Literature DB >> 6278246

Maintenance and genetic stability of vector plasmids pBR322 and pBR325 in Escherichia coli K12 strains grown in a chemostat.

D Noack, M Roth, R Geuther, G Müller, K Undisz, C Hoffmeier, S Gáspár.   

Abstract

The maintenance and genetic stability of the vector plasmids pBR322 and pBR325 in two genetically different Escherichia coli hosts were studied during chemostat cultivation with glucose and ammonium chloride limitation and at two different dilution rates. The plasmid pBR322 was stably maintained under all growth conditions tested. However pBR325 segregated from both hosts preferentially during glucose limitation and at low dilution rate. In addition to this general segregation process a separate loss of tetracycline resistance was observed. The remaining plasmid conferred resistance to ampicillin and chloramphenicol only, without any remarkable alteration of its molecular weight. Cultivation conditions in the chemostat were found that allowed the stable genetic inheritance of both plasmids in the hosts studied.

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Year:  1981        PMID： 6278246     DOI： 10.1007/bf00271207

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  18 in total

1.  Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance.

Authors:  P A Meacock; S N Cohen
Journal:  Cell       Date:  1980-06       Impact factor: 41.582

2.  R-plasmid persistence in Escherichia coli grown in chemostat cultures [proceedings].

Authors:  J T Wouters; J G van Andel
Journal:  Antonie Van Leeuwenhoek       Date:  1979       Impact factor: 2.271

3.  Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

Authors:  M Dagert; S D Ehrlich
Journal:  Gene       Date:  1979-05       Impact factor: 3.688

4.  The influence of the growth environment on the stability of a drug resistance plasmid in Escherichia coli K12.

Authors:  D Godwin; J H Slater
Journal:  J Gen Microbiol       Date:  1979-03

5.  Frequency-Dependent Selection for Plasmid-Containing Cells of ESCHERICHIA COLI.

Authors:  J Adams; T Kinney; S Thompson; L Rubin; R B Helling
Journal:  Genetics       Date:  1979-04       Impact factor: 4.562

6.  Persistence of the pBR 322 plasmid in Escherichia coli K 12 grown in chemostat cultures.

Authors:  J T Wouters; F L Driehuis; P J Polaczek; M L van Oppenraay; J G van Andel
Journal:  Antonie Van Leeuwenhoek       Date:  1980       Impact factor: 2.271

7.  Generation time-prolonging R plasmids: correlation between increases in the generation time of Escherichia coli caused by R plasmids and their molecular size.

Authors:  P Zünd; G Lebek
Journal:  Plasmid       Date:  1980-01       Impact factor: 3.466

8.  Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325.

Authors:  X Soberon; L Covarrubias; F Bolivar
Journal:  Gene       Date:  1980-05       Impact factor: 3.688

9.  Maintenance of some ColE1-type plasmids in chemostat culture.

Authors:  I M Jones; S B Primrose; A Robinson; D C Ellwood
Journal:  Mol Gen Genet       Date:  1980

10.  Phenotypic stability of trp operon recombinant plasmids in Escherichia coli.

Authors:  T Imanaka; H Tsunekawa; S Aiba
Journal:  J Gen Microbiol       Date:  1980-05
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  24 in total

1.  Plasmid macro-evolution: selection of deletions during adaptation in a nutrient-limited environment.

Authors:  R I Modi; C M Wilke; R F Rosenzweig; J Adams
Journal:  Genetica       Date:  1991       Impact factor: 1.082

2.  Plasmid pIMI38--the pBR322 derivative with increased stability in E. coli cells.

Authors:  O Navrátil; K Cejka; M Strnadová; C Hoffemeier; H Braná; J Hubácek
Journal:  Folia Microbiol (Praha)       Date:  1988       Impact factor: 2.099

Review 3.  The future of metabolic engineering and synthetic biology: towards a systematic practice.

Authors:  Vikramaditya G Yadav; Marjan De Mey; Chin Giaw Lim; Parayil Kumaran Ajikumar; Gregory Stephanopoulos
Journal:  Metab Eng       Date:  2012-05       Impact factor: 9.783

4.  Selection and fitness in bacteriocin-producing bacteria.

Authors:  G A Dykes; J W Hastings
Journal:  Proc Biol Sci       Date:  1997-05-22       Impact factor: 5.349

5.  Plasmid inheritability and biomass production: comparison between free and immobilized cell cultures of Escherichia coli BZ18(pTG201) without selection pressure.

Authors:  P de Taxis du Poët; P Dhulster; J N Barbotin; D Thomas
Journal:  J Bacteriol       Date:  1986-03       Impact factor: 3.490

6.  Influence of immobilization on the stability of pTG201 recombinant plasmid in some strains of Escherichia coli.

Authors:  M Nasri; S Sayadi; J N Barbotin; P Dhulster; D Thomas
Journal:  Appl Environ Microbiol       Date:  1987-04       Impact factor: 4.792

7.  Genetic analysis of a plasmid-encoded, host genotype-specific enhancement of bacterial fitness.

Authors:  R E Lenski; S C Simpson; T T Nguyen
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490

8.  Large-scale preparation of ribulosebisphosphate carboxylase from a recombinant system in Escherichia coli characterized by extreme plasmid instability.

Authors:  J Pierce; S Gutteridge
Journal:  Appl Environ Microbiol       Date:  1985-05       Impact factor: 4.792

9.  Stability of a plasmid F Trim in populations of a recombination-deficient strain of Escherichia coli in continuous culture.

Authors:  R E Ashby; K A Stacey
Journal:  Antonie Van Leeuwenhoek       Date:  1984       Impact factor: 2.271

10.  Stability of Escherichia coli strains harboring recombinant plasmids for L-threonine production.

Authors:  B C Nudel; M G Pueyo; N D Judewicz; A M Guilietti
Journal:  Antonie Van Leeuwenhoek       Date:  1989-10       Impact factor: 2.271
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