein and RNA polymerase genes cloned on plasmids.

Authors: N P Fiil; D Bendiak; J Collins; J D Friesen
Journal: Mol Gen Genet Date: 1979-05-23

5. Genes encoding ribosomal proteins S16 and L19 form a gene cluster at 56.4 min in Escherichia coli.

Authors: K Isono
Journal: Mol Gen Genet Date: 1978-10-24

6. Mutations affecting the structural genes and the genes coding for modifying enzymes for ribosomal proteins in Escherichia coli.

Authors: S Isono; K Isono
Journal: Mol Gen Genet Date: 1978-09-20