Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Isolation of a recombinant lambda phage carrying nusA and surrounding region of the Escherichia coli K-12 chromosome.

Literature DB >> 6294462

Isolation of a recombinant lambda phage carrying nusA and surrounding region of the Escherichia coli K-12 chromosome.

Abstract

A recombinant bacteriophage lambda, lambda argG-6, has been isolated which carries the argG gene and neighbouring loci on an EcoRI-generated 15.5 Kb DNA fragment from the Escherichia coli chromosome. The locations of the argG, nusA and pnp genes on the 15.5 Kb DNA fragment have been determined. In the case of nusA, a Tn5 insertion and sub-cloning of restriction fragments were used to locate the gene. The gene products of nusA and pnp have been identified on one- and two-dimensional polyacrylamide gels. The clockwise gene order was found to be argG-nusA-pnp.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1982 PMID： 6294462 DOI： 10.1007/bf00331126

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

38 in total

1. Organization of genes for transcription and translation in the rif region of the Escherichia coli chromosome.

Authors: M Yamamoto; M Nomura
Journal: J Bacteriol Date: 1979-01 Impact factor: 3.490

2. EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system.

Authors: P Leder; D Tiemeier; L Enquist
Journal: Science Date: 1977-04-08 Impact factor: 47.728

3. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors: P A Sharp; B Sugden; J Sambrook
Journal: Biochemistry Date: 1973-07-31 Impact factor: 3.162

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

5. Cloning of E. coli pnp gene from an episome.

Authors: C Portier; C Migot; M Grumberg-Manago
Journal: Mol Gen Genet Date: 1981

6. The nus mutations affect transcription termination in Escherichia coli.

Authors: D F Ward; M E Gottesman
Journal: Nature Date: 1981-07-16 Impact factor: 49.962

7. The isolation of structural genes from libraries of eucaryotic DNA.

Authors: T Maniatis; R C Hardison; E Lacy; J Lauer; C O'Connell; D Quon; G K Sim; A Efstratiadis
Journal: Cell Date: 1978-10 Impact factor: 41.582

Review 8. Linkage map of Escherichia coli K-12, edition 6.

Authors: B J Bachmann; K B Low
Journal: Microbiol Rev Date: 1980-03

Review 9. Autogenous and post-transcriptional regulation of RNA polymerase synthesis.

Authors: A Ishihama; R Fukuda
Journal: Mol Cell Biochem Date: 1980-08-16 Impact factor: 3.396

10. L factor that is required for beta-galactosidase synthesis is the nusA gene product involved in transcription termination.

Authors: J Greenblatt; J Li; S Adhya; D I Friedman; L S Baron; B Redfield; H F Kung; H Weissbach
Journal: Proc Natl Acad Sci U S A Date: 1980-04 Impact factor: 11.205

8 in total

7. Application of phage lambda technology to Salmonella typhimurium. Construction of a lambda-sensitive Salmonella strain.

Authors: A Harkki; E T Palva
Journal: Mol Gen Genet Date: 1984

8. The complete sequence of the MHC class II chain RT.1D alpha u of the diabetic BB rat: mRNA levels of RT1.D alpha in lymphocytes.

Authors: E W Holowachuk; M K Greer; D R Martin
Journal: Nucleic Acids Res Date: 1987-12-23 Impact factor: 16.971