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Literature DB >> 271952

Origin of replication of pBR345 plasmid DNA.

F Bolivar, M C Betlach, H L Heyneker, J Shine, R L Rodriguez, H W Boyer.

Abstract

A small (approximately 1100 base pairs) ColE1-type plasmid, pBR345, was constructed from plasmid pMB1 by a series of in vitro recombinant manipulations. Approximately 9% of the supercoiled pBR345 DNA obtained from cultures amplified with chloramphenicol appears to be replicative intermediates with replicating "eye" structures of uniform size. Results obtained from electron microscopy and biochemical analyses have enabled us to localize the origin of replication at the same position as that reported for ColE1. A sequence of 420 nucleotides surrounding this origin has been determined. A comparison between this sequence and the one determined for the origin of replication of ColE1 is presented.

Entities: Chemical

Mesh：

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Year: 1977 PMID： 271952 PMCID： PMC431677 DOI： 10.1073/pnas.74.12.5265

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

20 in total

1. THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. IV. AN EXONUCLEASE ACTIVITY PRESENT IN PURIFIED PREPARATIONS OF DEOXYRIBONUCLEIC ACID POLYMERASE.

Authors: I R LEHMAN; C C RICHARDSON
Journal: J Biol Chem Date: 1964-01 Impact factor: 5.157

5. Structural and functional analysis of cloned DNA segments containing the replication and incompatibility regions of a miniplasmid derived from a copy number mutant of NR1.

Authors: D P Taylor; S N Cohen
Journal: J Bacteriol Date: 1979-01 Impact factor: 3.490

Origin of replication of pBR345 plasmid DNA.

1. THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. IV. AN EXONUCLEASE ACTIVITY PRESENT IN PURIFIED PREPARATIONS OF DEOXYRIBONUCLEIC ACID POLYMERASE.

2. Synthetic lac operator DNA is functional in vivo.

3. Studies on bacteriophage fd DNA. IV. The sequence of messenger RNA for the major coat protein gene.

4. A new method for sequencing DNA.

5. Isolation, mapping, and examination of effects of TnA insertions in ColE1 plasmids.

6. Origin of replication of colicin E1 plasmid DNA.

7. Replication of colicin E1 plasmid DNA in cell extracts. Origin and direction of replication.

8. Cleavage map of colicin E1 plasmid.

9. On the mechanism of genetic recombination: electron microscopic observation of recombination intermediates.

10. Synthesis and characterization of new psoralen derivatives with superior photoreactivity with DNA and RNA.

1. Rifampin-resistant replication of pBR322 derivatives in Escherichia coli cells induced for the SOS response.

Review 2. Replication and control of circular bacterial plasmids.

Review 3. DNA-protein interactions during replication of genetic elements of bacteria.

4. In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites.

5. Structural and functional analysis of cloned DNA segments containing the replication and incompatibility regions of a miniplasmid derived from a copy number mutant of NR1.

6. The nucleotide sequence of a DNA fragment from the replication origin of the antibiotic resistance factor R1drd19.

7. Interaction of the tight-binding I12-X86 lac repressor with non operator DNA: salt dependence of complex formation.

8. Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome.

9. Isolation and characterization of replication-deficient mutants of ColE1 plasmids.

10. Initiation of DNA replication in a ColE1-type plasmid: isolation of mutations in the ori region.