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Literature DB >> 378979

Isolation and characterization of replication-deficient mutants of ColE1 plasmids.

Abstract

Replication-defective mutants of plasmid ColE1 were isolated from a chimeric plasmid formed by ligating a temperature-sensitive replication derivative of pSC101, pHSG1, with a ColE1-Tn3-containing plasmid. The replication-defective ColE1 mutants isolated were all spontaneous deletion mutants that had lost the ColE1 replication origin and regions adjacent to it. The extent of a deletion was determined by analyzing restriction endonuclease-generated deoxyribonucleic acid fragments of the ColE1 plasmid component of the chimeras by both agarose and polyacrylamide gel electrophoresis. None of the chimeras containing the replication-defective ColE1 mutants was able to replicate in the presence of chloramphenicol. The expression of ColE1 incompatibility was either markedly reduced or not detectable in the replication mutants isolated.

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Year: 1979 PMID： 378979 PMCID： PMC216909 DOI： 10.1128/jb.139.2.597-607.1979

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

19 in total

1. Origin of replication of pBR345 plasmid DNA.

Authors: F Bolivar; M C Betlach; H L Heyneker; J Shine; R L Rodriguez; H W Boyer
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

2. Isolation, mapping, and examination of effects of TnA insertions in ColE1 plasmids.

Authors: J Inselburg
Journal: J Bacteriol Date: 1977-01 Impact factor: 3.490

Review 3. On plasmid incompatibility.

Authors: R P Novick; F C Hoppensteadt
Journal: Plasmid Date: 1978-09 Impact factor: 3.466

4. Isolation and genetic analysis of deletion mutants of colicin E1 plasmids carrying a TnA insertion.

Authors: J Inselburg; P Ware
Journal: J Bacteriol Date: 1977-10 Impact factor: 3.490

5. Mutations of temperature sensitivity in R plasmid pSC101.

Authors: T Hashimoto-Gotoh; M Sekiguchi
Journal: J Bacteriol Date: 1977-08 Impact factor: 3.490

6. Origin of replication of colicin E1 plasmid DNA.

Authors: J I Tomizawa; H Ohmori; R E Bird
Journal: Proc Natl Acad Sci U S A Date: 1977-05 Impact factor: 11.205

7. Random replication and random assortment model for plasmid incompatibility in bacteria.

Authors: K Ishii; T Hashimoto-Gotoh; K Matsubara
Journal: Plasmid Date: 1978-09 Impact factor: 3.466

8. Site-specific DNA deletions occurring adjacent to the termini of a transposable ampicillin resistance element (Tn3).

Authors: P D Nisen; D J Kopecko; J Chou; S N Cohen
Journal: J Mol Biol Date: 1977-12-25 Impact factor: 5.469

9. ColE1 plasmid incompatibility: localization and analysis of mutations affecting incompatibility.

Authors: T Hashimoto-Gotoh; J Inselburg
Journal: J Bacteriol Date: 1979-08 Impact factor: 3.490

10. ColE1 plasmid mutants affecting growth of an Escherichia coli recB recC sbcB mutant.

Authors: J Inselburg
Journal: J Bacteriol Date: 1978-01 Impact factor: 3.490

7 in total

1. Maintenance and genetic stability of vector plasmids pBR322 and pBR325 in Escherichia coli K12 strains grown in a chemostat.

Authors: D Noack; M Roth; R Geuther; G Müller; K Undisz; C Hoffmeier; S Gáspár
Journal: Mol Gen Genet Date: 1981

Isolation and characterization of replication-deficient mutants of ColE1 plasmids.

1. Origin of replication of pBR345 plasmid DNA.

2. Isolation, mapping, and examination of effects of TnA insertions in ColE1 plasmids.

Review 3. On plasmid incompatibility.

4. Isolation and genetic analysis of deletion mutants of colicin E1 plasmids carrying a TnA insertion.

5. Mutations of temperature sensitivity in R plasmid pSC101.

6. Origin of replication of colicin E1 plasmid DNA.

7. Random replication and random assortment model for plasmid incompatibility in bacteria.

8. Site-specific DNA deletions occurring adjacent to the termini of a transposable ampicillin resistance element (Tn3).

9. ColE1 plasmid incompatibility: localization and analysis of mutations affecting incompatibility.

10. ColE1 plasmid mutants affecting growth of an Escherichia coli recB recC sbcB mutant.

1. Maintenance and genetic stability of vector plasmids pBR322 and pBR325 in Escherichia coli K12 strains grown in a chemostat.

2. cea-kil operon of the ColE1 plasmid.

3. ColE1 copy number mutants.

4. ColE1 plasmid incompatibility: localization and analysis of mutations affecting incompatibility.

5. Functional relationship between parts of the replication region of plasmid ColE1.

6. Alternative forms of lethality in mitomycin C-induced bacteria carrying ColE1 plasmids.

7. Selection and characterization of ColE1 plasmid mutants that exhibit altered stability and replication.